FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928792
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC509952.6438828033297526RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA401462.08140639322436No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC230691.1960335795669RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA213771.108310279179922No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA176950.9174135935860374RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC172890.8963641491669397No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA164020.8503768161626553RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA161270.8361191875536605RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG146750.7608389084981687RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT121650.6307056437397086RNA PCR Primer, Index 1 (100% over 30bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA110680.5738306670703736RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC108970.5649650143716896RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG98460.5104749501242228RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT96780.5017648351921824RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGTATGCC96430.4999502279146741RNA PCR Primer, Index 46 (100% over 51bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG89560.4643320793532947RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC84410.43763142941281385RNA PCR Primer, Index 5 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA79940.41445630218292073No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC79900.41424891849406265RNA PCR Primer, Index 46 (100% over 37bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC78620.4076126404506033No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG78170.4052795739509496RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA76770.39802114484091594RNA PCR Primer, Index 1 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA71850.3725129511113692No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC70660.3663432863678406RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC68650.35592225600272087RNA PCR Primer, Index 1 (100% over 31bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA64390.3338358931393328No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT63160.3274588447069461RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG58750.30459479301034015No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC56690.2939145330341478No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG56550.2931886901231444RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT55400.28722640906847396RNA PCR Primer, Index 3 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC54990.28510072625767835No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG53910.27950136665850955RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA53400.2768572246255688RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCG53200.27582030618127823RNA PCR Primer, Index 46 (100% over 38bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC51570.26736942086031046No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG50750.26311805523871934No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA48890.2534747137068175RNA PCR Primer, Index 1 (100% over 23bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC48450.2511934931293784RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT47580.24668289789671463RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC47470.24611259275235486No Hit
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT46620.24170568936412012RNA PCR Primer, Index 3 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC45880.2378690911202452RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC44940.23299557443207974RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA44530.2308698916212842No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC44090.22858867104384506RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC43840.2272925229884819No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA42260.21910086727858682No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG42060.21806394883429628RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC41740.21640487932343147RNA PCR Primer, Index 1 (100% over 33bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC39850.20660600002488605RNA PCR Primer, Index 5 (97% over 35bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA39700.20582831119166814No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG39540.20499877643623574Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC38500.19960680052592503No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC38240.19825880654834738RNA PCR Primer, Index 46 (100% over 37bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC37490.1943703623822579RNA PCR Primer, Index 46 (100% over 37bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAA36650.19001530491623772RNA PCR Primer, Index 46 (100% over 40bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCC36520.18934130792744888RNA PCR Primer, Index 6 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA36390.18866731093866004No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36010.18669716589450805RNA PCR Primer, Index 1 (100% over 33bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGA35960.18643793628343544RNA PCR Primer, Index 46 (100% over 39bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC34660.17969796639554705RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC34640.179594274551118RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG34600.1793868908622599No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG34140.1770019784403917Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC32900.1705730840857905RNA PCR Primer, Index 1 (100% over 33bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA32680.1694324737970709No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG32220.1670475613752027No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC32190.16689202360855915No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC31940.165595875553196RNA PCR Primer, Index 1 (100% over 24bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCG30820.15978913226516908RNA PCR Primer, Index 46 (100% over 38bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCC30120.15615991771015225RNA PCR Primer, Index 6 (97% over 36bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT29940.1552266911102908RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT29700.15398238897714217RNA PCR Primer, Index 3 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC29690.15393054305492765RNA PCR Primer, Index 1 (100% over 27bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC29590.15341208383278238RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC29450.15268624092177902RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA27930.14480566074517107RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAAT27540.14278366977880455RNA PCR Primer, Index 46 (100% over 41bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA27490.14252444016773191RNA PCR Primer, Index 1 (100% over 32bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT27490.14252444016773191RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC27420.14216151871223023RNA PCR Primer, Index 5 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA26930.13962106852371847No Hit
GTTTTCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA26750.138687841923857No Hit
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA26400.1368732346463486RNA PCR Primer, Index 1 (100% over 32bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG26250.1360955458131307RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC25250.13091095359167812RNA PCR Primer, Index 5 (97% over 35bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC24670.1279038901032356RNA PCR Primer, Index 5 (97% over 35bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT24470.1268669716589451RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG24330.12614112874794173No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA23800.12339329487057185RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23620.12246006827071038RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG23280.12069730691541648RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA23190.12023069361548576RNA PCR Primer, Index 1 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG22910.11877900779347904No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT22430.11629040352718178RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA22380.11603117391610915No Hit
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC22340.11582379022725105RNA PCR Primer, Index 46 (100% over 37bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC22310.11566825246060747RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG22010.11411287479417169RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT22000.11406102887195717RNA PCR Primer, Index 3 (100% over 34bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATC21990.11400918294974265RNA PCR Primer, Index 46 (100% over 42bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC21230.11006889286143866RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT21050.10913566626157718RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC20920.10846166927278836RNA PCR Primer, Index 46 (100% over 37bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT20300.10524722209548774RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC20240.1049361465622006RNA PCR Primer, Index 5 (97% over 35bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG20190.10467691695112798RNA PCR Primer, Index 1 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT20040.10389922811791007RNA PCR Primer, Index 1 (100% over 30bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG19600.10161800754047091No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCC19340.10027001356289326RNA PCR Primer, Index 6 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA19310.10011447579624969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATT1500.045.240351
TACCTTA302.0882671E-645.240351
AGCTACA1400.045.2403451
TCCTTCA11450.045.0427931
GTCTACG61550.045.0198441
GCTATTA253.8936334E-544.996418
AACGTGC351.2135206E-744.99522822
CCAGGTA351.2135206E-744.99522814
ACCACGG207.0381537E-444.99522814
GCCAGTA700.044.99522816
GTGCCCG253.8942297E-544.99522825
CAACGCT2650.044.994077
AACCGAC2000.044.99406410
CTCACAA453.8562575E-1044.9940648
TTAATGA207.0390524E-444.9940643
CGTATAA253.894826E-544.9940649
TCTCTAG351.213757E-744.9940644
CTATACC453.8562575E-1044.9940649
CAGCACG1050.044.9940644
GCTATCT207.0390524E-444.9940646
Produced by FastQC (version 0.11.5)