FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5289216
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC4933819.328055424471227RNA PCR Primer, Index 9 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC998261.8873496563573882RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG886571.6761841452495037RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC595221.125346365132375RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA548141.0363350636464839RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG356110.6732755856444509RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG352730.6668852245777068RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC349470.6607217402352258RNA PCR Primer, Index 9 (100% over 37bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA342670.6478653925269832RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA324430.6133801304389913RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT312410.5906546452253038RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC290830.5498546476453221No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA271840.5139514060306858RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC262210.49574454890857167RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC242540.45855567252311114RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA237070.4482138751754513RNA PCR Primer, Index 9 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA228270.4315762487294903No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG228010.431084682493587RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG227540.4301960819902232RNA PCR Primer, Index 6 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG192980.36485558540244906RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA172380.32590841440394946RNA PCR Primer, Index 7 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG165800.3134680073568559RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC160250.30297495885968734RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT159680.3018972944194376RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG158190.2990802417598374RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA157730.29821054765016214RNA PCR Primer, Index 9 (100% over 40bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA157260.2973219471467983RNA PCR Primer, Index 1 (100% over 22bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG151690.28679108586225255RNA PCR Primer, Index 9 (100% over 39bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA151150.2857701406030686RNA PCR Primer, Index 9 (100% over 40bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG142510.26943501645612505RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC139100.2629879362083152RNA PCR Primer, Index 9 (100% over 37bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC136700.2584504017230531RNA PCR Primer, Index 9 (100% over 44bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG136160.2574294564638691RNA PCR Primer, Index 9 (100% over 51bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC130700.24710656550989787RNA PCR Primer, Index 9 (100% over 37bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC128560.2430605972605392RNA PCR Primer, Index 9 (100% over 42bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG126370.23892009704273753RNA PCR Primer, Index 6 (100% over 34bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC125910.2380504029330623RNA PCR Primer, Index 9 (100% over 37bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT125150.2366135170127293RNA PCR Primer, Index 1 (100% over 25bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC120440.22770860558540243RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG118860.2247213953826049RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT117980.2230576327380088RNA PCR Primer, Index 9 (100% over 41bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA113970.2154761688688834RNA PCR Primer, Index 9 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA113130.21388803179904167No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC111920.21160035816272205RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111620.2110331663520643RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT110640.20918033977058223RNA PCR Primer, Index 9 (100% over 36bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC110370.20866986714099026No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC108700.2055124993949954RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG108600.20532343545810947RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG106840.20199591016891727RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT106220.2008237137602246RNA PCR Primer, Index 1 (100% over 30bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA105280.19904651275349694RNA PCR Primer, Index 7 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG104300.19719368617201488RNA PCR Primer, Index 1 (100% over 29bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT103420.1955299235274188RNA PCR Primer, Index 9 (100% over 41bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT102230.19328006267847636RNA PCR Primer, Index 9 (100% over 41bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG98320.18588766274623686RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT96990.18337311238565412RNA PCR Primer, Index 9 (100% over 36bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG96860.18312732926770242RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG96530.1825034182759789RNA PCR Primer, Index 1 (100% over 29bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG94850.17932714413629544RNA PCR Primer, Index 9 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA94550.17875995232563768No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA94110.17792807100333963No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA93430.17664243623251538RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC92800.17545133343013405RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG90620.17132973960602102RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG89800.16977941532355645Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA89150.168550499733798RNA PCR Primer, Index 1 (100% over 23bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT88960.16819127825371474RNA PCR Primer, Index 1 (100% over 30bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA88590.16749174168723682RNA PCR Primer, Index 9 (100% over 40bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG87670.16575235346788636RNA PCR Primer, Index 9 (100% over 39bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA87490.16541203838149168RNA PCR Primer, Index 9 (100% over 38bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC84420.15960777551909394RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC83980.1587758941967959RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC82450.1558832159624413RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC81570.1542194533178452RNA PCR Primer, Index 1 (100% over 27bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC81100.1533308528144814RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA80780.15272584821644644RNA PCR Primer, Index 1 (100% over 28bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA79260.14985207637578046RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG78320.1480748753690528No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT75950.14359406006485648RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG72970.13795995474565606No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC72600.13726041817917817RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC72200.13650416243163446RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG71180.13457571027539808RNA PCR Primer, Index 9 (100% over 45bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG71150.13451899109433232RNA PCR Primer, Index 6 (100% over 34bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC69600.13158850007260056RNA PCR Primer, Index 9 (100% over 37bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA68940.13034067808915348RNA PCR Primer, Index 7 (97% over 35bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA68790.13005708218382458No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG68440.12939535840472388No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA68330.12918738807414937RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA67790.1281664428149654RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA67580.12776940854750496RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT67450.12752362542955326RNA PCR Primer, Index 9 (100% over 46bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA67420.1274669062484875RNA PCR Primer, Index 9 (100% over 38bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA66910.1265026801703693RNA PCR Primer, Index 1 (100% over 28bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA66420.12557626687962828RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA65770.1243473512898698No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG65420.12368562751076909RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT65090.12306171651904556RNA PCR Primer, Index 9 (100% over 43bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC64270.12151139223658099RNA PCR Primer, Index 9 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC63690.12041482140264265No Hit
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG63400.1198665359856735RNA PCR Primer, Index 9 (100% over 39bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG62840.11880777793911233RNA PCR Primer, Index 6 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA62840.11880777793911233RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG62630.11841074367165191Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA62350.11788136464837133RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC62260.11771120710517399RNA PCR Primer, Index 1 (100% over 24bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG61680.11661463627123565Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT59920.11328711098204346RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA59800.11306023425778036RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC57880.10943020666957068RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA56710.10721815860800542RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG56430.10668877958472485RNA PCR Primer, Index 9 (100% over 39bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG56160.10617830695513286RNA PCR Primer, Index 1 (100% over 29bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA55930.10574345990029524RNA PCR Primer, Index 9 (100% over 40bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT55590.10510064251488313RNA PCR Primer, Index 1 (100% over 25bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG55380.10470360824742268RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT53090.10037404409273509RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph