FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884380
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1318476.996837155987645RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC830674.408187308292383RNA PCR Primer, Index 43 (100% over 51bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTC486552.5820163661257287RNA PCR Primer, Index 43 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA445002.3615194387543914RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG375901.9948205775905072RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA282371.4984769526316348RNA PCR Primer, Index 7 (97% over 35bp)
CTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCT246111.3060529192625692RNA PCR Primer, Index 43 (100% over 51bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG215741.1448858510491515RNA PCR Primer, Index 1 (100% over 29bp)
TCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTC176200.9350555620416263RNA PCR Primer, Index 43 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG150680.7996264023180037RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC124150.6588373894861971RNA PCR Primer, Index 1 (100% over 31bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCT119780.6356467379191034RNA PCR Primer, Index 43 (100% over 51bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC114020.605079654846687RNA PCR Primer, Index 1 (100% over 31bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC108750.5771128965495282RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA99520.5281312686400832No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG96730.5133253377768815RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG81900.4346257124359205RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT78490.41652957471422963RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT77460.41106358590093295RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG73900.39217143039089775RNA PCR Primer, Index 1 (100% over 29bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT63630.33767074581559986RNA PCR Primer, Index 1 (100% over 30bp)
TTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTT58380.3098101232235536RNA PCR Primer, Index 43 (100% over 51bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT56820.3015315382247742RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTC53830.28566425031044695RNA PCR Primer, Index 43 (100% over 44bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51400.27276876213927126RNA PCR Primer, Index 1 (100% over 33bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC50760.26937241957566943RNA PCR Primer, Index 43 (100% over 42bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG50210.2664536876850741No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG49370.26199598807034674RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA48900.25950179900020165No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG48550.2576444241607319Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCG47760.25345206380878593RNA PCR Primer, Index 43 (100% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG47130.25010878909774037RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA47100.24994958554007154RNA PCR Primer, Index 43 (100% over 40bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGT45140.23954828643904094RNA PCR Primer, Index 43 (100% over 46bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT44730.23737250448423353RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT44430.2357804689075452RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC44240.23477217970897588RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG43400.2303144800942485RNA PCR Primer, Index 43 (100% over 45bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA42540.22575064477440857RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG42040.22309725214659462RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC40660.21577388849382823RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA39270.2083974569885055No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA38210.20277226461753997RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA37070.19672252942612425No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG34900.18520680542141182Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT34520.18319022702427323RNA PCR Primer, Index 3 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC34320.18212886997314767RNA PCR Primer, Index 1 (100% over 27bp)
TCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCTG33370.1770874239803012RNA PCR Primer, Index 43 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC32610.17305426718602404No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA32370.17178063872467336No Hit
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT32030.1699763317377599RNA PCR Primer, Index 43 (100% over 41bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT31770.16859656757129668RNA PCR Primer, Index 3 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA31730.16838429616107153RNA PCR Primer, Index 7 (97% over 35bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA31430.1667922605843832RNA PCR Primer, Index 43 (100% over 40bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGC31210.1656247678281451RNA PCR Primer, Index 43 (100% over 39bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA31200.1655716999755888RNA PCR Primer, Index 43 (100% over 37bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG31140.16525329286025112RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG30940.16419193580912556RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA30890.16392659654634417RNA PCR Primer, Index 43 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG30540.1620692217068744RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA30390.16127320391853023RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC29690.15755845423959075RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG29420.15612562222057122RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG28560.15156178690073127RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG28210.14970441206126153Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG28110.14917373353569877RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATG27570.1463080694976597RNA PCR Primer, Index 43 (100% over 49bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG27180.14423842324796485RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT26850.14248718411360767RNA PCR Primer, Index 43 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA26600.1411604877997007No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC26550.1408951485369193RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA25680.1362782453645231No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG25330.13442087052505333RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG24970.1325104278330273RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT24770.13144907078190174RNA PCR Primer, Index 3 (100% over 34bp)
ACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC24760.13139600292934547RNA PCR Primer, Index 43 (100% over 42bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAG24750.13134293507678918RNA PCR Primer, Index 43 (100% over 38bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC24060.12768125325040597RNA PCR Primer, Index 43 (100% over 50bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23960.12715057472484317RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG23820.1264076247890553RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA23670.1256116070007111RNA PCR Primer, Index 7 (97% over 35bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA23370.12401957142402277RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23250.12338275719334742RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA23160.12290514652034092RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGG22480.11929653254651397Illumina Small RNA Adapter 2 (100% over 21bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAC22340.11855358261072607RNA PCR Primer, Index 30 (97% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG22260.11812903979027585No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA22200.11781063267493819RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT21660.11494496863689915RNA PCR Primer, Index 3 (100% over 34bp)
TCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC21440.113777475880661RNA PCR Primer, Index 43 (100% over 42bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG21340.11324679735509822RNA PCR Primer, Index 43 (100% over 45bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT21340.11324679735509822RNA PCR Primer, Index 43 (100% over 41bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC21040.11165476177840987RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC20480.10868296203525828RNA PCR Primer, Index 1 (100% over 24bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC19810.10512741591398762RNA PCR Primer, Index 1 (100% over 27bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT19120.10146573408760441RNA PCR Primer, Index 3 (100% over 34bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA18940.10051051274159141RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACA1400.045.2334251
ATCTAAA4100.045.233421
TCCCTAG253.774649E-545.233421
AATGGCG253.774649E-545.233421
TAACATT253.774649E-545.233421
TACTAGA253.774649E-545.233421
TCTTTCA950.045.233421
GCAGCGA551.8189894E-1245.233421
AGCTACA2000.045.233421
AGTAAGG850.045.233421
TACCCAT206.857405E-445.2334181
TTCAGGG302.0904445E-645.2334181
TAGGTAG406.513801E-945.2334181
TGTGCAA406.513801E-945.2334181
ACCAGGA650.045.2334181
CTTTCAG406.513801E-945.2334181
AGAGGTA206.857405E-445.2334181
TCCTTCA25400.045.1443751
GTCTACG26600.045.063371
TGAGGTA34250.045.0353161
Produced by FastQC (version 0.11.5)