FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059145
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC2387035.880622643438458No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1560503.844405656856308RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA664841.6378818692113735No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA651841.6058554202917117No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG598461.4743498938815933RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC509551.2553136190010459No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC466071.1481974652297466RNA PCR Primer, Index 15 (100% over 51bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT465021.1456107135862355RNA PCR Primer, Index 1 (100% over 35bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG447381.1021532859752485RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG381100.9388676679448504No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA346850.8544902929065111RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA345140.8502775830870786No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC280090.6900221598390794RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC248700.6126906035630656No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA237000.5838667995353701No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT228820.5637147724459215RNA PCR Primer, Index 15 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA213380.5256772054213387No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA209960.5172517857824739No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT197100.4855702370819471RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG186940.46054033546473455RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG181080.4461037977209486No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC180490.44465028965459474RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA178260.43915652187837584RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC177930.4383435427904152RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA175410.4321353388459885No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC164990.4064649082503828No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT159390.39266889948499006RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA157300.38752003192790596No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG150400.37052137827054715RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG150210.3700532994017213RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA140180.3453436622737054RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC139430.34349598252834035No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG138450.3410816809943966RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA135420.33361705482312165No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA134500.3313505676688071RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC133200.32814792277684096RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC128260.3159778721873695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC124400.3064684804312238No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC124210.306000401562398RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA122530.30186159893278014No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA114170.2812661287044439No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG112100.2761665326072363Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGTG110160.2713872009992252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC107530.26490800402547826No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG102120.25158007412891137RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGAATTCTCGGGTGCCA100670.24800789328787218No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA96260.23714353638512545No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG95770.23593638561815355RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA93480.2302948034623055RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC93440.23019626054255268RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG93090.22933400999471565RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA90310.2224852770718957No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG88370.21770594546388464No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT87990.21676978772623298RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG87320.2151191938203735RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG87030.21440475765216568No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC83780.20639814542225024No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA83190.20494463735589635RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG81600.20102755629572236RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG81500.20078119899634037No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC80690.1987857048713461RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG79950.19696266085591918RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC78640.1937353802340148RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA76680.18890677716612733RNA PCR Primer, Index 1 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT75270.18543313924484098RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC71730.1767120908467177No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG71230.17548030434980766RNA PCR Primer, Index 15 (100% over 36bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG69390.17094733004117862Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT69310.170750244201673RNA PCR Primer, Index 1 (100% over 35bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA69140.17033143679272358RNA PCR Primer, Index 1 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC67170.16547819799489794RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA65750.16197992434367336No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC65650.16173356704429134RNA PCR Primer, Index 1 (100% over 33bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA63810.1572005927356623No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT63640.15678178532671289RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC61470.15143583193012322RNA PCR Primer, Index 1 (100% over 27bp)
GTCCGGATGATCCTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA60190.14828245849803345RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG60120.14811000838846605Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59870.147494115140011RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA58330.14370021272952802RNA PCR Primer, Index 15 (100% over 40bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA57990.14286259791162917RNA PCR Primer, Index 1 (100% over 28bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG57310.14118736827583148No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA55540.13682684407676984No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG55170.13591532206905643Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG54730.13483134995177556RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA54330.1338459207542475RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA53830.13261413425733745RNA PCR Primer, Index 1 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT52880.13027373991320834RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT52860.13022446845333194RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT50780.12510023662618608RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA50420.1242133503484108RNA PCR Primer, Index 1 (100% over 34bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG50280.123868450129276RNA PCR Primer, Index 15 (100% over 45bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA49250.12133096994564126RNA PCR Primer, Index 1 (100% over 22bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG48930.12054262658761881Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT48820.1202716335582986RNA PCR Primer, Index 1 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG48450.11936011155058517Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT48380.11918766144101776RNA PCR Primer, Index 1 (100% over 25bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT48260.11889203268175934RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG47690.11748779607528186No Hit
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG46580.11475323005214152RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC46010.11334899344566404No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA45210.11137813505060795RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA44460.10953045530524286RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG43890.10812621869876538RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG42400.10445549493797339RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC41850.10310052979137233RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA41680.1026817223824229RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA41420.10204119340402966No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC41030.10108039993643982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCG206.8704324E-445.2172621
ACGGGTC253.783402E-545.2172621
CATTAGG406.537448E-945.2172621
GCATTCG551.8189894E-1245.2172621
CACAAAT206.8704324E-445.2172621
CAACGGA42300.045.1103631
TGAGGTA50050.045.0817451
GTCTACG91600.045.069171
GGCGAAT351.2135388E-744.99724618
CTAGTAT302.1677806E-644.99724217
ATAAGCG253.893968E-544.9972418
ATTAACC207.037954E-444.99668521
GCTAACA253.894535E-544.9961313
GCAGATA551.8189894E-1244.9961316
TCGGTTA302.168148E-644.9961316
CGATAAG207.0383813E-444.99612816
TAAGCGG302.1683336E-644.9955819
TATTACG850.044.99557520
CAACGTG351.2141209E-744.9944733
GCGAATC750.044.9944736
Produced by FastQC (version 0.11.5)