FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221163
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA75881614.533466968949254No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA4930479.443240902457939No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC1377302.637918027075577No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC1342022.570346874824632No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA1300732.49126487719307No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC1300612.491035043341876No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG999471.914266993771311No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG902201.7279675045578928No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC843661.6158468908172376No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA827711.5852981414294094No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC757171.4501941425693854No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA745631.4280917872129255No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA702461.3454090592459955No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA646221.2376935943198861No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG569531.0908106105861854RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC544611.0430817808216293RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA537461.0293875138546718No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA533041.0209219670023708No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG412040.7891728337154001RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG396770.7599264761510032RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC393820.7542763939758249RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA372270.7130020648656248No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG342120.6552563097532101RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG315150.6036011516974283Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA305290.5847164702576801No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA284470.5448402970755749No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA276630.5298244854642539No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC276390.5293648177618664No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG266750.51090149838264RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA265560.5086223126916359No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC256300.4908868005078562RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA249160.47721168636183164No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT240490.4606061906130875RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA192280.3682704408960226No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG190420.3647080162025204RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA189850.36361630540935036No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG186820.35781300066670974Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA184330.35304394825444063RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG182460.34946237074000563RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG154880.29663889060732257No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA145240.2781755712280961RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG142340.27262125315758196RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG134550.2577012056509249RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT133240.2551921861087271No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA131960.25274062502932776No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC129240.2475310577356041No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA128780.24665002797269497RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA128390.2459030679563155RNA PCR Primer, Index 1 (100% over 28bp)
TGAAATGTTTAGGACCACTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG122500.23462205642689185RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA117020.22412631055571336No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG115540.22129169305765786RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC109230.2092062630490563RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA108750.20828692764428156RNA PCR Primer, Index 7 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGAATTCTCGGGTGCCA102610.19652709559153775No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA101890.19514809248437562No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGCC96790.18538015380864378No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC96720.18524608406211412No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA93830.17971091881253276RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG87190.16699344571314859RNA PCR Primer, Index 1 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG82730.15845128757711643No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC82210.15745534088861043RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG81900.1568616034396934RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC66800.12794084383115412RNA PCR Primer, Index 10 (100% over 51bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC64750.12401451553992855RNA PCR Primer, Index 1 (100% over 27bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC64190.12294195756769133No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG63500.12162041292332762RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA63380.12139057907213392No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA63110.12087345290694813No Hit
GTTTCCGTCGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA61230.11727272257158032No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG60470.11581710818068694No Hit
TGAAATGTTTAGGACCACTAGATGGAATTCTCGGGTGCCAAGGAACTCCAG59810.11455302199912165RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG59640.11422742404326393RNA PCR Primer, Index 7 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTTGGAATTCTCGGGTGC59110.11321232453382513No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG59010.11302079632449706RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA58880.11277180965237055No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA57340.10982227522871821RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC57280.10970735830312137RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC56220.10767715928424375RNA PCR Primer, Index 1 (100% over 27bp)
TGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT53950.10332946893249646RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG53410.10229521660212486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)