FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166274171
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG120800.012.50740546
TATGCCG130900.011.75384249
GATCGGA181250.010.1514541
AGACCGC157250.09.80690531
GACCGCA181950.08.5704132
ATCGGAA243650.07.8858642
CGTATGC209950.07.820799447
GAGCGGT246800.07.6607229
AAGAGCG255100.07.65302947
ACCGCAA208600.07.64108333
CTCGTAT206150.07.446418345
CGCAACT216450.07.252280735
CGGTTCA263450.07.17768212
CCGCAAC226900.06.994401534
TCGACAG286800.06.953142638
GTACGAT191750.06.892101322
TTTCGAC292650.06.884701736
TACGGGC309950.06.845646439
ACCTACG192100.06.823733368
ATACGGG329450.06.81748138
Produced by FastQC (version 0.11.5)