FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048200
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC818453.9959476613611953RNA PCR Primer, Index 40 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC767883.7490479445366662RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA324871.5861244019138756RNA PCR Primer, Index 1 (97% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT251971.2302021286983693RNA PCR Primer, Index 1 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC234341.1441265501415876RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTC227051.108534322820037RNA PCR Primer, Index 40 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC215461.051948051948052No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC213321.0414998535299287RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG203380.9929694365784592RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA144270.7043745727956254RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC119680.5843179377013964RNA PCR Primer, Index 1 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA112000.5468215994531784RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA110350.5387657455326629RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG106590.5204081632653061RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA101460.4953617810760668No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG100030.48838004101161997RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC98750.4821306513035836RNA PCR Primer, Index 2 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT97500.47602773166682943RNA PCR Primer, Index 1 (97% over 35bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG82140.4010350551703935RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG76830.3751098525534616RNA PCR Primer, Index 40 (100% over 45bp)
CTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTCT75290.3675910555609804RNA PCR Primer, Index 40 (100% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG70730.34532760472610097RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA66160.3230153305341275RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA61780.3016307001269407No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC58140.28385899814471244RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGT58010.28322429450249RNA PCR Primer, Index 40 (100% over 46bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC57910.2827360609315497No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTC57270.2796113660775315RNA PCR Primer, Index 40 (100% over 51bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA57190.2792207792207792No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT57090.2787325456498389RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC56780.27721902157992384No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG56600.27634020115223124RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA56230.274533736939752RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC55460.2707743384435114No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG54680.2669661165901767RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA54510.26613611951957816No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA54230.2647690655209452RNA PCR Primer, Index 1 (97% over 37bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC53340.2604237867395762RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG53220.25983790645444776RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCG52990.258714969241285RNA PCR Primer, Index 40 (100% over 51bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCT52040.25407675031735183RNA PCR Primer, Index 40 (100% over 51bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT49610.2422126745435016RNA PCR Primer, Index 1 (100% over 30bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC49570.2420173811151255RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC48590.23723269211991016No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG48340.23601210819255933RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC47620.23249682648178888RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG45520.2222439214920418RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT43480.2122839566448589RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT43460.2121863099306708RNA PCR Primer, Index 1 (100% over 25bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC42650.2082316180060541RNA PCR Primer, Index 1 (97% over 36bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA41310.20168928815545356RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG41230.20129870129870128RNA PCR Primer, Index 40 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT40960.19998047065716237RNA PCR Primer, Index 40 (100% over 41bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG40170.1961234254467337RNA PCR Primer, Index 40 (100% over 45bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC38730.18909286202519285RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA38680.18884874523972267No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG36890.18010936431989064RNA PCR Primer, Index 1 (100% over 29bp)
GCCGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC36720.17927936724929208RNA PCR Primer, Index 40 (100% over 42bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG36310.1772776096084367RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC36100.17625231910946199RNA PCR Primer, Index 1 (100% over 27bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAG35990.1757152621814276RNA PCR Primer, Index 40 (100% over 38bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC35780.1746899716824529RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC35720.17439703153988867RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA35280.17224880382775118RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA35200.17185821697099893No Hit
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC34640.16912410897373303RNA PCR Primer, Index 40 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTC34510.1684894053315106RNA PCR Primer, Index 40 (100% over 44bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC34330.16761058490381797RNA PCR Primer, Index 2 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33870.16536471047749243RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC33570.16390000976467142RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG33570.16390000976467142RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA33560.1638511864075774RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC33360.16287471926569672No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC33300.1625817791231325No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA32690.15960355434039644RNA PCR Primer, Index 40 (100% over 40bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC32580.1590664974123621RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG31530.15394004491748853RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG31440.15350063470364222RNA PCR Primer, Index 40 (100% over 45bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT30400.14842300556586271RNA PCR Primer, Index 1 (97% over 35bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC30280.1478371252807343RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA30250.1476906552094522RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA30120.14705595156722975RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC30030.14661654135338345No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG29830.1456400742115028RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA29560.14432184356996386No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG28930.14124597207303974Illumina Small RNA Adapter 2 (100% over 21bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT28920.14119714871594571RNA PCR Primer, Index 1 (100% over 30bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA28850.14085538521628746RNA PCR Primer, Index 1 (97% over 37bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG28610.13968362464603065RNA PCR Primer, Index 40 (100% over 45bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC28190.13763304364808124RNA PCR Primer, Index 1 (100% over 24bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC27690.13519187579337955RNA PCR Primer, Index 1 (100% over 24bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA27500.13426423200859292RNA PCR Primer, Index 1 (100% over 32bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC27420.13387364515184064RNA PCR Primer, Index 1 (97% over 36bp)
TTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTT27180.13270188458158383RNA PCR Primer, Index 40 (100% over 51bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC27130.13245776779611365RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT26340.128600722585685RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC26290.12835660580021482RNA PCR Primer, Index 2 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG26160.1277219021579924RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT26140.12762425544380432RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT26070.12728249194414606RNA PCR Primer, Index 1 (97% over 35bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA25590.12493897080363245RNA PCR Primer, Index 40 (100% over 40bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA24870.12142368909286202No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC24790.12103310223610976RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG24480.1195195781661947RNA PCR Primer, Index 1 (100% over 29bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCT24430.11927546138072453RNA PCR Primer, Index 40 (100% over 43bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC24250.11839664095303193RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG23980.11707841031149302No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG23550.11497900595644957No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT23450.11449077238550923RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG23370.11410018552875695No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT23230.11341665852944048RNA PCR Primer, Index 40 (100% over 41bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA23080.11268430817302998RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG22940.11200078117371351RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG22800.11131725417439704RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA22790.11126843081730299No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC22760.1111219607460209RNA PCR Primer, Index 2 (100% over 34bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22620.11043843374670442RNA PCR Primer, Index 1 (100% over 33bp)
CTCGGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC22180.10829020603456693RNA PCR Primer, Index 40 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGTG21790.10638609510789962No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC21370.1043355141099502RNA PCR Primer, Index 1 (97% over 36bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT20840.10174787618396641RNA PCR Primer, Index 1 (97% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA20750.10130846597012011RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG20650.10082023239917977No Hit

[FAIL]Adapter Content

Adapter graph