FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170379530
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3963150.23260716824374383No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1773430.1040870343990267No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1728470.10144821974799438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT641700.016.87619450
TCGGTCC951750.016.16903952
GGGTTCG761350.015.69281448
CGGTCCT1150600.015.44927853
GGTTCGG782350.015.27168449
CGCCGTA104300.015.25273546
TAGAGCG489300.014.97124617
TTCGGTC737250.014.61462951
ATCGGTC332750.014.48932151
GCGTCGT116800.014.20347711
GATTTAG772800.014.1863712
GGATTTA820650.014.0139981
GATCGGT381100.013.07620850
CGCAAGG1052150.012.99240937
GCAAGCG914400.012.96361232
CGCTTGC535050.012.94571522
GCCGTAT127250.012.82787647
AAGCGCA1105750.012.55127834
GCGCTTG536150.012.03767321
AGCGCAA1153800.011.999535
Produced by FastQC (version 0.11.5)