FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572376
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC2651054.75748585522585RNA PCR Primer, Index 11 (100% over 51bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT853901.532380442382208RNA PCR Primer, Index 11 (100% over 37bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA615311.104214790961701RNA PCR Primer, Index 11 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT563441.0111306200443042RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG542850.974180493204335RNA PCR Primer, Index 6 (100% over 34bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA436610.7835257348032509RNA PCR Primer, Index 11 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC432900.7768678926188757RNA PCR Primer, Index 11 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG378060.6784538588207257RNA PCR Primer, Index 1 (100% over 29bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG333180.5979137086226773RNA PCR Primer, Index 2 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG303750.5450996128043046RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA301130.5403978482428321RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG296970.5329324510765245RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC284140.5099081612583214RNA PCR Primer, Index 1 (100% over 33bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT255210.4579913487532069RNA PCR Primer, Index 11 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA246780.44286315209167504RNA PCR Primer, Index 1 (100% over 28bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA242350.4349132219362082RNA PCR Primer, Index 11 (100% over 40bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA236250.42396636551445915RNA PCR Primer, Index 11 (100% over 38bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA226700.40682825423122915RNA PCR Primer, Index 11 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC213890.3838398557455563RNA PCR Primer, Index 1 (100% over 27bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA208940.374956750944301RNA PCR Primer, Index 11 (100% over 38bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG204720.3673836797804025RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC198890.35692135634781286RNA PCR Primer, Index 11 (100% over 39bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC198500.35622147536347154RNA PCR Primer, Index 11 (100% over 42bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT196510.3526502877767042RNA PCR Primer, Index 11 (100% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC195550.3509275038152486RNA PCR Primer, Index 1 (100% over 31bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT193020.3463872502501626RNA PCR Primer, Index 11 (100% over 41bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA187810.33703755812601305RNA PCR Primer, Index 1 (100% over 32bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC186350.33441749085129935RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC185520.33292800055129085RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC181380.3254984947175137RNA PCR Primer, Index 6 (97% over 36bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC176990.31762034722710747RNA PCR Primer, Index 1 (100% over 33bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG170620.30618895781619904RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT167160.2999797572884529RNA PCR Primer, Index 11 (100% over 37bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC163930.2941833070848055RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG160270.2876151932317561RNA PCR Primer, Index 11 (100% over 45bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC152800.2742097805316798RNA PCR Primer, Index 6 (97% over 36bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG143890.25822019188942025RNA PCR Primer, Index 6 (100% over 34bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA140230.25165207803637085RNA PCR Primer, Index 11 (100% over 38bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA138260.24811678178213387RNA PCR Primer, Index 1 (100% over 32bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC128900.23131963815794196RNA PCR Primer, Index 11 (100% over 51bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG125180.22464385030730158RNA PCR Primer, Index 2 (97% over 35bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG121560.21814751911931285RNA PCR Primer, Index 2 (97% over 35bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC112780.20239122413850033RNA PCR Primer, Index 11 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT112160.20127859283006028RNA PCR Primer, Index 1 (100% over 30bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC112110.20118886449873447RNA PCR Primer, Index 1 (100% over 33bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA110110.19759973124570201RNA PCR Primer, Index 11 (100% over 40bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG110070.19752794858064138RNA PCR Primer, Index 6 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT109820.1970793069240123RNA PCR Primer, Index 1 (100% over 30bp)
CACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC108510.19472842464327605RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC105910.19006255141433387RNA PCR Primer, Index 1 (100% over 33bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT101500.18214851259139728RNA PCR Primer, Index 11 (100% over 37bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT101500.18214851259139728RNA PCR Primer, Index 11 (100% over 41bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA97570.17509586574918848RNA PCR Primer, Index 1 (100% over 28bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG97260.17453955009496847RNA PCR Primer, Index 6 (100% over 34bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC95450.1712913845009741RNA PCR Primer, Index 11 (100% over 39bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT94570.1697121658696398RNA PCR Primer, Index 11 (100% over 41bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG94110.16888666522144233RNA PCR Primer, Index 11 (100% over 51bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG88660.1591062771069289RNA PCR Primer, Index 2 (97% over 35bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT88080.15806542846354948RNA PCR Primer, Index 11 (100% over 46bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC86080.154476295210517RNA PCR Primer, Index 1 (100% over 24bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG83520.1498822046466355RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG83290.14946945432253675RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC80500.14446261343455646RNA PCR Primer, Index 1 (100% over 31bp)
TACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC76940.13807395624415866RNA PCR Primer, Index 11 (100% over 39bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG75600.13566923696462693RNA PCR Primer, Index 2 (97% over 35bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT75510.13550772596824048RNA PCR Primer, Index 1 (100% over 25bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA74370.13346192001401197RNA PCR Primer, Index 11 (100% over 38bp)
TCCTTCATTCCACCGGACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG74320.13337219168268616RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG73280.1315058423911093RNA PCR Primer, Index 6 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG73160.13129049439592733RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG73140.131254603063397RNA PCR Primer, Index 2 (97% over 35bp)
CACTTGGACGAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT72720.1305008850802602RNA PCR Primer, Index 11 (100% over 37bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA72230.12962154743326723RNA PCR Primer, Index 1 (100% over 32bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA70540.12658872983445482RNA PCR Primer, Index 11 (100% over 38bp)
CTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCT69970.12556582685734058RNA PCR Primer, Index 11 (100% over 51bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT68480.1228919225838314RNA PCR Primer, Index 1 (100% over 30bp)
GTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC66830.1199308876500796RNA PCR Primer, Index 11 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA65560.117651788034404RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC64300.11539063408499355RNA PCR Primer, Index 6 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA60320.10824825891145896RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59270.10636396395361691RNA PCR Primer, Index 1 (100% over 33bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT58370.1047488539897523RNA PCR Primer, Index 11 (100% over 41bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA56200.1008546444102121RNA PCR Primer, Index 11 (100% over 38bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA56040.1005675137499695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA406.51562E-945.2347221
TACCTTA206.857599E-445.2347221
CGTGATA1300.044.99484312
ATGAGGT207.040224E-444.9940342
CCTATGA207.040224E-444.9940345
AGTAATT1100.044.9940345
ACACAGA253.895796E-544.994035
GACTTCA502.1827873E-1144.994034
CCGTTAC253.895796E-544.994034
TCCTTCA72450.044.860111
TGAGGTA5600.044.830841
ACATTCA9450.044.755972
CTTCATT75400.044.307783
CCTTCAT73500.044.2594382
TAGCTTA11350.044.2383651
TTCATTC78450.044.0763744
TCATTCC77850.044.0404055
AGCTTAT11450.044.011632
CATTCAA8550.043.9415443
CATTCCA77600.043.8923766
Produced by FastQC (version 0.11.5)