FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053171
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1493342.4670375246296525RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC849001.4025706526380965RNA PCR Primer, Index 2 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA719851.1892114067155874RNA PCR Primer, Index 1 (100% over 28bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT632631.045121639550576RNA PCR Primer, Index 2 (100% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG615381.016624179293795RNA PCR Primer, Index 2 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC609061.006183370666383RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG600800.992537630276759RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT572600.9459504778569777RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT551270.9107127487394624RNA PCR Primer, Index 2 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG524410.8663393120729614RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA406540.6716149271183649RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG329550.5444253929056357RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC327500.541038738208453RNA PCR Primer, Index 2 (100% over 34bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC302220.49927550369880513No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA259250.428287917192493No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA253090.4181114328341294No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA234900.38806106749668895No Hit
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA211160.3488419540766319RNA PCR Primer, Index 2 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA204850.33841766571603543No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC204480.337806415843861No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG204470.3377898955770455No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC198160.32736560721644903RNA PCR Primer, Index 1 (100% over 31bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG196480.3245902023914408RNA PCR Primer, Index 2 (100% over 38bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC184130.30418767287426707RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA183670.3034277406007529RNA PCR Primer, Index 2 (100% over 36bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG180670.2984716605560953RNA PCR Primer, Index 2 (100% over 38bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG179920.2972326405449309RNA PCR Primer, Index 2 (100% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC173790.2871057169870139RNA PCR Primer, Index 1 (100% over 31bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC169480.2799854819895225RNA PCR Primer, Index 1 (100% over 24bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG163330.2698255178979745No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG163050.26936295042713976RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC162380.26825609255049954RNA PCR Primer, Index 1 (100% over 27bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC160240.26472075545197715RNA PCR Primer, Index 1 (100% over 26bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA160040.26439035011566664RNA PCR Primer, Index 2 (100% over 40bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT158410.26169754662473604RNA PCR Primer, Index 2 (100% over 37bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG155760.2573196759186218RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT147380.24347569232721164RNA PCR Primer, Index 2 (100% over 37bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA147280.24331048965905636RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC143420.23693366666826363RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC142910.23609113306067184RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC140910.2327870796975668RNA PCR Primer, Index 1 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG139980.23125069488372293No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG137560.22725279031436582RNA PCR Primer, Index 2 (100% over 35bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC136180.22497299349382333RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133170.22000039318235018RNA PCR Primer, Index 1 (100% over 30bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC131800.21773711662862327RNA PCR Primer, Index 2 (100% over 34bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG131020.2164485358170123RNA PCR Primer, Index 2 (100% over 35bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT127720.21099684776788893RNA PCR Primer, Index 2 (100% over 39bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC126390.20879965228142405RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG123390.20384357223676647RNA PCR Primer, Index 2 (100% over 35bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG123080.2033314439654852RNA PCR Primer, Index 2 (100% over 35bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA122520.20240630902381576RNA PCR Primer, Index 1 (100% over 32bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA121240.20029171487142855RNA PCR Primer, Index 1 (100% over 23bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC119980.19821016125267238RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT119200.1969215804410614RNA PCR Primer, Index 1 (100% over 30bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA118410.1956164793626349RNA PCR Primer, Index 1 (100% over 32bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT116300.19213070306455907RNA PCR Primer, Index 1 (100% over 25bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC115530.19085864251976362RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA112620.18605124487644575RNA PCR Primer, Index 2 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT111930.18491134646617452RNA PCR Primer, Index 2 (100% over 39bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA109360.1806656378945845RNA PCR Primer, Index 2 (100% over 36bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG107960.17835280054041097RNA PCR Primer, Index 2 (100% over 38bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT105120.1736610447648018No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA104330.17235594368637527RNA PCR Primer, Index 2 (100% over 36bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC104250.17222378155185109RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA103170.17043959273577436RNA PCR Primer, Index 1 (100% over 32bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCGTC101720.16804415404752318RNA PCR Primer, Index 2 (100% over 51bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG100460.165962600428767RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG100140.1654339518906702Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC99830.16492182361938892RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA95110.157124257682461RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG94090.1554391904672774RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC93190.15395236645388014RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC91160.15059875229032849RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTCT89870.14846763787112574No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT88200.145708753312933RNA PCR Primer, Index 1 (100% over 25bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC87190.14404020636456494RNA PCR Primer, Index 1 (100% over 33bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT85620.1414465244745275RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA85270.14086831513598408RNA PCR Primer, Index 1 (100% over 32bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC84910.1402735855306252RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG84240.139166727653985Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG84230.1391502073871695RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC83390.13776250497466533RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA81660.13490449881557948RNA PCR Primer, Index 1 (100% over 28bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC80040.1322282155914644RNA PCR Primer, Index 2 (100% over 34bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG79890.1319804115892315No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG79810.1318482494547073RNA PCR Primer, Index 1 (100% over 29bp)
CAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGG77250.12761906114993282No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT77170.1274868990154086RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC76880.1270078112777584No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCTC76130.125768791266594No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA76050.1256366291320698RNA PCR Primer, Index 2 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC75450.12464541312313826RNA PCR Primer, Index 1 (100% over 31bp)
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTG74160.1225142987039355No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG73840.1219856501658387RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG73720.1217874069640524RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCT73600.12158916376226608RNA PCR Primer, Index 2 (100% over 51bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT72910.12044926535199484RNA PCR Primer, Index 2 (100% over 39bp)
CTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATT71890.11876419813681127No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC71450.11803730639692814RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG70560.1165670026503464Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT70430.11635223918174459RNA PCR Primer, Index 2 (100% over 37bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA69760.1152453813051044No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA69070.11410548289483313RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG68680.11346119248902765No Hit
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC68160.11260213861462035No Hit
TTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA65490.10819122737487509RNA PCR Primer, Index 1 (100% over 32bp)
ATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG61860.1021943705208394RNA PCR Primer, Index 2 (100% over 38bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG61850.10217785025402389RNA PCR Primer, Index 2 (100% over 35bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA61490.10158312064866497RNA PCR Primer, Index 1 (100% over 32bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC61350.10135183691324763RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACA7050.045.0200231
CGTACAA253.8886392E-545.008122
TTTTCGC207.0351467E-445.00068724
AACACGC207.0351467E-445.00068722
GCTATAC1350.044.9977158
GTGTGCA551.8189894E-1244.99771510
GAAGGTA207.037437E-444.99771510
TCCTTCA87050.044.890731
ATCTAAA11050.044.816311
GTCTACG212300.044.7761541
CAACGCT15050.044.6987237
TGAGGTA32900.044.6779251
TTCAACG15550.044.565865
AGCTTAT43550.044.4913832
TTCACAG31600.044.450151
GCTACAT7150.044.3786352
CCTTCAT88050.044.3691672
CTTCATT90850.044.3367543
TCTTTCA3200.044.3165861
TCAAGTA9200.044.2742962