FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3441329
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG918992.6704508636053106RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC914562.657577929921841RNA PCR Primer, Index 16 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC911422.6484535480333324RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC842872.4492572491615885RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG758852.2051073872913634RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA606211.7615578167620705RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA533071.549023647550118No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG472571.37321947422057RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA297570.8646950059119602RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA294200.8549022775793887No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC266140.7733640114037339RNA PCR Primer, Index 5 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC263100.7645302149256872RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT259360.753662320574406RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG224160.6513762560917599RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC223580.64969086071108No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG201560.5857039533273337RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC194160.564200632953141RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG183630.5336019892314858RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG180840.5254946562795944No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC168860.49068252410623914RNA PCR Primer, Index 2 (100% over 34bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT167130.48565539650524553RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG158100.4594155339405212RNA PCR Primer, Index 1 (100% over 29bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC156150.453749118436511RNA PCR Primer, Index 2 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC155990.4532841817797717RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT150210.43648834505506445RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC149590.4346867155101997RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG148480.4314612174540708RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC148450.4313740418309322RNA PCR Primer, Index 1 (100% over 31bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC144830.42085484997220546RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG141280.4105390679008023No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC136440.3964747340344384RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT129690.37686021882824916RNA PCR Primer, Index 16 (100% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC129120.37520388198861543RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG128970.37476800387292236RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG120160.34916742921121463RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT116900.3396943448301514RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT116250.33780553966214794RNA PCR Primer, Index 1 (100% over 30bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC114240.3319647729118605RNA PCR Primer, Index 5 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT110050.3197892442135001RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA108180.3143552970378595RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC105430.30636419825015276RNA PCR Primer, Index 1 (100% over 31bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT103480.30069778274614256RNA PCR Primer, Index 16 (100% over 37bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG99860.2901785908874159RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG98270.28555828286106905Illumina Small RNA Adapter 2 (100% over 21bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT93200.27082560255064253RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT91040.26454895768466197RNA PCR Primer, Index 16 (100% over 37bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC89070.2588244250985593RNA PCR Primer, Index 5 (97% over 35bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG88550.25731338096415657RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA88480.2571099711768331RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG87460.2541459999901201RNA PCR Primer, Index 7 (97% over 36bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC87340.25379729749756563RNA PCR Primer, Index 2 (100% over 34bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG86320.25083332631085253No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA85280.24781123804204713No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA83730.24330716417988515RNA PCR Primer, Index 16 (100% over 40bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA82140.23868685615353835RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA80000.23246832836965023RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT74330.21599213559645125RNA PCR Primer, Index 1 (100% over 25bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC72380.21032572009244102RNA PCR Primer, Index 16 (100% over 38bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC72370.21029666155139484RNA PCR Primer, Index 1 (100% over 24bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAT71910.20895996866326935RNA PCR Primer, Index 16 (100% over 41bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG71690.2083206807602528RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA70970.20622846580492593RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC70890.2059959974765563RNA PCR Primer, Index 1 (100% over 33bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC68790.199893703856853RNA PCR Primer, Index 16 (100% over 38bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC67140.19509904458422894No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA66940.19451787376330482No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC66840.19422728835284275RNA PCR Primer, Index 5 (97% over 35bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC63230.1837371550351623RNA PCR Primer, Index 16 (100% over 39bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG62890.1827491646395913Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATC61710.17932025679613894RNA PCR Primer, Index 16 (100% over 42bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG59400.1726077338144653RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT58700.17057363594123084RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG58660.17045740177704602RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA58470.1699052894971681RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT58210.16914976742996674RNA PCR Primer, Index 16 (100% over 37bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG57410.16682508414627023RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA56570.16438416669838893No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC56190.16327994213863306RNA PCR Primer, Index 5 (97% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC56000.16272782985875514RNA PCR Primer, Index 1 (100% over 24bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG53950.15677082894428285RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG53690.1560153068770815Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT53220.1546495554479098RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG52940.15383591629861604RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT52530.15264451611572158RNA PCR Primer, Index 1 (100% over 25bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA51540.14976772055214715RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51400.14936090097750027RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG51360.14924466681331544Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT50560.14691998352961894RNA PCR Primer, Index 1 (100% over 30bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG49290.14322954881675073RNA PCR Primer, Index 7 (97% over 36bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA48310.14038181179422252RNA PCR Primer, Index 1 (100% over 32bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG47280.13738878206646327RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC46250.13439575233870404RNA PCR Primer, Index 2 (100% over 34bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG45270.13154801531617583Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG45240.13146083969303718Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC45070.13096684449525167RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC43260.12570724856588836RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC43210.12556195586065733RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG42720.12413808734939322No Hit
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA42390.12317915549486841RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA41740.121290350326865No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG41710.12120317470372638RNA PCR Primer, Index 7 (97% over 36bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA41560.12076729658803328RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC41380.12024424284920157RNA PCR Primer, Index 1 (100% over 31bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA41190.11969213056932365No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC40910.11887849142002987RNA PCR Primer, Index 1 (100% over 33bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG40680.11821014497596714RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC40060.11640851543110234RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG39870.11585640315122442RNA PCR Primer, Index 7 (97% over 36bp)
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA39030.1134154857033431RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG38950.11318301737497345RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA37820.10989940223675213No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG37730.10963787536733627No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA37420.10873706059490389RNA PCR Primer, Index 1 (100% over 32bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG37100.10780718728142528RNA PCR Primer, Index 7 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC37080.10774907019933287No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC37000.10751660187096322RNA PCR Primer, Index 2 (100% over 34bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA36830.10702260667317771RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG36350.10562779670295981RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA36090.10487227463575845RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC34750.10097843013556682RNA PCR Primer, Index 16 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGG302.0911139E-645.232921
TACCACA900.045.2329181
AAACCCG206.858577E-445.2329181
GGGGGTA700.045.2329181
GCTCCAA206.858577E-445.2329181
TCCTTCA120750.045.1018071
TGAGGTA92550.045.0618551
GTCTACG34250.045.0348171
CATTATT29300.045.001351
GAGTTTA551.8189894E-1244.99696418
ACATCGT302.1677752E-644.99696417
TATCGCC950.044.99696425
GCCCCAT253.8949867E-544.99500313
TACGCGT29750.044.99500316
GTGCTCG302.1684264E-644.99500313
GTAACGA2950.044.99500313
TCGTTAC207.040108E-444.99374
ACTGCCG1800.044.99375
ATAGGCG207.040108E-444.993711
CTATATT406.830305E-944.99373
Produced by FastQC (version 0.11.5)