FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5024519
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC4410388.777715837078135RNA PCR Primer, Index 4 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1729033.4411851164260696RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1196662.381640909308931RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC640711.2751668368653797RNA PCR Primer, Index 4 (100% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG611371.216773187642439RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC372590.7415436184040701RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA316870.6306474311272382No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA291910.5809710342422827RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA287280.5717562218393442RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT279910.5570881511245156RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA234120.46595504962763595RNA PCR Primer, Index 2 (97% over 36bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC225110.44802298488671255RNA PCR Primer, Index 1 (100% over 26bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA218180.43423061988620204RNA PCR Primer, Index 4 (100% over 39bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC212800.42352312728840313RNA PCR Primer, Index 1 (100% over 26bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC205130.40825798449563033RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT199670.39739127267704627RNA PCR Primer, Index 3 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA195450.38899245878063154No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA194540.38718134014420086RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG193520.38515129507919066RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA191730.38158876501412375No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG188450.3750607769619341No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC181240.3607111446886757RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG176410.3510982842337744RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC170200.33873889222032993RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC167080.3325293426097105No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG160630.3196922929339107RNA PCR Primer, Index 2 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG160480.3193937568949386RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC160360.31915492806376095RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT156130.310736211764748RNA PCR Primer, Index 1 (100% over 30bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA151800.30211847143975373RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA147760.2940779007901055No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG143580.2857586965040833RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG139420.27747929702325735RNA PCR Primer, Index 2 (97% over 35bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG135700.27007560325674956Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT133620.26593590351633656RNA PCR Primer, Index 3 (100% over 34bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC132740.26418449208770034RNA PCR Primer, Index 4 (100% over 37bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT132080.26287093351622315RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA131000.2607214740356241RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA130060.258850648191399RNA PCR Primer, Index 1 (100% over 32bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC127830.25441241241201396RNA PCR Primer, Index 1 (100% over 33bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG126690.2521435385158261Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA126450.25166588085347075No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA126280.2513275400093024RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC122440.24368501741161688RNA PCR Primer, Index 4 (100% over 37bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA122220.24324716455445786RNA PCR Primer, Index 2 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC117620.2340920593593138RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG114890.2286587034500218Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG114500.22788250974869434RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA113800.2264893415668246RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC113640.22617090312525437RNA PCR Primer, Index 1 (100% over 24bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT113320.22553402624211394RNA PCR Primer, Index 3 (100% over 34bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA113190.22527529500833812RNA PCR Primer, Index 4 (100% over 40bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC110120.21916525741070936RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG101280.2015715335139543RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA101080.2011734854619915No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG100590.20019826773468263RNA PCR Primer, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC98150.19534208150073668No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC97240.19353096286430602RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA94500.18807770455241585RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC94370.18781897331864003RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCA94090.18726170604589215RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC91710.18252493422753502RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA89910.17894250175986995RNA PCR Primer, Index 2 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC89170.17746972396760766No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT88600.1763352870195137RNA PCR Primer, Index 1 (100% over 25bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC87580.1743052419545035RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT85510.1701854446166887RNA PCR Primer, Index 4 (100% over 41bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG85200.16956847013614637RNA PCR Primer, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC83790.16676223136980875No Hit
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG79410.15804497903182374Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG77550.15434313214856984Illumina Small RNA Adapter 2 (100% over 21bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG76480.15221357507056893RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG75690.15064128526531595RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT74910.14908889786266108RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG73280.1458448062391644No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC73150.1455860750053886RNA PCR Primer, Index 4 (100% over 38bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG69220.13776443078431985RNA PCR Primer, Index 4 (100% over 49bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT68560.13645087221284266RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT68520.1363712626024501RNA PCR Primer, Index 3 (100% over 34bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC67240.13382375506988828RNA PCR Primer, Index 4 (100% over 38bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC66270.13189322201786877RNA PCR Primer, Index 4 (100% over 37bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG65010.12938551929050324RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA64760.12888795922554974RNA PCR Primer, Index 1 (100% over 32bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC63670.12671859734235258RNA PCR Primer, Index 4 (100% over 37bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG63120.12562396519945493RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCCG62510.12440991864096841RNA PCR Primer, Index 4 (100% over 51bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA61990.12337499370586517RNA PCR Primer, Index 2 (97% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG60860.12112602221227545RNA PCR Primer, Index 1 (100% over 29bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA60350.12011099967977035RNA PCR Primer, Index 2 (97% over 36bp)
TGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT58770.11696642006926435RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG58260.11595139753675925Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC58150.11573247110817972RNA PCR Primer, Index 4 (100% over 44bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG57830.11509559422503925No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC57340.11412037649773044RNA PCR Primer, Index 1 (100% over 33bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT57320.11408057169253415RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA56160.11177189299115RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG55750.1109558944846263RNA PCR Primer, Index 1 (100% over 29bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC55630.11071706565344863RNA PCR Primer, Index 1 (100% over 33bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG55530.11051804162746721RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT54540.10854770377025144RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG53330.1061395130558766RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT53330.1061395130558766RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG53010.10550263617273613RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG52360.10420898000385709Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG51910.10331337188694081RNA PCR Primer, Index 2 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC50900.10130322922452875RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTGC10000.045.0289421
TCTTACG253.877941E-545.028941
TTATAAT253.877941E-545.028941
TTATAAA502.1827873E-1145.028941
TCGTACA207.013314E-445.028941
TCTTTTA253.877941E-545.028941
CACAAAT253.877941E-545.028941
AGTAAGG2750.045.028941
TCTACGT253.8843304E-545.0163882
TTGCGGA207.022953E-445.0163882
ACCCGTA24250.045.0163882
ATCGGTA253.8843304E-545.0163882
TATAATA302.1615451E-645.0163882
CGTAAGA351.2095552E-745.0163842
ACACTAG207.0270867E-445.0110133
CCCTATA253.8870716E-545.011013
CTACATT1800.045.011013
CCGGTTA950.045.011013
AGGGGCA302.163324E-645.011013
GTAAGAC502.1827873E-1145.011013
Produced by FastQC (version 0.11.5)