FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026032
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC1900434.720354930114812RNA PCR Primer, Index 11 (100% over 51bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT643681.5987950418675263RNA PCR Primer, Index 11 (100% over 37bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA454301.1284063315939863RNA PCR Primer, Index 11 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT430781.0699865276778724RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG407781.0128583180660262RNA PCR Primer, Index 6 (100% over 34bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA323900.8045142214468239RNA PCR Primer, Index 11 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC307800.7645244747185318RNA PCR Primer, Index 11 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG277430.6890903996788899RNA PCR Primer, Index 1 (100% over 29bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG244760.6079435036780632RNA PCR Primer, Index 2 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG229770.5707108140223426RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG223890.5561058630433141RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA222940.5537462196028248RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC209360.5200157375798304RNA PCR Primer, Index 1 (100% over 33bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT187410.4654955549285251RNA PCR Primer, Index 11 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA185540.460850783103562RNA PCR Primer, Index 1 (100% over 28bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA180620.448630313917028RNA PCR Primer, Index 11 (100% over 38bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA175060.43482019020216434RNA PCR Primer, Index 11 (100% over 40bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA168340.4181288176547032RNA PCR Primer, Index 11 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC161630.4014622834592472RNA PCR Primer, Index 1 (100% over 27bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA158210.3929675670734858RNA PCR Primer, Index 11 (100% over 38bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG147920.3674089028601859RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT146650.3642544321555318RNA PCR Primer, Index 11 (100% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC144810.35968417538658415RNA PCR Primer, Index 1 (100% over 31bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC143320.3559832609378167RNA PCR Primer, Index 11 (100% over 42bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT142310.3534745873852965RNA PCR Primer, Index 11 (100% over 41bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC139530.3465695255278647RNA PCR Primer, Index 11 (100% over 39bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA138520.3440608519753445RNA PCR Primer, Index 1 (100% over 32bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC138430.3438373068072981RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC135400.3363112861497375RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC135160.3357151657016139RNA PCR Primer, Index 6 (97% over 36bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC132390.3288349421961872RNA PCR Primer, Index 1 (100% over 33bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG123320.30630655692751574RNA PCR Primer, Index 6 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC122870.30518883108728395RNA PCR Primer, Index 1 (100% over 31bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT122140.30337563139090795RNA PCR Primer, Index 11 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG112730.28000274215406135RNA PCR Primer, Index 11 (100% over 45bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC111630.2772705234334948RNA PCR Primer, Index 6 (97% over 36bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG106270.26395716675873415RNA PCR Primer, Index 6 (100% over 34bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA104150.25869143613364226RNA PCR Primer, Index 1 (100% over 32bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA103010.2558598640050551RNA PCR Primer, Index 11 (100% over 38bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC96590.23991364201774848RNA PCR Primer, Index 11 (100% over 51bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG94330.23430017446458448RNA PCR Primer, Index 2 (97% over 35bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC85860.21326209031622204RNA PCR Primer, Index 1 (100% over 33bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC85470.21229339458802116RNA PCR Primer, Index 11 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT84480.20983439773951126RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG83210.20667992703485716RNA PCR Primer, Index 2 (97% over 35bp)
CACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC83090.20638186681079534RNA PCR Primer, Index 1 (100% over 33bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA81220.2017370949858322RNA PCR Primer, Index 11 (100% over 40bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT80440.1997997035294305RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG80040.1988061694492244RNA PCR Primer, Index 6 (100% over 34bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC79050.19634717260071455RNA PCR Primer, Index 1 (100% over 33bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT76560.19016242295143207RNA PCR Primer, Index 11 (100% over 37bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT74430.18487185397433503RNA PCR Primer, Index 11 (100% over 41bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG71950.1787119426770577RNA PCR Primer, Index 6 (100% over 34bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC71170.17677455122065597RNA PCR Primer, Index 11 (100% over 39bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA70060.17401749414808426RNA PCR Primer, Index 1 (100% over 28bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT69210.17190623422764648RNA PCR Primer, Index 11 (100% over 41bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC64990.16142444968147296RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG64350.15983479515314336RNA PCR Primer, Index 2 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG63960.15886609942494248RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT62360.1548919631041184RNA PCR Primer, Index 11 (100% over 46bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG62010.15402262078393814RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC58570.14547822769416635RNA PCR Primer, Index 1 (100% over 31bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG55860.1387470343007706RNA PCR Primer, Index 2 (97% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG55620.13815091385264697RNA PCR Primer, Index 2 (97% over 35bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT54920.13641222921228643RNA PCR Primer, Index 1 (100% over 25bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG54760.13601481558020403RNA PCR Primer, Index 1 (100% over 29bp)
CACTTGGACGAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT54560.13551804854010102RNA PCR Primer, Index 11 (100% over 37bp)
TCCTTCATTCCACCGGACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG54270.13479773633195166RNA PCR Primer, Index 1 (100% over 29bp)
TACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC54140.1344748377558847RNA PCR Primer, Index 11 (100% over 39bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA53610.13315840509961174RNA PCR Primer, Index 1 (100% over 32bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA53090.1318668107953439RNA PCR Primer, Index 11 (100% over 38bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA52340.1300039343949576RNA PCR Primer, Index 11 (100% over 38bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG52240.12975555087490612RNA PCR Primer, Index 6 (100% over 34bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT50160.12458917365783481RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA49490.12292500407348973RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC46640.11584607375202183RNA PCR Primer, Index 6 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA45020.11182226072718746RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC44670.1109529184070072RNA PCR Primer, Index 1 (100% over 33bp)
GTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC44340.11013325279083724RNA PCR Primer, Index 11 (100% over 39bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA42160.10471849205371442RNA PCR Primer, Index 11 (100% over 38bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT41830.10389882643754444RNA PCR Primer, Index 11 (100% over 41bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA41560.10322819093340539No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA41530.10315367587738994RNA PCR Primer, Index 1 (100% over 23bp)
GCACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA40350.10022275034078218RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAT351.2096461E-745.0155941
TGAGGTA4050.045.015591
CCTTTAT1900.045.0072062
TCAAGTA1900.045.0072062
ACCTTAT253.8888735E-545.0072062
ACCCGTA3850.045.0072022
GTTTATC253.8908707E-545.0032923
ATACTTA253.8908707E-545.0032923
TTGCATT302.1657706E-645.0032923
CAGCTAC207.0345786E-445.0010633
ACCCGCA302.1666965E-645.00049623
TACTTAA253.892583E-544.999944
ACATTGC302.166882E-644.999944
TATGAGC253.892583E-544.999945
ATTGCCA253.892583E-544.999946
CCGTTAC551.8189894E-1244.999944
AGTAATT750.044.999945
TAGCCTA207.037161E-444.99770715
TAGTATA207.037161E-444.9977078
GAAGCTC453.8562575E-1044.9977048
Produced by FastQC (version 0.11.5)