FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6115948
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC152696724.966971596226784RNA PCR Primer, Index 13 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1538672.5158323779077256RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1005891.6447000530416545RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA558770.9136277810079484RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC470620.7694964051362111RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTC460990.7537506859116526RNA PCR Primer, Index 13 (100% over 51bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG456120.7457878974772186RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT378370.618661244340207RNA PCR Primer, Index 13 (100% over 46bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG366880.5998742958573225RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC338590.5536181798798813RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA283300.46321518757190216RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA270090.44161591955981316RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC244530.3998235433002374RNA PCR Primer, Index 13 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG228880.3742347057234627RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC215060.3516380453202022RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG208180.3403887672033837RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT201770.32990797174861525RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA195680.3199503985318384RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA193700.31671296093426565RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG186130.3043354848667778RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC184640.3018992313211296RNA PCR Primer, Index 1 (100% over 31bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC174220.2848618071965295RNA PCR Primer, Index 13 (100% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCGTATGCCG172460.28198408488757587RNA PCR Primer, Index 13 (97% over 39bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA171890.28105209527615344RNA PCR Primer, Index 13 (100% over 40bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA166760.27266418877335125RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC165230.2701625324479541No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT161390.2638838655920554RNA PCR Primer, Index 8 (97% over 36bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT154370.25240567774611555RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC153960.25173529925368887RNA PCR Primer, Index 13 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG153160.25042724365871RNA PCR Primer, Index 1 (100% over 29bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC150050.2453421775332295RNA PCR Primer, Index 1 (100% over 33bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG148300.24248080591921317RNA PCR Primer, Index 2 (97% over 35bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC148190.2423009482749036RNA PCR Primer, Index 13 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG146570.23965213569507132RNA PCR Primer, Index 13 (100% over 45bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT145360.2376737016076657RNA PCR Primer, Index 1 (100% over 30bp)
CTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCT143460.23456706956959086RNA PCR Primer, Index 13 (100% over 51bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA143250.2342237049759089RNA PCR Primer, Index 13 (100% over 38bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT141860.23195095837963306RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA141110.23072465625934035RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT138060.22573769430348328RNA PCR Primer, Index 13 (100% over 41bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG135950.22228769767172643RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG132230.21620523915507456RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG131750.21542040579808722RNA PCR Primer, Index 13 (100% over 45bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA128450.21002467646879927RNA PCR Primer, Index 13 (100% over 38bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA127770.2089128292130672RNA PCR Primer, Index 13 (100% over 39bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG125690.20551188466612205RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA122860.20088463799888423RNA PCR Primer, Index 1 (100% over 34bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT119050.19465502322779724RNA PCR Primer, Index 13 (100% over 41bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC117620.19231687385177246RNA PCR Primer, Index 1 (100% over 33bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCT111620.18250645688943073RNA PCR Primer, Index 13 (100% over 51bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG110970.18144366171851037RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC110630.18088773809064432RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT109170.17850053662980783RNA PCR Primer, Index 1 (100% over 30bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT108220.1769472206107704RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA106120.1735135746739508RNA PCR Primer, Index 13 (100% over 40bp)
TCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTC105450.17241807811315596RNA PCR Primer, Index 13 (100% over 51bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC103860.1698183176181354RNA PCR Primer, Index 1 (100% over 26bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT101600.16612306056232RNA PCR Primer, Index 1 (100% over 25bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG101580.16609035917244555RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC99530.1627384667103121RNA PCR Primer, Index 13 (100% over 44bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA97080.15873254645068924RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC95110.1555114595480537RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG92810.1517507997124894RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG91190.1491019871326571RNA PCR Primer, Index 1 (100% over 29bp)
TGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT89350.14609345926420564RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC89340.14607710856926842RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC89050.14560293841608857RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT88300.14437663629579583RNA PCR Primer, Index 8 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA84600.13832687916901842RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG81090.13258778524604853RNA PCR Primer, Index 2 (97% over 35bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG78370.12814039622312026RNA PCR Primer, Index 13 (100% over 45bp)
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC77830.12725745869650953RNA PCR Primer, Index 13 (98% over 51bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG77790.12719205591676058RNA PCR Primer, Index 2 (97% over 35bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC76830.1256223892027859RNA PCR Primer, Index 1 (100% over 27bp)
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG75710.12379111136981544RNA PCR Primer, Index 1 (100% over 29bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA74950.12254845855458549RNA PCR Primer, Index 13 (100% over 39bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATG73580.12030841334818414RNA PCR Primer, Index 13 (100% over 49bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG73070.11947452790638508RNA PCR Primer, Index 2 (97% over 35bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA73010.11937642373676166RNA PCR Primer, Index 1 (100% over 32bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCG71800.11739798964935608RNA PCR Primer, Index 13 (100% over 51bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA68870.11260723603274587RNA PCR Primer, Index 13 (100% over 39bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC68500.11200226032006813RNA PCR Primer, Index 1 (100% over 27bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC67990.11116837487826908RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT67230.10992572206303913RNA PCR Primer, Index 13 (100% over 43bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC66390.10855226368831129RNA PCR Primer, Index 1 (100% over 33bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG65400.10693354488952489Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA65150.10652477751609399RNA PCR Primer, Index 1 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT64090.10479160385274695RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT63850.10439918717425327RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG63840.10438283647931604RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC62380.10199563501847955RNA PCR Primer, Index 13 (100% over 37bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG61730.1009328398475592RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT61200.10006625301588568RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG253.8847822E-545.0156971
CGACTAT253.8847822E-545.0156971
AGCTACA4550.045.0156971
TCACAAA207.023604E-445.0156941
GTTTACG700.045.0156941
TCCTCAT351.2097553E-745.0156941
CGACAAA207.023604E-445.0156941
TAGTTTA3250.045.0156941
AATGCCT207.023604E-445.0156941
AGTGCCG207.023604E-445.0156941
AAGGGGT207.023604E-445.0156941
TCTTTCA6300.045.0156941
GCAGCGA1100.045.0156941
AGCTATA302.1618507E-645.0156941
CATCCGT302.1618507E-645.0156941
CGTATCG253.8889117E-545.00762
CCATCGA253.8889117E-545.00762
ATCTTCC302.16453E-645.00762
AATTATT302.16453E-645.00762
TTAAGTA453.8562575E-1045.0075952
Produced by FastQC (version 0.11.5)