FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149424413
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTGGGTTCGGTCCTCAGCTCTGGAAAAAAAAA2574000.17226100797866276No Hit
GGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTGGGTTCGGTCCTCAGCTCTGGAAAAAAA1724770.11542759080472347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTTA2125400.034.361791
GATTTAG2503850.026.6781752
ATTTAGC4425200.023.439621
CGCCGTA90500.023.09220746
TTAGCTC3819850.022.4654753
TAGCTCA4038350.021.3810424
TAGAGCG2875700.021.11209515
TCGGTCC3976700.021.0722350
GGGATTT1228500.021.0238041
GTTCGGT2941800.020.9780648
CGGTCCT4638450.020.86315351
CGCTTGC2632750.020.8410220
ATCGGTC1090350.020.83302749
TTCGGTC2953500.020.78559549
GCGTCGT104600.020.70904511
GGGTTCG3214950.020.64572746
GGTTCGG3257900.020.59494447
GCGCTTG2619700.020.58780919
AGAGCGC3043750.020.16166516
GTAGAGC3020500.020.08384514
Produced by FastQC (version 0.11.5)