FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327192
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC82746624.86980011974061RNA PCR Primer, Index 13 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG803332.4144383612367424RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC525191.5784781882139653RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA293820.8830869994878565RNA PCR Primer, Index 1 (100% over 28bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTC250870.7539991680672471RNA PCR Primer, Index 13 (100% over 51bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC247690.7444415591285385RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG235620.708164722685075RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGT208610.6269851574540933RNA PCR Primer, Index 13 (100% over 46bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG205830.6186297634762287RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC174990.5259389899951671RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA148420.44608186122111376RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA143540.43141483869881875RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC133470.40114907705957453RNA PCR Primer, Index 13 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG118890.35732834173681594RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111490.3350873649612045RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG105960.31846674312753814RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA102310.307496531609838RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA101670.3055729876724878RNA PCR Primer, Index 1 (100% over 34bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT101500.30506204631412914RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC97460.2929196752096062RNA PCR Primer, Index 1 (100% over 31bp)
CTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCT97120.2918977924928889RNA PCR Primer, Index 13 (100% over 51bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG93520.28107785784529415RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC93080.27975542138836595No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATC92950.27936470152609166RNA PCR Primer, Index 13 (100% over 42bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCG89090.26776332715394846RNA PCR Primer, Index 13 (100% over 51bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA87430.2627741350664464RNA PCR Primer, Index 13 (100% over 40bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA85940.25829588433730305RNA PCR Primer, Index 1 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT83320.25042137634377576RNA PCR Primer, Index 8 (97% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC81170.24395947092924003RNA PCR Primer, Index 13 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG78730.2366259596680925RNA PCR Primer, Index 13 (100% over 45bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC78690.23650573817200812RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT78550.23608496293571277RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG77720.23359036689196175RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC76680.23046460799376772RNA PCR Primer, Index 13 (100% over 37bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG75540.2270382953553627RNA PCR Primer, Index 13 (100% over 45bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG75530.22700823998134165RNA PCR Primer, Index 2 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT74960.22529508366213913RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT74890.22508469604399145RNA PCR Primer, Index 1 (100% over 30bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA73970.22231960163405057RNA PCR Primer, Index 13 (100% over 38bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA73020.21946434110204643RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT73000.21940423035400422RNA PCR Primer, Index 13 (100% over 41bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA70670.21240132820708876RNA PCR Primer, Index 13 (100% over 39bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG68450.20572903517440533RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAATCTCGTATGCCG67830.20386560198509734RNA PCR Primer, Index 13 (97% over 39bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA66220.1990266867677008RNA PCR Primer, Index 13 (100% over 38bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG65890.19803485942500462RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG64810.19478887903072623RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA64610.19418777155030428RNA PCR Primer, Index 1 (100% over 34bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT63120.1897095208211609RNA PCR Primer, Index 13 (100% over 41bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC62830.1888379149745491RNA PCR Primer, Index 1 (100% over 33bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCT61260.18411922125323696RNA PCR Primer, Index 13 (100% over 51bp)
TCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTC57820.17378017258997977RNA PCR Primer, Index 13 (100% over 51bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT57480.1727582898732625RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC57120.17167629640850302RNA PCR Primer, Index 1 (100% over 31bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT57090.17158613028643974RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA56470.16972269709713175RNA PCR Primer, Index 13 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG56420.1695724202270263RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC53050.15944375918191678RNA PCR Primer, Index 1 (100% over 26bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT52800.15869237483138934RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC52210.15691910776414467RNA PCR Primer, Index 13 (100% over 44bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG51920.15604750191753286RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC49220.14793255093183683RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG48430.1455581763841702RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG47280.1421018083717441RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA47130.14165097776142765RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT47040.1413804793952378RNA PCR Primer, Index 8 (97% over 36bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC46970.1411700917770901RNA PCR Primer, Index 13 (98% over 51bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC46630.14014820906037284RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC45050.13539945996503958RNA PCR Primer, Index 1 (100% over 33bp)
TGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT44910.13497868472874425RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA44280.13308519616541517RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG43660.13122176297610716RNA PCR Primer, Index 2 (97% over 35bp)
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC42400.12743478584944903RNA PCR Primer, Index 13 (98% over 51bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG41970.12614240476654187RNA PCR Primer, Index 13 (100% over 45bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG40890.12289642437226346RNA PCR Primer, Index 2 (97% over 35bp)
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG40840.12274614750215798RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC40230.12091276968687109RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG39950.12007121921428039RNA PCR Primer, Index 2 (97% over 35bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA39010.11724601405629734RNA PCR Primer, Index 13 (100% over 39bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATG38840.11673507269793867RNA PCR Primer, Index 13 (100% over 49bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA38570.11592357759936907RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA36750.11045349952752953RNA PCR Primer, Index 13 (100% over 39bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36120.10856001096420044RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC35030.10528397519590092RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACAGTCAAATCTCGTATGCC34680.10423203710516254RNA PCR Primer, Index 13 (98% over 51bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA34320.10315004364040309RNA PCR Primer, Index 1 (100% over 34bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG34250.1029396560222554RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC34210.10281943452617101RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT34190.10275932377812882RNA PCR Primer, Index 13 (100% over 43bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG34020.10224838241977019Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAA600.045.263211
TTTAAGT351.15849616E-745.2632061
AGCTACA2500.045.2632061
CATCCGT206.835965E-445.2632061
TCCTTAT206.835965E-445.2632061
TCCCACA253.760631E-545.2632061
TCCTTCA116050.045.2242051
CATGTGC650.044.99654417
ACCCGTT207.0378795E-444.99654418
TATCACA502.1827873E-1144.9958720
TTATCGC406.8266672E-944.99586524
TATCGCC351.2137389E-744.99586525
TCGCACC650.044.9938414
TTCACTC750.044.9938415
TAACGAT900.044.9938414
TCAGGCC502.1827873E-1144.9938416
AGACGTT600.044.9938415
ACGATGT850.044.9938416
CTTTAAC1200.044.9938412
GTAACGA900.044.9938413
Produced by FastQC (version 0.11.5)