FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10508024
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC3082902.9338532154094814RNA PCR Primer, Index 25 (100% over 51bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA992920.9449159994305303RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG981560.9341052133112753RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT925370.8806317914766848RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA898550.8551084390366828RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA872630.8304415749335936RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC872020.8298610661719082RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA868060.8260925174894919RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA842850.802101327518856RNA PCR Primer, Index 25 (100% over 38bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG763040.7261498451088425RNA PCR Primer, Index 5 (97% over 37bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC755260.718745979263085RNA PCR Primer, Index 25 (100% over 42bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG754920.7184224170024736RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA714690.6801373883424704No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG615940.5861615847089805RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA596720.5678708004473534RNA PCR Primer, Index 25 (100% over 40bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA596540.5676995027799708RNA PCR Primer, Index 1 (100% over 32bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT549520.5229527454448144RNA PCR Primer, Index 25 (100% over 41bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG545040.5186893368344039RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG542640.5164053679359697RNA PCR Primer, Index 5 (97% over 37bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG532710.5069554466186983RNA PCR Primer, Index 5 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC498180.4740948440924764RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA463170.44077744778656763RNA PCR Primer, Index 1 (100% over 22bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT439820.4185563337122184RNA PCR Primer, Index 8 (100% over 36bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT426890.4062514512719042RNA PCR Primer, Index 8 (100% over 36bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT417100.39693476147370804RNA PCR Primer, Index 25 (100% over 39bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA414120.394098833424819RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA402440.3829835181191059RNA PCR Primer, Index 1 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC389440.3706120199192541RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC388030.369270188191424RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT374960.3568320742320345RNA PCR Primer, Index 1 (100% over 30bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA374240.35614688356250424RNA PCR Primer, Index 25 (100% over 40bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG371990.35400566272022216RNA PCR Primer, Index 1 (100% over 29bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA368750.35092230470733604RNA PCR Primer, Index 25 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA359130.34176739603944567No Hit
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA353200.33612408955289785RNA PCR Primer, Index 25 (100% over 38bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC349760.3328504007984755RNA PCR Primer, Index 5 (100% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC339540.32312449990597664RNA PCR Primer, Index 5 (100% over 35bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG333740.3176049084014273RNA PCR Primer, Index 5 (97% over 37bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC333250.31713859808466366No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC332660.3165771223971319RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC327870.31201870113734037RNA PCR Primer, Index 25 (100% over 44bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC325950.310191526018593RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA325850.310096360647825No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC310370.29536476125292443RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT303350.28868415222500443RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG289500.27550374837362385RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC285470.2716685839316697RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC276980.26358904395345883RNA PCR Primer, Index 5 (100% over 35bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA276180.26282772098731405RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC274990.2616952530751738RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC274310.26104812855395076RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG271080.2579742870781414RNA PCR Primer, Index 25 (100% over 45bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT267720.25477673062033357RNA PCR Primer, Index 25 (100% over 39bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC265840.25298762164989347RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG264000.25123657882776057No Hit
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT262630.24993281324823774RNA PCR Primer, Index 25 (100% over 41bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA250370.23826553879206977RNA PCR Primer, Index 25 (100% over 38bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC248310.23630513215424706RNA PCR Primer, Index 5 (100% over 35bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC246920.2349823335005706RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG236300.22487577112499935RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA236090.22467592384638632RNA PCR Primer, Index 1 (100% over 34bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT233310.2220303265390334RNA PCR Primer, Index 25 (100% over 39bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA231630.22043154831012948RNA PCR Primer, Index 1 (100% over 32bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA228140.2171102768703231RNA PCR Primer, Index 1 (100% over 23bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA223890.2130657486126792RNA PCR Primer, Index 1 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC223000.2122187768128432RNA PCR Primer, Index 1 (100% over 33bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA214490.20412020376047868RNA PCR Primer, Index 25 (100% over 40bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG209380.19925725331422922Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA206980.19697328441579504RNA PCR Primer, Index 25 (100% over 40bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT203560.1937186287355263RNA PCR Primer, Index 1 (100% over 30bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC201400.19166305672693554RNA PCR Primer, Index 1 (100% over 33bp)
AAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT197270.1877327269142134RNA PCR Primer, Index 25 (100% over 43bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT196850.1873330323569874RNA PCR Primer, Index 25 (100% over 39bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG195700.18623863059315432RNA PCR Primer, Index 1 (100% over 29bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA195660.18620056444484712No Hit
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA194900.1854773076270096RNA PCR Primer, Index 1 (100% over 34bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA193500.18414499243625632RNA PCR Primer, Index 1 (100% over 34bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG189800.18062387371783695No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT189610.18044305951337758RNA PCR Primer, Index 25 (100% over 39bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA188020.17892993011816494RNA PCR Primer, Index 25 (100% over 38bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT185070.17612255168050625RNA PCR Primer, Index 25 (100% over 46bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA180770.17203044073747834RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT173990.16557822859940174RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG172550.16420784726034124RNA PCR Primer, Index 1 (100% over 29bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT160210.1524644405075588RNA PCR Primer, Index 8 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT159700.15197909711664154RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC156480.148914772177909RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA156060.148515077620683No Hit
GTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT151530.1442040863248885RNA PCR Primer, Index 25 (100% over 39bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT149270.14205334894552962RNA PCR Primer, Index 25 (100% over 41bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG148710.1415204228692283Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT146890.13978841312124907RNA PCR Primer, Index 25 (100% over 39bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG144810.13780897340927276RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC144280.13730459694420188RNA PCR Primer, Index 5 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC142630.13573436832652838RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA142380.13549645489960815No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT140600.13380251129993612RNA PCR Primer, Index 1 (100% over 25bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC139390.13265101031364224RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC139150.1324226134237988RNA PCR Primer, Index 1 (100% over 33bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA137280.1306430209904355RNA PCR Primer, Index 25 (100% over 38bp)
ATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGA135160.12862551513015197RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC131030.12469518531742979No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT130100.12381014736928657RNA PCR Primer, Index 25 (100% over 41bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA129640.12337238666375333RNA PCR Primer, Index 25 (100% over 38bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT128750.12252541486391733RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC127820.12164037691577408RNA PCR Primer, Index 1 (100% over 26bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA127380.12122164928439448No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA127140.12099325239455105RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC125870.11978465218579631No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT123580.117605365195207RNA PCR Primer, Index 1 (100% over 25bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC117270.11160043029974046RNA PCR Primer, Index 5 (100% over 35bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG116760.11111508690882319No Hit
CTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC114190.10866933688008326RNA PCR Primer, Index 25 (100% over 42bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA113570.10807931158132109RNA PCR Primer, Index 1 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC113460.1079746296734762RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG110410.10507208586504943RNA PCR Primer, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG406.7975634E-945.020111
CATCCGT207.0204417E-445.020111
ATAGCGG453.8380676E-1045.009193
AAATCGA253.888294E-545.0091823
AAATCTA406.8230293E-945.000836
AGTAGCG207.035271E-445.000836
GTACAAT302.1677733E-644.99826418
CATACGT207.0374156E-444.9980514
TGAGGTA62000.044.94751
TCCTTCA150350.044.8703961
GTCTACG184850.044.8252751
AGCTTAT236700.044.7837752
TTCACAG45150.044.671121
CCTTCAT151150.044.6695062
TCAAGTA16800.044.610082
TAGCTTA237900.044.584861
GAGGTAG62650.044.580892
GCTTATC234950.044.578153
CTTCATT155050.044.530213
CACTACA7700.044.413667
Produced by FastQC (version 0.11.5)