FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520358
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1975075.6104237125883225RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC482511.3706276463927816RNA PCR Primer, Index 38 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA415821.1811866861268088RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC383521.089434654089158RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG368491.0467401326796877RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT322880.9171794459540762RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT313760.8912729898493278RNA PCR Primer, Index 1 (97% over 35bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA308060.87508145478386RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG196510.5582102729324687RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC186070.5285541981809805RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA157040.4460909941545718No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG156650.4449831522816713RNA PCR Primer, Index 3 (97% over 37bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC136390.38743218729458767RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC129420.3676330645917262RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC128900.3661559420945256RNA PCR Primer, Index 38 (100% over 38bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA128840.36598550488331016RNA PCR Primer, Index 38 (100% over 40bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA119950.3407323914215543RNA PCR Primer, Index 38 (100% over 40bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG112150.31857555396354575RNA PCR Primer, Index 3 (97% over 37bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG110170.3129511259934359RNA PCR Primer, Index 1 (100% over 29bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTC107130.3043156406251864RNA PCR Primer, Index 38 (100% over 51bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA103250.2932940342999206No Hit
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT98730.2804544310550234RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT90570.25727497032972213RNA PCR Primer, Index 38 (100% over 39bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC89840.25520131759326753No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA87290.24795773611661084RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT87000.24713395626240284RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG86260.24503189732407896No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCG84870.24108343526425438RNA PCR Primer, Index 38 (100% over 51bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA84010.23864050190349956RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC83430.23699294219508357RNA PCR Primer, Index 1 (100% over 24bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG83200.236339599552091RNA PCR Primer, Index 3 (97% over 37bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG82460.23423754061376711RNA PCR Primer, Index 3 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT82160.23338535455768986RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC80080.2274768645688876RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC79680.2263406164941179RNA PCR Primer, Index 2 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG79170.22489190019878658RNA PCR Primer, Index 1 (100% over 29bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC76280.21668250785857576RNA PCR Primer, Index 1 (100% over 33bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC72830.20688236821368736RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG72810.20682555580994888RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC72160.20497915268844819No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC71720.20372927980620154RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71060.20185447048283156RNA PCR Primer, Index 1 (100% over 33bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC69770.1981900704416994RNA PCR Primer, Index 38 (100% over 38bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA69750.19813325803796092No Hit
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT68670.19506538823608283RNA PCR Primer, Index 38 (100% over 39bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA67950.1930201417014974RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC66880.18998067810148853RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA66520.18895805483419584No Hit
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC65630.1864299028678333RNA PCR Primer, Index 2 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT64040.1819133167706239RNA PCR Primer, Index 1 (97% over 35bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC63160.17941357100613062RNA PCR Primer, Index 1 (100% over 33bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA62360.1771410748565913RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT60740.17253927015377413RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC59460.1689032763145112RNA PCR Primer, Index 1 (100% over 24bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA57840.16430147161169403RNA PCR Primer, Index 2 (97% over 36bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA57390.16302319252757816RNA PCR Primer, Index 38 (100% over 40bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA56430.16029619714813095RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC56240.15975647931261536RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA55700.15822254441167632RNA PCR Primer, Index 2 (97% over 36bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC55180.15674542191447574RNA PCR Primer, Index 1 (100% over 31bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT55130.15660339090512954RNA PCR Primer, Index 1 (97% over 35bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC54750.15552395523409834RNA PCR Primer, Index 2 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC50940.14470119232191725RNA PCR Primer, Index 1 (100% over 33bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC50270.14279797679667808RNA PCR Primer, Index 1 (100% over 26bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG49230.13984373180227694RNA PCR Primer, Index 1 (100% over 29bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC48650.1381961720938609RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC46420.13186158907702RNA PCR Primer, Index 1 (100% over 33bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA46180.13117984023215823RNA PCR Primer, Index 38 (100% over 40bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC45660.12970271773495762RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG45380.12890734408261886Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG44080.12521453783961745No Hit
CTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCT44030.12507250683027124RNA PCR Primer, Index 38 (100% over 51bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC43530.12365219673680916RNA PCR Primer, Index 38 (100% over 38bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA43130.12251594866203948RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA41480.1178289253536146No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG41310.11734601992183749RNA PCR Primer, Index 1 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG41030.11655064626949874No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG40730.11569846021342146RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC40710.11564164780968299No Hit
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA40460.11493149276295195RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA40020.11368161988070533RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA39350.11177840435546611RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA38700.1099320012339654No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC38080.10817081671807241RNA PCR Primer, Index 1 (100% over 27bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC37730.10717659965264897RNA PCR Primer, Index 38 (100% over 38bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT36930.10490410350310962RNA PCR Primer, Index 1 (100% over 25bp)
TACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT36430.10348379340964753RNA PCR Primer, Index 38 (100% over 39bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC36210.10285885696852423RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA35630.1012112972601082RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA35600.10112607865450049No Hit
TTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA35560.10101245384702351RNA PCR Primer, Index 1 (100% over 32bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTAT35470.10075679803020034RNA PCR Primer, Index 38 (100% over 41bp)
TCGTACAACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC35460.1007283918283311RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph