FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110783571
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3158310.2850883006831401No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1475410.1331794946382438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG544350.058.5394029
CCCCCTA959400.053.6337245
GAATCCC1028300.050.575451
AATCCCC1055250.049.5804752
CCCCTAG830600.044.6741376
ATCCCCC1174650.044.1278153
CCCTAGG823500.039.2601627
GCGATAA515250.037.28233736-37
CCCCTAT431850.034.398266
TAACCGG561350.034.33611740-41
CGGAGTA582400.032.70635644-45
CCTAGGC1005900.032.122868
TAAGACG600750.031.9936964-65
CGATAAC518000.031.30360638-39
ATAACCG521200.031.0986240-41
AGTCGCC616650.031.04666528-29
TCCCCCT1701900.030.8198664
AGCGATA537250.030.18167936-37
TTCGCGT266850.028.93929718-19
AGGCGTC670800.028.80610510-11
Produced by FastQC (version 0.11.5)