FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114619543
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1938650.16913782320699008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG342500.058.752239
CCCCCTA598050.044.987085
CCCCTAG591200.037.394756
AATCCCC744800.036.5979232
GCGATAA298000.035.18301836-37
GAATCCC772450.035.123421
CGATAAC299650.033.896138-39
ATAACCG304850.033.27070640-41
ATCCCCC818300.033.1863333
TAACCGG316350.033.02961740-41
CCCTAGG621400.032.8259477
AGCGATA324550.031.37558736-37
TAAGACG356900.029.45165464-65
CGGAGTA357850.029.046144-45
CGACCAA377200.026.95857270-71
AGTCGCC400250.026.12190428-29
GAGTCGC392250.025.86144328-29
AACCGGA396100.025.61207242-43
TAGGCGT398200.025.52957210-11
AGACGAC419900.025.15711866-67
Produced by FastQC (version 0.11.5)