FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149424413
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3469190.23217022776592738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG73300.029.69738835
CCGACCG79050.027.66815634
CGTGCCA312000.024.6238771
GTGCTAG425400.024.0878811
TATGCCG98050.022.37700549
TAGTTAC528250.019.6768155
GTGCCAG4331550.018.7069781
GTTACCT6405850.018.313647
AGTTACC6087300.018.161226
TACCTTG6522250.018.1312089
CGGTTAC253300.018.1276823
TGCCAGT4819950.017.538652
ACCTTGT6811050.017.3876310
CCAGTTA5277800.017.3066524
GATCGGA167000.016.9672931
CAGTTAC5953550.016.8076525
CCTTGTT7115650.016.69761511
TTACCTT7139350.016.6761118
GCCAGTT5415900.016.4291023
CTAGTTA645000.016.0403544
Produced by FastQC (version 0.11.5)