FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967990
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1779255.994797826138228RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC423421.4266220573519452RNA PCR Primer, Index 38 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA358071.2064393747957372RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC337221.136189811960283RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG334481.1269579749257914RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT293570.989120583290375RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA275900.9295853422686734RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT260520.8777657606663096RNA PCR Primer, Index 1 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG179960.6063362747179067RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC171880.579112463316925RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA137800.46428727859595215No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG132000.44474543377841563RNA PCR Primer, Index 3 (97% over 37bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC124090.41809440058760305RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC116680.3931280091914056RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC112800.38005518886519163RNA PCR Primer, Index 38 (100% over 38bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA104390.35171951387976375RNA PCR Primer, Index 38 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG102120.3440712401322107RNA PCR Primer, Index 1 (100% over 29bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA99610.33561433832324233RNA PCR Primer, Index 38 (100% over 40bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTC96440.3249337093453819RNA PCR Primer, Index 38 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG95090.3203851764999208RNA PCR Primer, Index 3 (97% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA88740.2989902257083077No Hit
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT87260.29400368599624666RNA PCR Primer, Index 1 (97% over 35bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC80550.2713957931125105No Hit
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT79470.26775696683614164RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA78230.2635790551854959RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA75130.2531342760588816RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG74430.25077577754642033No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT74030.24942806411072813RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC74020.24939437127483582RNA PCR Primer, Index 1 (100% over 31bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG73110.24632832320863615RNA PCR Primer, Index 3 (97% over 37bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC72930.24572185216257467RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT69890.2354792300513142RNA PCR Primer, Index 38 (100% over 39bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC69770.23507491602060654RNA PCR Primer, Index 2 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG68040.22924605541123788RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG67770.2283363488421457RNA PCR Primer, Index 3 (97% over 37bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC65990.22233902405331554RNA PCR Primer, Index 2 (100% over 34bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC65500.22068807509459265RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC63360.21347780821363954RNA PCR Primer, Index 1 (100% over 31bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG63050.21243333030097808RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC62700.21125408104474747No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA61050.20569476312251728No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC60080.20242655804096374RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59710.20117992311294847RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA59530.20057345206688704RNA PCR Primer, Index 1 (100% over 32bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC58650.19760848250836424RNA PCR Primer, Index 38 (100% over 38bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC58530.1972041684776566RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA57660.19427289175502613No Hit
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT57540.19386857772431848RNA PCR Primer, Index 38 (100% over 39bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC56660.1909036081657957RNA PCR Primer, Index 2 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT56650.1908699153299034RNA PCR Primer, Index 1 (97% over 35bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT55610.18736586039710376RNA PCR Primer, Index 1 (100% over 30bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA54550.1837944197925195RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC51910.1748995111169512RNA PCR Primer, Index 1 (100% over 24bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC50710.1708563708098747RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC50680.1707552923021978RNA PCR Primer, Index 2 (100% over 34bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50140.16893587916401337RNA PCR Primer, Index 1 (100% over 31bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT49920.16819463677438265RNA PCR Primer, Index 1 (97% over 35bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA49890.16809355826670574RNA PCR Primer, Index 2 (97% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA49120.16549920990299832RNA PCR Primer, Index 2 (97% over 36bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA48080.1619951549701987RNA PCR Primer, Index 38 (100% over 40bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA47860.161253912580568RNA PCR Primer, Index 1 (100% over 32bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC45480.15323501763819958RNA PCR Primer, Index 1 (100% over 33bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCG44290.14922557016701538RNA PCR Primer, Index 38 (100% over 51bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG44040.14838324926970778RNA PCR Primer, Index 1 (100% over 29bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC42870.14444118747030818RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC41380.13942095492235484RNA PCR Primer, Index 1 (100% over 33bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC41280.1390840265634318RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG40180.13537781461527837Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC39820.1341648725231554RNA PCR Primer, Index 2 (100% over 34bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA38980.13133467430820184RNA PCR Primer, Index 38 (100% over 40bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA38230.12880771161627902RNA PCR Primer, Index 1 (100% over 22bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC37900.12769584803183298RNA PCR Primer, Index 38 (100% over 38bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA37720.1270893769857715RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG37000.12466349280152561RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA36670.12355162921707957RNA PCR Primer, Index 1 (100% over 32bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG36640.12345055070940265No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG36170.12186698742246435No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG35990.1212605163764029RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA35690.12024973129963377RNA PCR Primer, Index 1 (100% over 28bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC35170.11849770383323394RNA PCR Primer, Index 38 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA33710.11357854979295752No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC33240.11199498650601922RNA PCR Primer, Index 1 (100% over 27bp)
CTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCT33100.11152328680352697RNA PCR Primer, Index 38 (100% over 51bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC31660.10667151843503515RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA31640.10660413276325055RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC31620.10653674709146595No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA31300.1054585763429122No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT31200.10512164798398915RNA PCR Primer, Index 1 (100% over 25bp)
TCGTACAACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC31000.10444779126614308RNA PCR Primer, Index 1 (100% over 33bp)
TACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT30960.10431301992257387RNA PCR Primer, Index 38 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC30430.10252729962028173No Hit
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC30390.10239252827671252RNA PCR Primer, Index 2 (100% over 34bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTAT29920.10080896498977422RNA PCR Primer, Index 38 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG207.0205727E-445.018881
GGGGGTA700.045.018881
GCATCGT253.8826744E-545.018881
AACGGAA1050.045.012812
CTACGAC406.8048394E-945.012812
ACGAGGT253.8857674E-545.012812
GTAAGGT551.8189894E-1245.0128062
GCACGAC351.2107193E-745.0097773
TAAAGTA207.027571E-445.0097735
CATTGCC551.8189894E-1245.0097735
CATTCAT207.027571E-445.0097733
AATGCTA453.8380676E-1045.0097733
GATTAGC207.027571E-445.0097735
ACGACTA207.027571E-445.0097735
AGAGACC207.027571E-445.0097734
GTCGACC207.035159E-444.99991621
ATTCTAC351.2133023E-744.9976467
TGTGACA302.1674496E-644.9976438
CACTACA2350.044.9976437
CGGTTAT253.895058E-544.9946116
Produced by FastQC (version 0.11.5)