FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164874776
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97800.017.49569349
GTTACCT1527900.015.6698617
AGTTACC1443600.015.42356
TACCTTG1623950.014.7945989
TTACCTT1860800.013.0926148
CAGTTAC1599450.012.6323445
ACCTTGT1933900.012.5733710
CCAGTTA1474950.012.4191494
CCTTGTT2224350.011.128736511
GATCGGA196950.010.8969821
GCCAGTT1789950.010.0384493
CGGTTAC114100.09.8594323
TCGTATG193000.09.77727746
GTGCCAG1725400.09.6043831
CTTGTTT2678700.09.5652812
AGACCGA187550.09.56511131
AAGAGCG241500.09.1138977
GAGCGGT251250.08.8292129
AATGCCG250350.08.82010624
TGCCAGT1994950.08.6299982
Produced by FastQC (version 0.11.5)