FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661244
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT116491.7616795010616353RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG99841.5098813751051048RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA90771.3727156692537097RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC78981.1944153746574637RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA67521.0211056735486448RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA64220.9711997386743774RNA PCR Primer, Index 39 (100% over 38bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC59690.9026925008015196RNA PCR Primer, Index 2 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC56180.8496107337079808RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG55860.8447713703262336RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT55570.8403856972615251RNA PCR Primer, Index 2 (97% over 37bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC40280.6091548656774202RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG37190.5624247630224244RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC36300.5489652836169402RNA PCR Primer, Index 2 (100% over 34bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT32800.4960347466290809RNA PCR Primer, Index 1 (97% over 35bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC32040.4845412585974315RNA PCR Primer, Index 39 (100% over 39bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC30790.46563749538748184RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT30770.46533503517612257RNA PCR Primer, Index 1 (100% over 30bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG27710.4190586228381656RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT26900.4068089842781182RNA PCR Primer, Index 2 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC25140.380192485678509RNA PCR Primer, Index 1 (100% over 31bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA25030.3785289545160334RNA PCR Primer, Index 39 (100% over 40bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC23020.34813170327443427RNA PCR Primer, Index 1 (100% over 24bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG21970.3322525421780765RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT21850.33043778090992126RNA PCR Primer, Index 39 (100% over 41bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC20950.3168270713987575RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC20690.31289508865108795RNA PCR Primer, Index 39 (100% over 51bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA19660.2973183877660894RNA PCR Primer, Index 39 (100% over 38bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA18530.2802293858242948RNA PCR Primer, Index 2 (97% over 36bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA18120.2740289514914313RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT17970.27176049990623735RNA PCR Primer, Index 2 (97% over 37bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA17520.26495514515065544RNA PCR Primer, Index 39 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG17260.2610231624029859RNA PCR Primer, Index 1 (100% over 29bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT16890.2554276484928408RNA PCR Primer, Index 39 (100% over 41bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA16820.2543690377530836RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG16600.25104197542813245RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG16590.2508907453224528RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA16570.2505882851110936RNA PCR Primer, Index 2 (97% over 36bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA16440.24862229373725886RNA PCR Primer, Index 39 (100% over 40bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA16330.2469587625747833RNA PCR Primer, Index 1 (100% over 23bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC16020.24227062929871576RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA15870.24000217771352178RNA PCR Primer, Index 1 (100% over 28bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC14850.22457670693420279RNA PCR Primer, Index 2 (100% over 34bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA14660.22170333492629044RNA PCR Primer, Index 39 (100% over 38bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC13920.21051230710600022RNA PCR Primer, Index 2 (100% over 34bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC13820.2090000060492042RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC13750.20794139530944705RNA PCR Primer, Index 1 (100% over 24bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC13690.20703401467536947No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT13640.20627786414697144RNA PCR Primer, Index 1 (100% over 30bp)
CACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC13380.20234588139930193RNA PCR Primer, Index 1 (100% over 33bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT13020.1969015975948364RNA PCR Primer, Index 2 (97% over 37bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC12590.19039870305061368RNA PCR Primer, Index 1 (100% over 33bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT12540.1896425525222157RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA12040.18208104723823582No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT11980.18117366660415823RNA PCR Primer, Index 1 (97% over 35bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA11900.17996382575872144RNA PCR Primer, Index 39 (100% over 40bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATC11820.17875398491328465RNA PCR Primer, Index 39 (100% over 42bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA11670.1764855333280907RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11630.1758806129053723RNA PCR Primer, Index 1 (100% over 33bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11620.1757293827996927RNA PCR Primer, Index 1 (100% over 33bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT11490.17376339142585795RNA PCR Primer, Index 39 (100% over 41bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT11110.16801664741003322RNA PCR Primer, Index 39 (100% over 41bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT10810.16347974423964526RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG10620.16060637223173294Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATC10380.15697684969542255RNA PCR Primer, Index 39 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTC10280.15546454863862658RNA PCR Primer, Index 39 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA10100.15274240673639383No Hit
TCGTGGTTGTAGCCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA9990.15107887557391825RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG9980.15092764546823864No Hit
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA9520.14397106060697715RNA PCR Primer, Index 39 (100% over 38bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT9490.14351737028993836RNA PCR Primer, Index 1 (97% over 35bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA9330.1410976885990648RNA PCR Primer, Index 39 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA9270.14019030796498722No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA9260.1400390778593076RNA PCR Primer, Index 1 (100% over 22bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC9240.13973661764794842RNA PCR Primer, Index 39 (100% over 39bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC9160.13852677680251163RNA PCR Primer, Index 39 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9140.13822431659115245RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC9110.13777062627411363RNA PCR Primer, Index 39 (100% over 39bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC9060.13701447574571565RNA PCR Primer, Index 2 (100% over 34bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT9010.13625832521731765RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC8770.13262880268100732RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC8720.13187265215260932No Hit
GTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC8360.1264283683481438RNA PCR Primer, Index 39 (100% over 39bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG8160.12340376623455183RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG8070.12204269528343545RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG8060.12189146517775587RNA PCR Primer, Index 1 (100% over 29bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA7990.12083285443799868No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC7940.1200767039096007RNA PCR Primer, Index 39 (100% over 39bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC7910.1196230135925619RNA PCR Primer, Index 1 (100% over 27bp)
CTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC7130.10782706534955327RNA PCR Primer, Index 39 (100% over 39bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA7050.10661722450411648RNA PCR Primer, Index 1 (100% over 23bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT6980.1055586137643593RNA PCR Primer, Index 1 (97% over 35bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC6980.1055586137643593RNA PCR Primer, Index 39 (100% over 39bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT6870.10389508260188372RNA PCR Primer, Index 1 (100% over 30bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA6800.10283647186212652RNA PCR Primer, Index 39 (100% over 40bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG6760.10223155143940814No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA6670.10087048048829177RNA PCR Primer, Index 2 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA6650.10056802027693257No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG6640.10041679017125298RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph