FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876478
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC88661015.087438428255837RNA PCR Primer, Index 10 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC1041091.7716223901459345RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA693271.1797372507818458No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG602031.0244741833458748RNA PCR Primer, Index 1 (100% over 29bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT601921.0242869963947794RNA PCR Primer, Index 10 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA598191.0179396570530852No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA591781.0070317629028815RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA578310.9841098698914554RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC565900.962991778408768RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT545390.9280899205272274RNA PCR Primer, Index 3 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA440830.7501602150131422RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG431150.7336877633167349RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT415010.7062223324923534RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG361170.614602828428865RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC342550.5829171827070568RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT331280.5637390287175413RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG316190.5380603824263445RNA PCR Primer, Index 7 (97% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC313680.5337891165422554RNA PCR Primer, Index 10 (100% over 37bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA311070.529347680702625RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG310150.527782117111644RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC294320.5008442131494408RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC284780.4846099993907916RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT283730.4828232148576069RNA PCR Primer, Index 1 (100% over 25bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC277250.4717962017385243RNA PCR Primer, Index 1 (100% over 31bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG276350.47026467213865175RNA PCR Primer, Index 1 (100% over 29bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC240480.4092247090859525RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT234390.3988613587934814RNA PCR Primer, Index 3 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC229010.3897062151853542RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA228850.389433943256488RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA226740.38584335719456453No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC212610.36179834247656506RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA209870.35713568569473075RNA PCR Primer, Index 7 (97% over 35bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA203130.3456662306912406RNA PCR Primer, Index 7 (97% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC197780.3365621380697758RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC189870.32310169458645127RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA185680.31597157344926674RNA PCR Primer, Index 10 (100% over 40bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA184810.3144910948360566RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC180190.306629242890044RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC176070.2996182407217384RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC173150.29464927801992963RNA PCR Primer, Index 1 (100% over 31bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT164540.2799976448478153RNA PCR Primer, Index 10 (100% over 39bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT163240.2777854354257771RNA PCR Primer, Index 3 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG160160.2725442007951021No Hit
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT159480.2713870450974206RNA PCR Primer, Index 3 (100% over 34bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA159070.27068934827970087RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA152920.26022389601390494RNA PCR Primer, Index 1 (100% over 23bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC152650.25976443713394315RNA PCR Primer, Index 1 (100% over 31bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA151790.2583009755162871RNA PCR Primer, Index 7 (97% over 35bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA150420.25596964712537RNA PCR Primer, Index 7 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC149880.2550507293654464No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT148840.2532809618278159RNA PCR Primer, Index 10 (100% over 38bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC148350.252447129045663RNA PCR Primer, Index 10 (100% over 37bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT142510.24250920364204545RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG136720.23265636321619854RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA136010.2314481565318546RNA PCR Primer, Index 1 (100% over 32bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG135920.23129500357186736RNA PCR Primer, Index 10 (100% over 51bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT134980.22969540598977825RNA PCR Primer, Index 10 (100% over 41bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133750.22760231553661903RNA PCR Primer, Index 1 (100% over 30bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC129010.21953625964395682RNA PCR Primer, Index 10 (100% over 42bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA124320.21155528872906526RNA PCR Primer, Index 10 (100% over 40bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT124150.21126599980464492RNA PCR Primer, Index 1 (100% over 25bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA122150.20786260069381693RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG121150.206160901138403RNA PCR Primer, Index 7 (97% over 36bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG120450.20496971144961318RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT119860.20396570871191894RNA PCR Primer, Index 3 (100% over 34bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC114440.1947424971215752RNA PCR Primer, Index 1 (100% over 24bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111740.19014790832195747RNA PCR Primer, Index 1 (100% over 31bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC111220.1892630245531422RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG109700.18667644122891297No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC106980.18204781843818696RNA PCR Primer, Index 10 (100% over 37bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT101800.17323301474114258RNA PCR Primer, Index 1 (100% over 30bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC101400.17255233491897698RNA PCR Primer, Index 10 (100% over 37bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT99860.16993171760363945RNA PCR Primer, Index 10 (100% over 39bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC96120.1635673612663912RNA PCR Primer, Index 10 (100% over 37bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC90170.15344224891167804RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT89150.1517065153651558RNA PCR Primer, Index 3 (100% over 34bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG87340.1486264391698565Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG85350.14524005705458268RNA PCR Primer, Index 7 (97% over 36bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA82320.14008390740167834RNA PCR Primer, Index 7 (97% over 35bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC81100.1380078339440733RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG80740.13739522210412428RNA PCR Primer, Index 10 (100% over 45bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC79750.13571053954426443RNA PCR Primer, Index 10 (100% over 50bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA78420.13344727913556384RNA PCR Primer, Index 7 (97% over 35bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG75850.12907391127814993RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT75050.12771255163381875RNA PCR Primer, Index 10 (100% over 41bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT74710.127133973784978RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT74650.12703187181165315RNA PCR Primer, Index 3 (100% over 34bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT73400.1249047473673857RNA PCR Primer, Index 10 (100% over 38bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT72330.12308392884309274RNA PCR Primer, Index 1 (100% over 30bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC68480.11653238555474894RNA PCR Primer, Index 1 (100% over 31bp)
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA67580.11500085595487637RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC66780.1136394963105452RNA PCR Primer, Index 10 (100% over 37bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA62360.10611798427561543RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG62240.10591378032896576RNA PCR Primer, Index 1 (100% over 29bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT59550.10133620852490216RNA PCR Primer, Index 10 (100% over 39bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG58890.10021308681832893RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACA2300.045.2427141
TCCTTCA87200.045.0351751
TATCGCC800.044.99671625
TGAGGTA27500.044.9959371
TACAGCT351.2140117E-744.99556717
GTATGCG207.0402544E-444.99403445
AACCGAC1950.044.99365210
CCTTAAT207.0405495E-444.9936522
AGATATA207.0405495E-444.99365211
ACCCGTA2900.044.9936522
TCAACGT351.2144119E-744.9936526
AAGCTGC6850.044.912481
CCTTCAT88000.044.686882
CTTCATT91050.044.6724433
GCTGCCC5900.044.61348712
GAGGTAG27950.044.5107152
GAGATGA37050.044.507892
AGATGAA36950.044.5065773
CACAGTG17150.044.468953
TTCATTC94300.044.4688074
Produced by FastQC (version 0.11.5)