FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6003632
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2761324.599415820290117RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1173311.9543336433678813RNA PCR Primer, Index 2 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC953771.588655000839492RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA828841.3805642984113617RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA598270.9965134438619822RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG572930.954305660306961RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA509150.8480699683125148RNA PCR Primer, Index 22 (100% over 37bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA479870.7992994907082912RNA PCR Primer, Index 22 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT477590.7955017895833721RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC399720.6657969709002817No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG370910.6178093527384756RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC357280.5951064289083674RNA PCR Primer, Index 22 (100% over 51bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG286740.4776108862102141RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC280850.4678001583041732RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG270770.45101032175189953RNA PCR Primer, Index 22 (100% over 39bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC232220.3867991908897814RNA PCR Primer, Index 22 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG201200.33513046769022486RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA198050.32988364376763935RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG195670.32591937680390803RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC193860.3229045351214065RNA PCR Primer, Index 22 (100% over 38bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC193000.32147206890762126RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG179800.2994853781844057RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC175140.29172341009575536No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC166970.27811498106479543RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTC164350.2737509560879148RNA PCR Primer, Index 22 (100% over 51bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG164110.27335119807476543RNA PCR Primer, Index 2 (100% over 35bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA156280.2603090928957671RNA PCR Primer, Index 22 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA153480.2556452494090244No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG152370.2537963685982086RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC151970.2531301052429596RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA143390.2388387562728695RNA PCR Primer, Index 1 (100% over 23bp)
CTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCT142770.2378060480722336RNA PCR Primer, Index 22 (100% over 51bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA140530.23407497328283944No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA138080.22999411023193958RNA PCR Primer, Index 22 (100% over 37bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA136540.22742899631423114RNA PCR Primer, Index 22 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA135400.2255301457517716RNA PCR Primer, Index 1 (100% over 28bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134710.22438084146396714RNA PCR Primer, Index 1 (100% over 31bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC134330.22374789127648062RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC129700.21603589293947398RNA PCR Primer, Index 2 (100% over 34bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC129330.21541959933586868RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC128600.21420366871253935RNA PCR Primer, Index 2 (100% over 34bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA126980.21150530212378105RNA PCR Primer, Index 1 (100% over 22bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT126520.21073909926524478RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC123740.20610856894626453RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC121330.20209433223088957RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC120980.20151135179504676RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT120300.20037870409112352RNA PCR Primer, Index 12 (97% over 36bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC114820.1912508961242128RNA PCR Primer, Index 1 (100% over 33bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG110290.1837054636260184RNA PCR Primer, Index 2 (100% over 35bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA107830.1796079439912373RNA PCR Primer, Index 22 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA104390.17387807913609626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA104350.1738114528005714No Hit
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC104250.17364488696175914RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC103510.17241229975454858RNA PCR Primer, Index 1 (100% over 31bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG101260.1686645683812732RNA PCR Primer, Index 22 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA100020.16659915198000144No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT97570.16251828892910158RNA PCR Primer, Index 1 (100% over 30bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA97230.16195196507713996RNA PCR Primer, Index 22 (100% over 40bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT95220.15860399171701398RNA PCR Primer, Index 12 (97% over 36bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG93630.15595559487989935Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG91760.15284081369411048No Hit
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC90420.1506088314540265RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC88100.14674450399358255RNA PCR Primer, Index 1 (100% over 33bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG87120.14511215877322262No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC86690.14439592566632997RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA86540.14414607690811163No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT85170.14186412491638395RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC84550.14083141671574806No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTC82310.13710034192635392RNA PCR Primer, Index 22 (100% over 51bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG78780.13122056781628189RNA PCR Primer, Index 1 (100% over 29bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA78720.13112062831299454RNA PCR Primer, Index 1 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG78510.13077084005148884Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC78210.13027114253505212RNA PCR Primer, Index 2 (100% over 34bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA78090.13007126352847742RNA PCR Primer, Index 22 (100% over 40bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA77780.1295549094281595RNA PCR Primer, Index 1 (100% over 32bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG77760.12952159626039705Illumina Small RNA Adapter 2 (100% over 21bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC77590.12923843433441623RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG76620.12762274569793752No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC76540.12748949302688772RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC76260.12702310867821345No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC76120.1267899165038763RNA PCR Primer, Index 22 (100% over 38bp)
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT75990.1265733809134204RNA PCR Primer, Index 22 (100% over 41bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA75980.12655672432953918RNA PCR Primer, Index 1 (100% over 28bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG75840.12632353215520206RNA PCR Primer, Index 2 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC75780.1262235926519147RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC74080.12339197339210665RNA PCR Primer, Index 1 (100% over 27bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA73980.12322540755329439RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC73720.1227923363723826RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA73330.12214272960101484No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC72570.12087682922604184RNA PCR Primer, Index 1 (100% over 24bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC72040.11999403028033696RNA PCR Primer, Index 22 (100% over 38bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC71110.11844496797938314RNA PCR Primer, Index 1 (100% over 31bp)
CAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC70150.11684593592678565RNA PCR Primer, Index 22 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC69120.1151303077870196No Hit
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG68680.11439741809624573Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG68230.11364787182159067RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC68230.11364787182159067No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG67760.11286501237917314RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG67420.11229868852721152RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG67090.11174902125913114RNA PCR Primer, Index 1 (100% over 29bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA66880.11139923299762543RNA PCR Primer, Index 22 (100% over 37bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA66410.11061637355520791RNA PCR Primer, Index 1 (100% over 32bp)
ATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC66050.11001673653548386RNA PCR Primer, Index 22 (100% over 38bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT65200.10860092690557982RNA PCR Primer, Index 12 (97% over 36bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCG64600.1076015318727064RNA PCR Primer, Index 22 (100% over 51bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT63400.10560274180695951RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT61250.10202157627249638RNA PCR Primer, Index 1 (100% over 30bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT60710.10112212074291028RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGT206.831559E-445.2697261
GTCTACG84850.045.0296361
ACTCACA1950.044.99336216
GACACGA302.169616E-644.992244
GCTATAC2250.044.992248
CGACCTA351.2147211E-744.992243
ACATACC700.044.992249
CACATAA253.896749E-544.9922377
GGTCAAC207.0416526E-444.9922377
TTGCGCA406.833943E-944.9922378
GACATAC650.044.9922378
GGCATAG551.8189894E-1244.99223713
GACCTTC207.0416526E-444.9922377
TGAGGTA47850.044.93861
TTCACAG32800.044.855671
TCCTTCA42000.044.7846951
TTCAACG15850.044.7083785
GCTTATC61250.044.6984143
AGCTTAT63700.044.639082
CAACGGA6750.044.599061
Produced by FastQC (version 0.11.5)