FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114619543
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1856780.16199506222075935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG360350.054.3866739
CCCCCTA623600.042.027585
AATCCCC758050.035.201042
GCGATAA317150.034.99398436-37
CCCCTAG615350.034.7812846
GAATCCC762800.034.7298931
TAACCGG350550.032.0266740-41
ATCCCCC832350.031.830593
CCCTAGG641650.031.0027357
CGATAAC320050.030.8567438-39
ATAACCG329800.030.2472840-41
TAAGACG376300.029.85757664-65
CGGAGTA383850.028.93831844-45
AGCGATA344500.028.72127536-37
AGTCGCC428350.026.14100328-29
AGACGAC450550.024.8999666-67
CGACCAA401500.024.84006770-71
CCTAGGC818400.024.5320958
TAGGCGT409250.024.32131610-11
GAGTCGC415200.024.05754328-29
Produced by FastQC (version 0.11.5)