FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924617
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC2222313.209289409074899RNA PCR Primer, Index 7 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1278501.8463114999717671RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1194591.7251351229966942RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG950731.3729712415863577RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA933871.3486233245824282RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC761691.099974193518573RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG742291.0719582036089506RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA721291.0416316165933799No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC721071.041313909491312RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG665730.9613961320893271RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC474220.6848320997392346RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA456830.6597187974439597RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG376470.5436690577977092RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA360810.5210540886232409RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG348720.5035946392414195RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG343240.4956808441535467RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA315380.45544757204622294No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC305600.4413240472361143RNA PCR Primer, Index 7 (100% over 44bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATC282900.40854245079547363RNA PCR Primer, Index 7 (100% over 42bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC277080.40013765382258687No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT271780.3924838009091334RNA PCR Primer, Index 7 (100% over 41bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA255200.3685402383987446RNA PCR Primer, Index 7 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA238860.34494326545424825RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG231770.3347044320285151RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA223380.322588238454199RNA PCR Primer, Index 7 (100% over 37bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT223100.32218388396065806RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA215860.3117284320562423No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC212700.3071650027719945RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC210970.30466666965118794RNA PCR Primer, Index 2 (100% over 34bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG207220.2992512076841218RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT204220.2949188381104688RNA PCR Primer, Index 1 (100% over 30bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT199010.28739495628422485RNA PCR Primer, Index 7 (100% over 41bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA198100.28608080418021675RNA PCR Primer, Index 7 (100% over 40bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC193320.27917789532619636RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG190150.2746000248100364RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA179510.2592345540554806RNA PCR Primer, Index 7 (100% over 35bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC165850.2395078312634475RNA PCR Primer, Index 7 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG164740.2379048545211959RNA PCR Primer, Index 1 (100% over 29bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG162400.2345256062537466No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT159160.2298466471142014RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC158590.22902349689520735RNA PCR Primer, Index 1 (100% over 27bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC158360.22869134856122728RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT155270.22422900790036474RNA PCR Primer, Index 7 (100% over 46bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC153450.22160070369234863RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG153310.22139852644557814RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC148600.214596706214943RNA PCR Primer, Index 1 (100% over 33bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC143990.20793929830342966RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA141310.20406904815096633RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT139140.20093530082602404RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC139000.20073312357925355No Hit
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT137770.19895685205405583RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG137430.1984658501690418RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA136040.19645851893324928RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT134020.19354139008698965RNA PCR Primer, Index 1 (100% over 30bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133440.19270379863608342RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC126700.18297040832727646RNA PCR Primer, Index 2 (100% over 34bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT124530.17983666100233414RNA PCR Primer, Index 7 (100% over 38bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA124490.17977889607468542RNA PCR Primer, Index 7 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG123890.17891242215995484No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA123290.17804594824522424No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA120100.17343919526523993RNA PCR Primer, Index 7 (100% over 35bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG117320.16942453279365485RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG116610.16839920532789035Illumina Small RNA Adapter 2 (100% over 21bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC115470.16675290488990221RNA PCR Primer, Index 7 (100% over 39bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC114450.16527989923486022No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC112260.16211726944609356No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG112230.16207394575035702RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG108420.15657183639181776RNA PCR Primer, Index 7 (100% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG107360.15504106580912705RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA106940.15443453406881563RNA PCR Primer, Index 1 (100% over 32bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG103320.14920680811660775RNA PCR Primer, Index 7 (100% over 36bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA103220.14906239579748598RNA PCR Primer, Index 7 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG102690.14829701050614064Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT100880.14568314753003667RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA100000.14441231912176514RNA PCR Primer, Index 7 (100% over 35bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG97250.14044098034591662Illumina Small RNA Adapter 2 (100% over 21bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC95420.1377982349059883RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT95220.13750941026774477RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA94910.1370617320784673No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT94190.1360219633807906RNA PCR Primer, Index 7 (100% over 38bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG94010.13576202120637143Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC93790.13544431410430352RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC91940.13277268620055088RNA PCR Primer, Index 2 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG91150.13163182887948893RNA PCR Primer, Index 7 (100% over 36bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA90070.13007217583297387RNA PCR Primer, Index 7 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC90030.13001441090532517RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC88320.12754496024834297No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG87950.12701063466759246RNA PCR Primer, Index 7 (100% over 45bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC86820.1253787754615165RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT86780.1253210105338678RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG86640.12511883328709733Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA85710.12377579871926492RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC85510.12348697408102137RNA PCR Primer, Index 1 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG82530.11918348697119278RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA82420.11902463342015883RNA PCR Primer, Index 7 (100% over 37bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC80800.11668515385038623RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA80630.11643965290787923RNA PCR Primer, Index 7 (100% over 37bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA80460.11619415196537222RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC79750.11516882449960772RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG76860.1109953084769887RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG76790.11089421985360345RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC76470.11043210043241382RNA PCR Primer, Index 1 (100% over 31bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC75740.10937789050282493RNA PCR Primer, Index 7 (100% over 39bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA74400.10744276542659327RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG73620.10631634933744351RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT73480.10611417209067305RNA PCR Primer, Index 1 (100% over 25bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC72610.10485778491431366RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT70220.10140633048730348RNA PCR Primer, Index 7 (100% over 43bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC70210.1013918892553913RNA PCR Primer, Index 1 (100% over 33bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG69560.10045320918109983RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC69550.10043876794918766RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATA750.045.2412071
GCCCGAC351.16317096E-745.2412031
GTCTACG178350.045.1777881
TCCTTCA245200.045.1489521
CAACGGA47950.045.0996781
TGAGGTA173250.045.071471
ATCTAAA24800.045.058781
ATTACGT600.044.99878721
TATCTCA750.044.99878723
TATTACG551.8189894E-1244.99521320
CCGTTTA1350.044.9948917
GATAGGG2200.044.99456415
TACGTAA351.2145392E-744.9932677
ATTCGAT207.040938E-444.9932638
TCGCTAA551.8189894E-1244.9932638
AATCGTG2250.044.9932639
TCTATAG207.040938E-444.99326313
TAATCGT2250.044.9932638
TTCACAG139300.044.916431
TGTAAAC66200.044.8995061
Produced by FastQC (version 0.11.5)