FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153701659
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1869650.12164149770172616No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1554510.10113814061044064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT112550.022.73731811
CGCCGTA112850.021.49475746
GCCGTAT118900.020.72028547
CGTATCA135000.018.14673649
AGCGTCG149700.017.32528110
CGTGTAG158650.016.00057815
GTTCGGT247150.014.547255548
TCGGTCC361400.014.29240650
TCGTGTA183900.014.12211314
GTCGCCG183350.014.09519744
TGGTCGC186500.013.44936742
CCGTATC190900.013.37518348
GTCGTGT194850.013.20458713
CGGTCCT465100.013.16002351
TAGAGCG205150.012.99588715
GGGTTCG312450.012.98690346
GATCGGA205350.012.7296311
GGTCGCC205150.012.56361643
TCTCGGT206450.012.51771236
ATCGGTC143050.011.91552249
Produced by FastQC (version 0.11.5)