FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307121
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26927811.67160283314139RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC473802.0536417465750607RNA PCR Primer, Index 33 (100% over 51bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA459041.9896659082900292RNA PCR Primer, Index 7 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC294981.2785631962952964RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA253471.098641987134615RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC227140.9845170669418726RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG213520.9254824519390183RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA203790.8833086777849969RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC193770.8398779257784919No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA169750.7357654843417402RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG167060.7241059311583571RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG164190.7116661848251565RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC143660.6226808216820877RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT122760.5320917281755053RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG109730.4756144129414972RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC104820.45433247757703216RNA PCR Primer, Index 1 (100% over 31bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC102330.44353980567122403RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG89980.39000988678097076RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG85960.37258557310171425RNA PCR Primer, Index 31 (97% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG82330.35685167791372885RNA PCR Primer, Index 31 (97% over 37bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCGTC77970.3379536660625949RNA PCR Primer, Index 33 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA69350.30059108299911447No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG66400.2878045841548839RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC63230.274064515905321RNA PCR Primer, Index 2 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG61310.26574245564060145RNA PCR Primer, Index 1 (100% over 29bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA60500.26223158646642286RNA PCR Primer, Index 33 (100% over 40bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA56360.24428714402062135RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG55720.2415131239323815RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT55690.2413830917407453RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG55370.23999608169662537RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA54140.23466476183953944RNA PCR Primer, Index 33 (100% over 40bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC53600.23232418239008704RNA PCR Primer, Index 2 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCG51760.2243488746363975RNA PCR Primer, Index 33 (100% over 51bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG48340.20952520478986583RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA47990.20800816255410964No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA46460.20137652078066123No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG46120.19990282260878384RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG45260.19617523311521154RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC42830.1856426255926759RNA PCR Primer, Index 1 (100% over 31bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG42630.18477574431510094Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG42590.18460236805958594Illumina Small RNA Adapter 2 (100% over 21bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG41450.17966114477740872RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC40970.17758062971122884No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG40660.17623696373098768Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC40500.1755434587089277RNA PCR Primer, Index 1 (100% over 24bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA39930.1730728470678391RNA PCR Primer, Index 1 (100% over 22bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT39630.17177252515147667RNA PCR Primer, Index 1 (100% over 30bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG39440.17094898793778046RNA PCR Primer, Index 31 (97% over 37bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA38400.16644120529439071RNA PCR Primer, Index 7 (100% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG37920.16436069022821084RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA37160.16106654137342602RNA PCR Primer, Index 1 (100% over 23bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC36390.15772904845476246RNA PCR Primer, Index 33 (100% over 38bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA36280.15725226375209622RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG36190.15686216717718748RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA36090.15642872653840004No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT35710.1547816521110076RNA PCR Primer, Index 1 (100% over 30bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA35500.15387142676955393RNA PCR Primer, Index 33 (100% over 40bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG34850.15105406261743531RNA PCR Primer, Index 31 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT34610.1500138050843454RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC34490.14949367631780042RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG34470.14940698819004292RNA PCR Primer, Index 7 (100% over 36bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCG34380.14901689161513418RNA PCR Primer, Index 33 (100% over 39bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC33520.14528930212156188No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC33370.1446391411633807No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA32990.14299206673598827RNA PCR Primer, Index 1 (100% over 32bp)
CTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCT32740.14190846513901959RNA PCR Primer, Index 33 (100% over 51bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA31940.1384409400287198RNA PCR Primer, Index 1 (100% over 28bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC31610.13701058592072113RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC30710.13310962017163383No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA30500.13219939483018012No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG30370.1316359219997564No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCG30310.13137585761648393RNA PCR Primer, Index 33 (100% over 39bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC30280.13124582542484767RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG30280.13124582542484767RNA PCR Primer, Index 1 (100% over 29bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC30210.13094241697769643RNA PCR Primer, Index 2 (100% over 34bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA29560.12812505282557785No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG29460.12769161218679037RNA PCR Primer, Index 7 (100% over 36bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG29450.12764826812291163RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC29200.12656466652594295RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG28610.12400736675709682RNA PCR Primer, Index 7 (100% over 36bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT28460.12335720579891563RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC27850.12071321790231201RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTC27660.1198896806886158RNA PCR Primer, Index 33 (100% over 44bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC27350.11854601470837464RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC27190.11785250968631468RNA PCR Primer, Index 1 (100% over 26bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG27130.11759244530304218No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC26910.11663887589770976RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA26710.1157719946201348RNA PCR Primer, Index 7 (100% over 35bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATC26480.11477508115092361RNA PCR Primer, Index 33 (100% over 42bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA26270.11386485580946991RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA26270.11386485580946991RNA PCR Primer, Index 1 (100% over 28bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC25790.11178434074329002RNA PCR Primer, Index 1 (100% over 31bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT25730.11152427636001754RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG25030.10849019188850519Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG24780.1074065902915365RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC24610.1066697412055978No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC24580.10653970901396156RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT24500.10619295650293158RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC24430.10588954805578034RNA PCR Primer, Index 1 (100% over 27bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC24050.10424247362838793RNA PCR Primer, Index 33 (100% over 38bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC23990.10398240924511545RNA PCR Primer, Index 2 (100% over 34bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA23650.10250871107323803RNA PCR Primer, Index 7 (100% over 35bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC23500.10185855011505682RNA PCR Primer, Index 33 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG406.513801E-945.235041
TACCTTA206.856521E-445.235041
TCATACG206.856521E-445.235041
CACGCTC351.1639713E-745.2350351
TGTGCAA351.1639713E-745.2350351
TAGTTTA850.045.2350351
TCGTACA253.774118E-545.2350351
ACGTACG502.0008883E-1145.2350351
GCAGCGA351.1639713E-745.2350351
AGCTACA3000.045.2350351
GCATCGT253.774118E-545.2350351
GTCTACG37950.045.1754381
ATTCGGT207.0342317E-445.00069424
TACGCGC207.0342317E-445.00069422
AGGCATC253.8916773E-545.00069422
CATCAGC253.8916773E-545.00069425
CAAACCC302.1678425E-644.9958215
TAACGAT302.1678425E-644.9958214
TAACCTG900.044.9958215
GATAGGG502.1827873E-1144.9958215
Produced by FastQC (version 0.11.5)