FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390902
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC153643.9303968769666056RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC115642.95828622007562RNA PCR Primer, Index 47 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA68821.7605435633483584RNA PCR Primer, Index 47 (100% over 37bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG64101.6397971870187413RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC45441.1624396907664836RNA PCR Primer, Index 5 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC38450.9836224936173261RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC35420.9061094596599659RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC30990.7927818225539905No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC29840.7633626842533423RNA PCR Primer, Index 1 (100% over 27bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTC27430.7017104031189403RNA PCR Primer, Index 47 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA27100.6932683895196239RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG25810.660267790904114RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG23750.6075691605568658RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG22930.5865920358555341RNA PCR Primer, Index 10 (97% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA22770.5824989383528353RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA21970.5620334508393409RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC21380.5469401537981387RNA PCR Primer, Index 5 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG21380.5469401537981387RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG21330.5456610608285453RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG21050.5384981401988221RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA20610.5272421220664003No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG20600.5269863034724815RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT19010.4863111470394114RNA PCR Primer, Index 3 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT16500.4221006799658226RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA16050.41058884323948197RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA16040.4103330246455633No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG14730.3768207888422162RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA14410.36863459383681846RNA PCR Primer, Index 47 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG14220.3637740405523635RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA14190.3630065847706075RNA PCR Primer, Index 1 (100% over 28bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG13240.3387038183483328RNA PCR Primer, Index 47 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA12470.31900578661659446No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11490.29393556441256374RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11210.2867726437828407RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG11210.2867726437828407RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG10670.27295843971123196RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG10220.26144660298489136RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG10160.25991169142137927RNA PCR Primer, Index 10 (97% over 36bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCG10140.2594000542335419RNA PCR Primer, Index 47 (100% over 51bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG9970.25505113813692437RNA PCR Primer, Index 47 (100% over 45bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT9130.23356237624775517RNA PCR Primer, Index 3 (100% over 34bp)
CTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCT9100.23279492046599914RNA PCR Primer, Index 47 (100% over 51bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG9020.2307483717146497RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA8630.22077144655182115RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA8340.21335270732817943RNA PCR Primer, Index 47 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT8240.21079452138899266RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGT7960.2036316007592696RNA PCR Primer, Index 47 (100% over 46bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT7830.20030595903832676RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG7810.19979432185048937RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA7790.19928268466265203No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC7750.1982594102869773No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA7710.1972361359113026No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG7580.19391049419035972RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT7550.1931430384086037RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC7480.19135230825117294No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC7280.1862359363727993No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA7100.18163120168226307No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG7070.18086374590050705RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC6790.17370082527078398No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA6680.1708868207376785RNA PCR Primer, Index 47 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC6380.1632122629201181No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT6340.16218898854444336RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC6310.16142153276268734No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGAT6300.16116571416876863RNA PCR Primer, Index 47 (100% over 41bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA6240.15963080260525656RNA PCR Primer, Index 1 (100% over 22bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG6160.15758425385390712RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC6110.1563051608843137RNA PCR Primer, Index 47 (100% over 44bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCT6020.15400279353904558RNA PCR Primer, Index 47 (100% over 51bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC5610.1435142311883797RNA PCR Primer, Index 47 (100% over 42bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCT5480.14018858946743684RNA PCR Primer, Index 47 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC5440.13916531509176214No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA5420.13865367790392477RNA PCR Primer, Index 47 (100% over 37bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT5150.13174657586812039RNA PCR Primer, Index 1 (100% over 30bp)
TCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTC5030.12867675274109625RNA PCR Primer, Index 47 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA5030.12867675274109625No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA5020.12842093414717753RNA PCR Primer, Index 47 (100% over 38bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC4880.12483947383231603RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC4850.12407201805055999RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG4840.12381619945664131RNA PCR Primer, Index 10 (97% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG4770.12202546929921054No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA4740.1212580135174545RNA PCR Primer, Index 1 (100% over 28bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC4610.11793237179651166RNA PCR Primer, Index 47 (100% over 42bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC4590.1174207346086743RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA4400.11256018132421938No Hit
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG4400.11256018132421938RNA PCR Primer, Index 47 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT4360.11153690694854464RNA PCR Primer, Index 3 (100% over 34bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4340.1110252697607073RNA PCR Primer, Index 1 (100% over 32bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT4340.1110252697607073RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4320.11051363257286993RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4270.10923453960327652RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4210.10769962803976443RNA PCR Primer, Index 1 (100% over 30bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG4040.10335071194314688RNA PCR Primer, Index 47 (100% over 45bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA3960.10130416319179743RNA PCR Primer, Index 1 (100% over 23bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3950.10104834459787874RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTCA11200.045.042511
AGCTCGG253.8644517E-545.042511
TGCCGTG206.9949473E-445.042511
AACCCGT406.7393557E-945.042511
TAGTTTA206.9949473E-445.042511
GGGGGTA351.2002238E-745.042511
TACCCTG4400.045.042511
TTAATGC206.9949473E-445.042511
GTAGTCG650.045.042511
TGTAAAC1950.045.042511
CATGCCT206.9949473E-445.042511
GTCTACG2250.045.0425071
GTGAAAT600.045.0425071
CGTGACC302.1478027E-645.0425071
CAACGGA1500.045.0425071
GTAGCGT351.206281E-745.0136952
TTTCCGT3950.045.013692
CCTTGGT207.017041E-445.013692
TAATGCT207.017041E-445.013692
CGGATCC207.017041E-445.013692
Produced by FastQC (version 0.11.5)