FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695653
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2811067.606395946805613RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC1716094.643536609091817RNA PCR Primer, Index 43 (100% over 51bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTC989052.6762523429553586RNA PCR Primer, Index 43 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA932152.522287671488638RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG786952.129393641664951RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA544861.4743267292681428RNA PCR Primer, Index 7 (97% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG455961.2337738418623176RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCT417491.1296785710130253RNA PCR Primer, Index 43 (100% over 51bp)
TCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTC353530.9566103744047397RNA PCR Primer, Index 43 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG304110.8228856984137851RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC268610.7268268963563408RNA PCR Primer, Index 1 (100% over 31bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCT241280.6528751481808492RNA PCR Primer, Index 43 (100% over 51bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC227410.6153445683347436RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC225940.611366922165041RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG201680.5457222309562072RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA186730.5052692988221568No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG174390.47187871805063947RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT167260.4525857811867077RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG162100.4386234313665271RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT158380.4285575512636062RNA PCR Primer, Index 1 (100% over 30bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT132420.35831286108300753RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT120480.3260046330107291RNA PCR Primer, Index 1 (100% over 30bp)
TTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTT114220.3090658132676417RNA PCR Primer, Index 43 (100% over 51bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTC106240.2874728769178275RNA PCR Primer, Index 43 (100% over 44bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC105300.2849293480746163RNA PCR Primer, Index 43 (100% over 42bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC102440.27719052627505886RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG101980.2759458206709342RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA101790.2754317031387958No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG101740.2752964090513909RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA97750.26449994087648376RNA PCR Primer, Index 43 (100% over 40bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG95470.2583305304908226Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT95360.25803288349853193RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG94840.25662582498952147No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC93470.25291876699462856RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA91730.24821053275293972RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG91390.24729053295858675RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGT90140.2439081807734655RNA PCR Primer, Index 43 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG88420.2392540641667386RNA PCR Primer, Index 43 (100% over 45bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT87930.23792818211017105RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA81990.22185524452647473No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA79070.21395406982203147RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC78970.21368348164722173RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA74980.2028870134723146No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC73920.2000187788193318RNA PCR Primer, Index 1 (100% over 27bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT69260.18740936987319967RNA PCR Primer, Index 3 (100% over 34bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG69090.18694936997602318Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA68180.18448701758525488RNA PCR Primer, Index 7 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG67740.18329642961609222RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA67600.1829176061713586RNA PCR Primer, Index 1 (100% over 28bp)
TCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCTG66730.1805634890505142RNA PCR Primer, Index 43 (100% over 51bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG64610.17482701974454853RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA64530.17461054920470076No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT64310.17401525522011943RNA PCR Primer, Index 3 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA63180.1709576088447698RNA PCR Primer, Index 43 (100% over 37bp)
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT62700.16965878560568323RNA PCR Primer, Index 43 (100% over 41bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG62500.1691176092560638RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA60890.1647611396416276RNA PCR Primer, Index 43 (100% over 37bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC60760.164409375014375RNA PCR Primer, Index 1 (100% over 27bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGC60560.16386819866475558RNA PCR Primer, Index 43 (100% over 39bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA59850.1619470226236067RNA PCR Primer, Index 43 (100% over 40bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG59210.1602152583048246RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC58550.1584293763510806No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG58270.1576717294616134RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG56600.1531529069422914RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCG56270.15225996596541938RNA PCR Primer, Index 43 (100% over 51bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG55490.1501493782019037RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT55040.14893173141526003RNA PCR Primer, Index 43 (100% over 41bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG54320.14698349655663018RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG53560.14492702642807645Illumina Small RNA Adapter 2 (100% over 21bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAG53380.14443996771341897RNA PCR Primer, Index 43 (100% over 38bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT52830.14295173275196563RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA52640.14243761521982717No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG52440.14189643887020778RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC52420.14184232123524584RNA PCR Primer, Index 1 (100% over 31bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATG51980.14065173326608316RNA PCR Primer, Index 43 (100% over 49bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51670.13981290992417308RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC50380.13632232246912793RNA PCR Primer, Index 1 (100% over 33bp)
ACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC50170.13575408730202754RNA PCR Primer, Index 43 (100% over 42bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA50120.1356187932146227No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG49790.1347258522377507RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA48660.13166820586240105RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA48300.1306940884330861RNA PCR Primer, Index 1 (100% over 28bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA47930.12969291218629023RNA PCR Primer, Index 7 (97% over 35bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC46450.12568820719910662RNA PCR Primer, Index 43 (100% over 50bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAC46280.12522820730193013RNA PCR Primer, Index 30 (97% over 36bp)
TCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGG45530.12319879599085735Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT44320.11992467907565997RNA PCR Primer, Index 3 (100% over 34bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA44080.11927526745611669RNA PCR Primer, Index 1 (100% over 32bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT43650.11811173830443497RNA PCR Primer, Index 43 (100% over 41bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG43200.11689409151779132RNA PCR Primer, Index 43 (100% over 45bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC40970.11085997521953495RNA PCR Primer, Index 1 (100% over 26bp)
TCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC40670.11004821069510586RNA PCR Primer, Index 43 (100% over 42bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC40640.10996703424266295RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC40320.10910115208327188RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG40080.1084517404637286No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT39940.10807291701899502RNA PCR Primer, Index 3 (100% over 34bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG37410.10122703619630956Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA37370.10111880092638567No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA37220.10071291866417112No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA37140.10049644812432336RNA PCR Primer, Index 1 (100% over 28bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTAT37060.1002799775844756RNA PCR Primer, Index 43 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCG302.1625146E-645.012981
TCTTACG207.025329E-445.012981
GAAAAAG253.8858656E-545.012981
AGCTATA207.025329E-445.012981
CATCCGT207.025329E-445.012981
ATCCCCC351.2101555E-745.0129781
TTAGTGC351.2101555E-745.0129781
GCAGCGA900.045.0129781
CGCACGA253.888351E-545.0081062
ATATGGT253.8905266E-545.0038455
TGCATGA600.045.0038455
GCAATGT253.8905266E-545.0038455
GACCTTA253.8905266E-545.0038454
TTATTCC1150.045.0038455
GATTAGC600.045.0038455
CGATAGC302.1655396E-645.0038455
AATACAA551.8189894E-1245.0038455
ACCTTAG253.8905266E-545.0038455
AGCACAG351.2120836E-745.003845
GGATGTA5150.045.003843
Produced by FastQC (version 0.11.5)