FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005730111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005730111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166005385
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCT2084500.018.240677
TATGCCG112250.018.0041649
AGTTACC1995850.017.763486
TACCTTG2227150.017.1680649
CCAGTTA1854200.015.7474194
CAGTTAC2108800.015.253965
ACCTTGT2586650.014.92839610
TTACCTT2680800.014.5257868
CCTTGTT2886750.013.48802111
GCCAGTT2226250.012.9738283
CTTGTTT3057450.012.78713812
GTGCCAG2100000.012.6694051
TGCCAGT2250100.012.2685532
GATCGGA186000.011.9743721
CGAGACC179600.011.52332129
TAGTTAC322000.011.3273425
ATCGGAA206650.011.1530072
AATGCCG209200.010.9209224
AGACCGC198450.010.77756731
CGGTTAC159600.010.548723
Produced by FastQC (version 0.11.5)