FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381841
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC792643.327845981322851RNA PCR Primer, Index 7 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG456331.915870958640816RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC448031.8810239642360678RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG344941.4482074999968513RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA340081.4278031153212998RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG271911.1415959335656747RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC267871.12463426399999RNA PCR Primer, Index 1 (100% over 31bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC263811.1075886257730891RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA247651.0397419475103502No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG233440.9800822137161969RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC173440.7281762300674143RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA165720.6957643268379375RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG137590.5776624048372666RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA131300.5512542608847526RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG128990.5415558805142745RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG126010.5290445499930516RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC105240.4418430953199647RNA PCR Primer, Index 7 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA103650.43516758675327194No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC102500.4303393887333369RNA PCR Primer, Index 7 (100% over 42bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT94730.39771756385081963RNA PCR Primer, Index 7 (100% over 41bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC90850.38142764357486497No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA88070.36975599966580475RNA PCR Primer, Index 7 (100% over 35bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG84190.3534660793898501RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA83280.3496455053045103RNA PCR Primer, Index 1 (100% over 28bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT80500.33797386139545RNA PCR Primer, Index 1 (100% over 30bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA77250.3243289539478076RNA PCR Primer, Index 7 (100% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC76970.32315339269078RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA76430.32088623883794093No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC75130.31542827585888394RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG74370.31223746673266606RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT71890.3018253527418497RNA PCR Primer, Index 7 (100% over 41bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT71380.2996841518808351RNA PCR Primer, Index 1 (100% over 30bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA68950.28948195954305933RNA PCR Primer, Index 7 (100% over 40bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG68290.28671099372292275RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA66130.2776423783115666RNA PCR Primer, Index 7 (100% over 35bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC64130.2692455121899405RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG59190.24850525286952405RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT58870.24716175429006387RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC57000.23931068446634346RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG56130.23565804770343612RNA PCR Primer, Index 1 (100% over 29bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG56110.23557407904221983No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC55710.23389470581789465RNA PCR Primer, Index 7 (100% over 39bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT55290.23213136393235317RNA PCR Primer, Index 7 (100% over 46bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC55180.23166953629566375RNA PCR Primer, Index 2 (100% over 34bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC54240.22772300921849947RNA PCR Primer, Index 1 (100% over 27bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC53910.22633752630843115RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC52510.22045972002329292RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA51810.21752081688072378RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT50610.21248269720774812RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT50360.21143308894254484RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG50280.21109721429767983RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT49600.20824227981632695RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA48880.20521940801254157RNA PCR Primer, Index 1 (100% over 32bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT47850.20089502195990414RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC46060.19337982678104879RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC45900.19270807749131869No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT45250.18997909600179022RNA PCR Primer, Index 7 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG44330.18611653758584223No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA43760.18372343074117878No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA43300.18179215153320477RNA PCR Primer, Index 7 (100% over 35bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG43130.18107841791286655RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA43040.18070055893739337RNA PCR Primer, Index 7 (100% over 37bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG42500.17843340508455433RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG40870.17158995919542908Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC40680.17079225691387462No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG40360.16944875833441442RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC39870.16739152613461605No Hit
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC38950.16352896771866804RNA PCR Primer, Index 7 (100% over 39bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA38120.16004426827819324RNA PCR Primer, Index 1 (100% over 32bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG37560.15769314576413793RNA PCR Primer, Index 7 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG37100.1557618665561639Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT37080.15567789789494763RNA PCR Primer, Index 1 (100% over 30bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA36580.15357868136454111RNA PCR Primer, Index 7 (100% over 40bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT35990.15110160585866142RNA PCR Primer, Index 1 (100% over 30bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG35910.15076573121379638RNA PCR Primer, Index 7 (100% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA35360.14845659303034922No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA35320.1482886557079167RNA PCR Primer, Index 7 (100% over 35bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG34180.14350244201858983Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG34090.14312458304311665Illumina Small RNA Adapter 2 (100% over 21bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC34080.14308259871250853RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG33300.13980782092507432RNA PCR Primer, Index 7 (100% over 36bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT33260.13963988360264185RNA PCR Primer, Index 7 (100% over 38bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA32730.13741471408041092RNA PCR Primer, Index 7 (100% over 35bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC32720.1373727297498028RNA PCR Primer, Index 2 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC32410.13607121550095075RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC31920.13401398330115236RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC31780.13342620267263852No Hit
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT31440.13199873543196208RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA30930.12985753457094742RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC30690.12884991063635232RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG30500.12805220835479783Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG30420.1277163337099328RNA PCR Primer, Index 7 (100% over 45bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC29350.12322401033486281RNA PCR Primer, Index 1 (100% over 27bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG29210.122636229706349RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC29070.12204844907783517RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA29030.12188051175540265RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA29030.12188051175540265RNA PCR Primer, Index 7 (100% over 37bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG28490.11961335790256361RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG28270.11868970262918473RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA28110.11801795333945465RNA PCR Primer, Index 7 (100% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC27230.11432333224593917RNA PCR Primer, Index 1 (100% over 26bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG26720.11218213138492453RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA26690.11205617839310013RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC26440.11100657012789686RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT26290.1103768051687749RNA PCR Primer, Index 7 (100% over 43bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC26070.10945314989539603RNA PCR Primer, Index 7 (100% over 39bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC25720.10798369832411146RNA PCR Primer, Index 1 (100% over 33bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC25340.10638829376100252RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT25320.10630432509978625RNA PCR Primer, Index 1 (100% over 25bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT25130.10550662281823178RNA PCR Primer, Index 7 (100% over 41bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC24680.1036173279408659RNA PCR Primer, Index 1 (100% over 24bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG24470.10273565699809517RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC24320.10210589203897322RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG24280.10193795471654069RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGGG502.1827873E-1145.006621
GCAGCGA453.8380676E-1045.006621
AGCTACA5700.045.006621
TACGGGG207.02972E-445.0066151
AGCTATA207.02972E-445.0066151
TCAGTGC7550.045.0066151
ACGGGTC700.045.006611
TTATAAT302.1642954E-645.006611
TCCCACA1100.045.006611
GTACGCC600.045.006611
CCTTAAT406.8139343E-945.0047262
AGCGGGA207.0311746E-445.0047262
TATAATA800.045.0047262
GTAACAG207.0311746E-445.0047262
GAGATAA253.8896567E-545.0047262
GCTACAT5700.045.0047232
TTTTCGC253.8906215E-545.0028424
AATGCGC302.165547E-645.00283422
ATGCGCC302.165547E-645.00283423
CAGCACA1000.045.0018924
Produced by FastQC (version 0.11.5)