FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110865345
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5183750.46757171954861104No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1392730.1256235661378224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG709200.075.0890359
CCCCCTA1106700.065.5175
AATCCCC1212400.060.4333762
GAATCCC1224150.059.8702621
CCCCTAG1002400.058.342026
CCCTAGG963200.055.7754637
ATCCCCC1314000.055.477823
CCTAGGC1103900.048.7228058
GCGATAA687500.040.4953836-37
TCCCCCT1842200.039.8542984
CGATAAC688500.038.95276638-39
ATAACCG696350.038.57834640-41
TAACCGG732800.038.0853640-41
AGCGATA707050.037.93473436-37
CGGAGTA740850.037.02160644-45
TAAGACG774650.035.87565664-65
TAGGCGT762400.035.18321210-11
CCCCTAT409150.034.9996266
AGTCGCC792350.034.97414428-29
GAGTCGC782250.034.1415328-29
Produced by FastQC (version 0.11.5)