FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164874776
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2606040.15806177653275477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT619450.019.05785650
GGGTTCG724750.018.61729848
TCGGTCC906000.018.55926752
CGGTCCT1084850.018.188953
TAGAGCG453950.017.73631317
GGTTCGG789250.017.2576149
GGATTTA826700.016.647421
CGCCGTA102200.016.41062746
GCGTCGT106250.016.10063711
ATCGGTC323900.016.09717651
GATTTAG811350.015.9893142
TTCGGTC745150.015.77237651
GCAAGCG862600.015.25675332
CGCTTGC502350.015.1065622
CGCAAGG1028150.015.10619137
AAGCGCA1061400.014.84763434
GATCGGT372500.014.76689750
GCCGTAT113650.014.69658447
GCGCTTG494700.014.22450521
AGCGCAA1146350.013.80454735
Produced by FastQC (version 0.11.5)