FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158882142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2402540.15121523223170039No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2354900.1482167832304275No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1999010.12581716074799645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCC646450.018.61019750
GTTCGGT426500.018.33422348
CGGTCCT790250.017.95062851
TTCGGTC473050.016.4072249
TAGAGCG312550.016.13114715
GGGTTCG554050.015.9634246
ATCGGTC278700.015.63936149
GGTTCGG576800.015.49569947
GCGTCGT98400.015.17727711
GCAAGCG619750.015.03899130
CGCTTGC357300.014.50875520
GATCGGT314200.014.35459848
CGCAAGG726050.013.61916335
AAGCGCA760100.013.452929532
GGATCGG449550.013.41837947
GGGATCG461500.013.24259846
GCCGTAT111000.013.15085647
AGCGCAA793150.012.8221333
TTAGCTC629400.012.7764943
GGTCCTC1194700.012.41096952
Produced by FastQC (version 0.11.5)