FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108867239
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTTAATCGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9475900.8704087737542421No Hit
CAGCTTAATCGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2920340.2682478243064472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG1490550.088.589895
TAATCGG1190800.086.772016
AATCGGG1183800.081.175527
AGCTTAA1650400.080.199232
GCTTAAT1651700.080.061673
CTTAATC1662550.079.5877464
TAATCGT368700.078.190696
ATCGGGC1262850.076.1205448
CAGCTTA1755500.075.533451
TCGGGCG1328900.072.415999
AATCGTG424550.068.0472647
ATCGTGA484600.059.7228788
TCGTGAA497450.058.247159
ATCGGTA146500.049.8910528
AATCGGT158500.046.623197
GCGATAA1154650.042.22944636-37
CGATAAC1155200.041.55160538-39
ATAACCG1163200.041.27009640-41
AGCGATA1173850.040.86303336-37
TAACCGG1229550.039.72289340-41
Produced by FastQC (version 0.11.5)