FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8123647
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1330581.6379096728353657RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1167251.4368546540734721RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA1118881.3773124312270093RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC932841.1483019880110497RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA784730.965982396822511RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA761140.9369437150580275RNA PCR Primer, Index 39 (100% over 38bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT688220.8471810751993532RNA PCR Primer, Index 2 (97% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC683090.8408661774693066RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG641670.789879225426708RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC635800.7826534067765376RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC469640.5781147309822793RNA PCR Primer, Index 1 (100% over 31bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC447270.5505778377617836RNA PCR Primer, Index 2 (100% over 34bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG428830.5278786732116746RNA PCR Primer, Index 1 (100% over 29bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC393230.484055991108427RNA PCR Primer, Index 39 (100% over 39bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT383820.4724725237322596RNA PCR Primer, Index 1 (97% over 35bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT356510.4388546178828302RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC355180.43721742217504034RNA PCR Primer, Index 1 (100% over 31bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG335540.4130410885652713RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT331330.40785868711429735RNA PCR Primer, Index 2 (97% over 37bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA307840.37894310277145227RNA PCR Primer, Index 39 (100% over 40bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC303160.37318214343877815RNA PCR Primer, Index 1 (100% over 31bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC269760.3320676046115741RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT267720.32955641721015205RNA PCR Primer, Index 39 (100% over 41bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG250700.30860523604730733RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC242420.29841276953565316RNA PCR Primer, Index 1 (100% over 33bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA239820.2952122365730564RNA PCR Primer, Index 39 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC234820.28905736549113964RNA PCR Primer, Index 39 (100% over 51bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT223320.274901162002731RNA PCR Primer, Index 2 (97% over 37bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA218000.26835237917157156RNA PCR Primer, Index 2 (97% over 36bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT211300.2601048519218031RNA PCR Primer, Index 39 (100% over 41bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA211250.2600433032109839RNA PCR Primer, Index 1 (100% over 32bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA210780.25946474532928376RNA PCR Primer, Index 39 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG202880.24974004901985528RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA197820.24351131948495544RNA PCR Primer, Index 2 (97% over 36bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA196920.24240344269021047RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG196340.24168947764470808RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA193450.2381319621593602RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA192870.23741799711385786RNA PCR Primer, Index 1 (100% over 23bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC190950.23505452661840182RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA190590.2346113759005038RNA PCR Primer, Index 39 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG189510.23328192374680976RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC172920.21286006149700992RNA PCR Primer, Index 2 (100% over 34bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC171880.2115798483119712RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC170420.20978262595605152RNA PCR Primer, Index 1 (100% over 24bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA169350.2084654835445213RNA PCR Primer, Index 39 (100% over 38bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC166880.20542497723005446RNA PCR Primer, Index 2 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT160940.19811299038473731RNA PCR Primer, Index 1 (100% over 30bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC159100.19584799782659193No Hit
CACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC155580.1915149685849225RNA PCR Primer, Index 1 (100% over 33bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC153800.18932383447976012RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA153050.18840060381747262No Hit
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAT151360.18632025739178476RNA PCR Primer, Index 2 (97% over 37bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA148410.18268888345345385RNA PCR Primer, Index 39 (100% over 40bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT145050.17855281008640578RNA PCR Primer, Index 1 (97% over 35bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATC143670.17685406566779674RNA PCR Primer, Index 39 (100% over 42bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT141910.17468755104696204RNA PCR Primer, Index 1 (97% over 35bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC138460.17044069000043946RNA PCR Primer, Index 1 (100% over 33bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT137050.1687050163553389RNA PCR Primer, Index 39 (100% over 41bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC134250.16525828854946553RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA132720.163374897998399No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA131540.16192234842306663RNA PCR Primer, Index 1 (100% over 28bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACAT129720.15968197534924894RNA PCR Primer, Index 39 (100% over 41bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATC128470.15814325757876974RNA PCR Primer, Index 39 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTC126530.155755167598986RNA PCR Primer, Index 39 (100% over 44bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG124860.1536994406576258Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT124660.15345324581434913RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC123960.15259156386288078RNA PCR Primer, Index 39 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG121250.1492556237364819No Hit
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA118060.145328815986219RNA PCR Primer, Index 39 (100% over 38bp)
TCGTGGTTGTAGCCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA115210.14182053946952644RNA PCR Primer, Index 1 (100% over 22bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC114790.14130353029864542RNA PCR Primer, Index 39 (100% over 39bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC113610.13985098072331306RNA PCR Primer, Index 39 (100% over 39bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC113100.13922318387295754RNA PCR Primer, Index 2 (100% over 34bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT112190.1381029973360487RNA PCR Primer, Index 1 (97% over 35bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA111270.136970501056976RNA PCR Primer, Index 1 (100% over 32bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATA110600.13614574833199916RNA PCR Primer, Index 39 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA110460.13597341194170548No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC107930.1328590471742556No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA105660.13006473570306537RNA PCR Primer, Index 1 (100% over 22bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC103580.127504309332988RNA PCR Primer, Index 1 (100% over 31bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT103390.12727042423187515RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG101240.12462382966665095RNA PCR Primer, Index 1 (100% over 29bp)
GTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC98730.12153408438352874RNA PCR Primer, Index 39 (100% over 39bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC95390.11742263050080833RNA PCR Primer, Index 39 (100% over 39bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG93970.11567464711354396RNA PCR Primer, Index 1 (100% over 29bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA93650.11528073536430127No Hit
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC92570.11395128321060725RNA PCR Primer, Index 1 (100% over 27bp)
CTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC91740.11292957461100908RNA PCR Primer, Index 39 (100% over 39bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG91130.11217868033901522RNA PCR Primer, Index 1 (100% over 29bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATAC87970.10828880181524382RNA PCR Primer, Index 39 (100% over 39bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT83920.10330335623889121RNA PCR Primer, Index 1 (97% over 35bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG81510.10033670837740734No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACA81310.10009051353413066RNA PCR Primer, Index 39 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC453.8562575E-1044.993655
GTCTACG182200.044.951191
TGAGGTA39600.044.8940121
TCCTTCA50050.044.8299941
TTCACAG32200.044.8152581
AGCTTAT71150.044.8039362
TAGCTTA71600.044.636511
CAACGGA6500.044.5407641
TCAAGTA21650.044.4740942
CTTCATT50650.044.4162373
TTCATTC52500.044.393734
GCTTATC70050.044.3834533
GAGATGA69450.044.3781852
GCTACAT3200.044.2906232
GAGGTAG39750.044.2579042
CAACGCT11600.044.2178967
TCATTCC51450.044.2065855
AAGTAAT21650.044.162364
ACATTCA15200.044.1056142
CATTCCA51250.044.0718276
Produced by FastQC (version 0.11.5)