FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779715
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC866164.866846658032325RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG342101.9222178832004RNA PCR Primer, Index 2 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC285691.605257021489396RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA251461.4129228556257603RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA188901.061405899259151RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG182271.0241527435572548RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT149690.8410897250402453RNA PCR Primer, Index 1 (100% over 30bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA143810.8080507272231789RNA PCR Primer, Index 22 (100% over 37bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA136150.7650101280261166RNA PCR Primer, Index 22 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG116840.6565096096846967RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC111000.6236953669548214No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC108100.6074006231334792RNA PCR Primer, Index 22 (100% over 51bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG89480.5027771300461029RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC89040.5003048240870026RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG72290.4061886313258022RNA PCR Primer, Index 22 (100% over 39bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC69080.3881520355787303RNA PCR Primer, Index 22 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG62330.35022461461526144RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA61940.34803325251514994RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC60600.34050395709425385RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG59620.33499745745807613RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG58030.3260634427422368RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC54870.3083077908541536RNA PCR Primer, Index 22 (100% over 38bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC53080.29825000070235963No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG50740.28510182810169044RNA PCR Primer, Index 2 (100% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50100.28150574670663564RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTC49450.27785347653978304RNA PCR Primer, Index 22 (100% over 51bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC46930.2636939060467547RNA PCR Primer, Index 1 (100% over 24bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG45050.25313041694878113RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA44650.2508828660768719RNA PCR Primer, Index 1 (100% over 23bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA44090.24773629485619889No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA43710.24560112152788507No Hit
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA43600.24498304503811005RNA PCR Primer, Index 1 (100% over 28bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA43380.24374689205855996RNA PCR Primer, Index 22 (100% over 40bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC42740.2401508106635051RNA PCR Primer, Index 1 (100% over 31bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA40600.22812641349879054RNA PCR Primer, Index 22 (100% over 37bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC40540.22778928086800415RNA PCR Primer, Index 2 (100% over 34bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA39990.22469889841912888RNA PCR Primer, Index 1 (100% over 22bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA39970.22458652087553344RNA PCR Primer, Index 22 (100% over 37bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC39190.22020379667531037RNA PCR Primer, Index 2 (100% over 34bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC39180.22014760790351265RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC39170.22009141913171493RNA PCR Primer, Index 2 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC38940.21879907738036708RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT38380.21565250615969409RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC38010.21357352160317802RNA PCR Primer, Index 1 (100% over 24bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT37410.21020219529531411RNA PCR Primer, Index 12 (97% over 36bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC35750.20087485917689069RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC35720.2007062928614975RNA PCR Primer, Index 1 (100% over 27bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG33440.18789525289161468RNA PCR Primer, Index 2 (100% over 35bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC31910.17929837080656172RNA PCR Primer, Index 1 (100% over 31bp)
CTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCT31660.17789365151161843RNA PCR Primer, Index 22 (100% over 51bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC31420.17654512098847286RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA30590.17188145292926113RNA PCR Primer, Index 22 (100% over 37bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT30530.17154432029847477RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA30080.16901582556757683No Hit
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG29310.16468929013915148RNA PCR Primer, Index 22 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA29290.164576912595556No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA29170.16390264733398324No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT28460.1599132445363443RNA PCR Primer, Index 12 (97% over 36bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC28360.15935135681836698RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA28110.1579466375234237RNA PCR Primer, Index 22 (100% over 40bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG28030.15749712734904184No Hit
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC27550.15480006630275073RNA PCR Primer, Index 2 (100% over 34bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG27280.15328296946421197Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA26900.15114779613589815No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG26500.1489002452639889No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT26120.1467650719356751RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26040.14631556176129323RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG24510.1377186796762403RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC24340.13676347055567886No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTC24260.136313960381297RNA PCR Primer, Index 22 (100% over 51bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG23930.13445973091197186Illumina Small RNA Adapter 2 (100% over 21bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC23790.13367308810680362RNA PCR Primer, Index 2 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC23760.13350452179141042No Hit
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA23440.13170648109388303RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC23400.13148172600669208RNA PCR Primer, Index 2 (100% over 34bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA23250.13063889442972612RNA PCR Primer, Index 1 (100% over 32bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC23220.1304703281143329RNA PCR Primer, Index 22 (100% over 38bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC23190.1303017617989397RNA PCR Primer, Index 1 (100% over 27bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG23110.12985225162455788RNA PCR Primer, Index 2 (100% over 35bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23050.12951511899377147RNA PCR Primer, Index 1 (100% over 31bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC23030.129402741450176RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG22960.1290094200475919No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA22910.12872847618860322RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA22720.12766088952444632RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC22700.12754851198085088RNA PCR Primer, Index 2 (100% over 34bp)
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT22460.1261999814577053RNA PCR Primer, Index 22 (100% over 41bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG22250.12502001724995293Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC22090.12412099690118923RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC21700.1219296348010777RNA PCR Primer, Index 1 (100% over 24bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA21590.12131155831130265RNA PCR Primer, Index 22 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA21540.121030614452314No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG21410.12030016041894348RNA PCR Primer, Index 1 (100% over 29bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC21300.11968208392916843RNA PCR Primer, Index 22 (100% over 38bp)
CAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC21050.11827736463422514RNA PCR Primer, Index 22 (100% over 38bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG21040.11822117586242742RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA21010.11805260954703423RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG20900.11743453305725916RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG20750.11659170148029319RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC20400.11462509446737258No Hit
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT20090.1128832425416429RNA PCR Primer, Index 12 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC20060.11271467622624971No Hit
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT20050.11265848745445198RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT19520.10968048254917219RNA PCR Primer, Index 1 (100% over 30bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG19480.10945572746198128Illumina Small RNA Adapter 2 (100% over 21bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA19460.1093433499183858RNA PCR Primer, Index 22 (100% over 37bp)
ATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC19390.1089500285158017RNA PCR Primer, Index 22 (100% over 38bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC18760.1054101358925446RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC18280.10271307484625349RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG18060.10147692186670337RNA PCR Primer, Index 2 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA17990.10108360046411925RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph