FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110865345
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5107300.4606759668677349No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1399770.12625857070124122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG792850.066.92029
CCCCCTA1179750.061.091615
GAATCCC1233150.059.235231
AATCCCC1275300.057.226042
CCCCTAG1092550.052.869666
ATCCCCC1379850.052.4942363
CCCTAGG1040600.051.3099337
CCTAGGC1168200.045.819818
GCGATAA780000.039.32184236-37
TCCCCCT1878750.038.842224
TAACCGG827750.037.07451640-41
CCCCTAT406950.036.0045136
CGGAGTA833800.035.8616444-45
AGTCGCC865300.034.76806628-29
CGATAAC776600.034.4449838-39
TAAGACG886550.034.39410464-65
ATAACCG789400.033.91125540-41
ATCTCGA914700.033.62934518-19
AGCGATA801000.033.44600736-37
AGGCGTC929750.033.13091710-11
Produced by FastQC (version 0.11.5)