FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169870649
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3156760.18583316297331626No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1872410.11022563409409238No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1867110.10991363198947925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT137100.022.2145611
CGCCGTA137350.021.73021346
GCCGTAT148350.020.25850147
CGTATCA153250.019.72258649
GTTCGGT591850.018.24160850
TCGGTCC851700.017.79325352
AGCGTCG177450.017.61027510
CGGTCCT1013750.017.33125753
GGGTTCG701400.017.04495248
GGTTCGG714450.016.86890849
TAGAGCG405050.016.64130617
CCGTATC185550.016.58678448
CGTGTAG189050.016.14675115
ATCGGTC288500.015.60819251
TTCGGTC686950.015.58133151
GTCGCCG217500.014.34008644
GCAAGCG823300.014.24310632
GGATTTA746450.014.2392851
GATTTAG713000.014.162682
CGCAAGG963550.013.82513937
Produced by FastQC (version 0.11.5)