FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109355749
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTGATACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT17184321.5714144118751363No Hit
ATAGTGATACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2371180.21683176437299148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATAC2488300.084.334194
TAGTGAT2533250.082.578312
TGATACT2571300.081.586075
AGTGATA2567950.081.38513
ATAGTGA2642750.079.241111
GATACTG2391450.077.6918956
TACTGGC2397600.074.655378
ATACTGG2420450.074.3819667
ACTGGCG2911850.061.728549
GATACTT558800.044.84566
GCGATAA2081550.043.9515936-37
CGATAAC2081250.043.50572638-39
ATAACCG2083600.043.3558840-41
AGCGATA2109800.042.9466436-37
TAACCGG2124700.042.8323440-41
CGGAGTA2117850.042.22624244-45
TAAGACG2173850.041.9715264-65
ATCTCGA2198950.041.4770718-19
AGTCGCC2187550.041.26827228-29
GATAACC2221150.041.21019438-39
Produced by FastQC (version 0.11.5)