FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749086
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA19298611.033534085802527No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA650083.7166840281152558No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC556963.1842916814839293No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC549923.1440420882678155No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA481192.751093999951975No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA426342.437501643715632No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC374522.1412326209231565No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA371092.121622378773828No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA361012.0639922793962104No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC341931.9549067341457196No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC218851.2512249254753627RNA PCR Primer, Index 1 (100% over 51bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC207791.1879918997693653No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA164820.9423207320852148No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC163190.9330015791104611RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG162240.9275701709349912No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA143140.8183702802492273No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC142490.8146540536028531RNA PCR Primer, Index 1 (100% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC140930.8057351096515551No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG137440.7857818311964077RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA123570.7064832718345467No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA111720.6387336014352639No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG108980.6230682768028559RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG105170.6012854713833397RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA94590.5407967361238956No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA94510.5403393543828033No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG87730.501576251825239RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC85300.4876832814395633RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA82510.47173209321897264No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA76300.4362278355666903RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG75430.43125380913231254No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG73070.4177610477700925RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA71050.4062121588075143No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT66790.38185658109435444RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG61140.34955399562971745RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA60940.34841054127698695No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA58750.33588971611458784RNA PCR Primer, Index 1 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT51060.2919238962520997RNA PCR Primer, Index 1 (100% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG49450.282719088712619RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC48520.27740202597242214RNA PCR Primer, Index 1 (100% over 31bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA45940.26265146482219853RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA44460.25418990261199276No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG43390.24807242182488454No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGAATTCTCGGGTGCCA41380.2365807055799429No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG37950.2169704634306146Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG36190.2069080651265861RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA35910.20530722903276338No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA35310.20187686597457188No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC33410.191014049623632No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC33180.18969907711799192RNA PCR Primer, Index 1 (100% over 33bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT32200.1840961507896124RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG32080.18341007817797408RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC31830.18198076023706097No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGCC31120.17792149728486764No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA30290.173176161721036No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA30080.17197553465066898No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA28920.165343499404832RNA PCR Primer, Index 1 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA27830.15911167318245073No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA27640.15802539154735673RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT27410.15671041904171665RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTTGGAATTCTCGGGTGCC27340.15631021001826095No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTTGGAATTCTCGGGTGCCA27200.1555097919713496No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT26430.15110749271333715RNA PCR Primer, Index 1 (100% over 41bp)
CACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCACCATGGAATTCTCG25680.1468195388905977No Hit
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG25370.14504718464386543RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA24440.13973012190366854RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG24090.13772907678639015RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA23660.13527064992801954No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC23560.1346989227516543RNA PCR Primer, Index 1 (100% over 38bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG23300.13321243209310463RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT23150.13235484132855674RNA PCR Primer, Index 1 (100% over 46bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG21320.12189223400107256RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT21190.12114898867179773RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA21010.12011987975434027RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG20510.11726124387251399No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT20510.11726124387251399RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG19880.11365936266141288Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCA19850.1134878445085033No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA19820.11331632635559373RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC19580.1119441811323171RNA PCR Primer, Index 1 (100% over 27bp)
TGAAATGTTTAGGACCACTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG19540.11171549026177102RNA PCR Primer, Index 1 (100% over 29bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT18720.10702732741557591RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG17940.1025678554399269RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC17810.10182461011065207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGGA453.8380676E-1045.0196531
TCCTTCA25650.045.0196531
TGTCAGT253.8816233E-545.019651
TTAATGC253.8816233E-545.019651
GTCTACG3900.045.019651
GACCCAG1200.045.019651
GCATTGA253.8816233E-545.019651
GCTTCTA253.8816233E-545.019651
AGGCAAG1700.045.019651
TCCCATA700.045.019651
GCCCGAC2050.045.019651
GCATCGG302.1596534E-645.019651
AAGCTGC8250.045.019651
TGTGCAA406.7921064E-945.0196461
GTTTTCG207.019186E-445.0196461
CACCGAC207.019186E-445.0196461
CCTTCAT25650.045.0093542
GCTGGTC3550.045.0093542
CATAGGT900.045.0093542
CGAGGAG453.8380676E-1045.0093542
Produced by FastQC (version 0.11.5)