FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140989
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC1195223.8052345933080316RNA PCR Primer, Index 40 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1089043.4671882009137884RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA474341.5101612899631296RNA PCR Primer, Index 1 (97% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT386111.2292625029887083RNA PCR Primer, Index 1 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC354331.128084179855453No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC351861.1202204146528372RNA PCR Primer, Index 1 (100% over 31bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTC326341.0389721199278317RNA PCR Primer, Index 40 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC315721.0051611132671907RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG297660.9476632996804509RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA219300.6981877364104109RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC175510.558773048870913RNA PCR Primer, Index 1 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA163510.5205685215707536No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA160460.5108582042152966RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA158610.5049683395898553RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG149910.4772700572972398RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT143860.4580086081167428RNA PCR Primer, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG143470.45676696097948766RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC142000.45208690638521815RNA PCR Primer, Index 2 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCG138440.44075289661950423RNA PCR Primer, Index 40 (100% over 51bp)
CTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTCT135140.4302466516119604RNA PCR Primer, Index 40 (100% over 51bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG121080.38548368045860715RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG119980.3819815987894259RNA PCR Primer, Index 40 (100% over 45bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG99410.31649267157573613RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA96880.30843788373661923RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA94610.30121086065567243No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA89640.28538781893218984No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGT86340.274881573924646RNA PCR Primer, Index 40 (100% over 46bp)
TCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTTC85680.27278032492313725RNA PCR Primer, Index 40 (100% over 51bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA85620.27258930228663647RNA PCR Primer, Index 1 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC85170.27115663251288047No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC83170.26478921129618727RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC82140.2615099893695903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC80510.2563205410779853No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG80210.25536542789548133RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT78700.25055802487687795RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA78580.2501759796038764RNA PCR Primer, Index 1 (100% over 28bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCT78440.24973026011870783RNA PCR Primer, Index 40 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA76760.24438162629668556No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG76230.24269425967426184RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT75560.24056117356666962RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC74790.2381097163982427RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG74520.23725011453398914RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC74290.23651786109406941No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC72780.23171045807546606RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC70540.22457894631276965RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG68700.21872091879341188RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT66240.21088899069687922RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG64530.20544484555660653RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG62250.1981859853695763RNA PCR Primer, Index 40 (100% over 45bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT62250.1981859853695763RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA61510.19583003951939978No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC61150.19468390370039498RNA PCR Primer, Index 1 (97% over 36bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG61100.19452471816997768RNA PCR Primer, Index 40 (100% over 45bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA59370.18901689881753805RNA PCR Primer, Index 1 (100% over 22bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT57810.18405031026851734RNA PCR Primer, Index 40 (100% over 41bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC56490.1798478122654998RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC53480.17026484333437653RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA53390.16997830937962533No Hit
GCCGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC52830.16819543143895124RNA PCR Primer, Index 40 (100% over 42bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAG52180.16612601954352596RNA PCR Primer, Index 40 (100% over 38bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG52120.16593499690702515RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG51960.1654256032096897RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTC51890.16520274346710542RNA PCR Primer, Index 40 (100% over 44bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC50580.16103208257017138RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC50400.16045901466066897RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG49870.15877164803824528RNA PCR Primer, Index 40 (100% over 45bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC49770.15845327697741063No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC49650.158071231704409RNA PCR Primer, Index 1 (100% over 33bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC49390.15724346694623892No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC49120.15638386508198532RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA49010.1560336569150672RNA PCR Primer, Index 40 (100% over 40bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA48980.1559381455968168RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA48360.1539642450196419RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG48190.15342301421622298RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC48100.15313648026147178RNA PCR Primer, Index 40 (100% over 42bp)
GGAATACCGGGTGCTGTAGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA47990.15278627209455367RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG47060.14982542122879133RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA45400.14454046161893594No Hit
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCG44840.14275758367826183RNA PCR Primer, Index 40 (100% over 45bp)
GAATACCGGGTGCTGTAGGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA44670.14221635287484294RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC44610.14202533023834213RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA44080.14033796361591844RNA PCR Primer, Index 1 (100% over 32bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT43660.13900080516041285RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG43090.1371860901136553RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCA42820.1363264882494017RNA PCR Primer, Index 1 (97% over 37bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC42550.13546688638514812RNA PCR Primer, Index 1 (100% over 33bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC42070.13393870529314175RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC41950.13355666002014013No Hit
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT41500.1321239902463842RNA PCR Primer, Index 1 (100% over 30bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC41260.131359899700381RNA PCR Primer, Index 1 (100% over 24bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA40890.13018192677529275RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG40010.12738026143994774Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCTT39830.12680719353044534RNA PCR Primer, Index 40 (100% over 51bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC39770.12661617089394456RNA PCR Primer, Index 1 (97% over 36bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC39510.12578840613577444RNA PCR Primer, Index 1 (100% over 24bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA39260.12499247848368779RNA PCR Primer, Index 40 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG39010.12419655083160112No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG38730.12330511186126407No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG38320.12199979051184197RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC38220.1216814194510073RNA PCR Primer, Index 2 (100% over 34bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCT37680.11996221572250015RNA PCR Primer, Index 40 (100% over 43bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT36670.11674666800807007RNA PCR Primer, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT36550.11636462273506847RNA PCR Primer, Index 1 (100% over 30bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC36470.11610992588640076RNA PCR Primer, Index 2 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36280.11550502087081489RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA36070.11483644164306211No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT35850.11413602530922584RNA PCR Primer, Index 1 (100% over 30bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC35730.11375398003622425RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG34930.11120701154954697RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAAT33850.10776860409253262RNA PCR Primer, Index 40 (100% over 41bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGTG33840.10773676698644917No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33810.10764125566819878RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG33740.1074183959256145RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA33520.10671797959177826No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT33480.1065906311674444RNA PCR Primer, Index 1 (97% over 35bp)
CTCGGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATC33270.10592205193969162RNA PCR Primer, Index 40 (100% over 42bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG32860.1046167305902695RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG32370.10305671239217967No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC31600.10060525522375277RNA PCR Primer, Index 2 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC31450.10012769863250078RNA PCR Primer, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAG551.8189894E-1245.220651
TTCACAT206.8676594E-445.2206461
GTTCACA302.0949647E-645.2206461
TCCCATA302.0949647E-645.2206461
GCCCGAC206.8676594E-445.2206461
CCACACG206.8676594E-445.2206461
TGAGGTA56050.045.099631
TCCTTCA56100.045.059431
CAACGGA10450.045.004281
CTTCGCC207.034346E-445.00105326
ATTACGT253.891819E-545.0010521
TTACGTT253.891819E-545.0010522
TATCACT900.044.99603720
TATTACG253.8943814E-544.99603720
CACACTT2000.044.99603719
GCCAGTA207.038761E-444.99532316
GCATAGT351.2138116E-744.99532315
ATCAGGC750.044.9953215
CTCGTAA551.8189894E-1244.9938942
GTAAGCC551.8189894E-1244.9938945
Produced by FastQC (version 0.11.5)