FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455482
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1259843.645916835914642RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC965302.7935321324203106RNA PCR Primer, Index 47 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA580101.6787817155464853RNA PCR Primer, Index 47 (100% over 37bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG542061.5686957709517804RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC401541.1620375970703942RNA PCR Primer, Index 5 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC334070.9667826369808902RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC289690.8383490349537344RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC288910.8360917521781333No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC258490.7480577239296862RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA230680.667576911122674RNA PCR Primer, Index 1 (100% over 28bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTC229980.6655511445291858RNA PCR Primer, Index 47 (100% over 51bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG215120.6225470137017065RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA196930.5699060217937758RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG196550.5688063199287393RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG191880.5552915627978963RNA PCR Primer, Index 10 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA190450.5511532110426274No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA185210.5359889011142295RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG178880.5176701832045428RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG175750.5086121125793739RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC175120.5067889226452345RNA PCR Primer, Index 5 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG174790.5058339183940186RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG171030.4949526578347102RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT152480.4412698431072713RNA PCR Primer, Index 3 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT143160.41429820789111327RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA138780.4016226969204296RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCG136200.3941563000472872RNA PCR Primer, Index 47 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA132490.38341973710179944No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA127420.3687473990603916RNA PCR Primer, Index 47 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG124160.3593131146392891RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA117660.3405024248426124RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG116340.33668240783774883RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG114130.3302867733068788RNA PCR Primer, Index 47 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA107900.31225745062483323No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG96090.27807987424040986RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC92970.26905074313800503RNA PCR Primer, Index 1 (100% over 33bp)
CTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCT92390.26737225081768623RNA PCR Primer, Index 47 (100% over 51bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT91160.2638126895176997RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG86070.24908247243076365RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG85830.24838792388442482RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG84910.24572548779012593RNA PCR Primer, Index 10 (97% over 36bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT83350.24121092223892354RNA PCR Primer, Index 3 (100% over 34bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG81160.23487316675358172RNA PCR Primer, Index 47 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGT76420.22115583296338975RNA PCR Primer, Index 47 (100% over 46bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG75530.21858021543738324RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA71150.2059047044666996No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT69310.20057983227810186RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA68870.1993064932764807RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA68230.19745436381957715RNA PCR Primer, Index 47 (100% over 40bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC66620.19279510065455413No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT65780.19036418074236822RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA65530.18964069267326528No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG65330.1890619022179829RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG63900.18492355046271403RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC62940.1821453562773587No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC62750.18159550534484045No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC60310.17453426179039566No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT60070.17383971324405684RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG59900.17334774135706685RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA59360.17178500712780445No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGAT58820.17022227289854208RNA PCR Primer, Index 47 (100% over 41bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA58490.1692672686473262RNA PCR Primer, Index 47 (100% over 40bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT57360.16599710257498088RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC55540.16073010943191138No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC54320.157199487654689RNA PCR Primer, Index 47 (100% over 44bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG54140.15667857624493486RNA PCR Primer, Index 1 (100% over 29bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCT52370.15155628071568597RNA PCR Primer, Index 47 (100% over 51bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC51280.14840187273439712No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA50380.14579731568562648RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA48370.13998047161003876No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCT48240.13960425781410524RNA PCR Primer, Index 47 (100% over 43bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC47520.13752061217508876RNA PCR Primer, Index 47 (100% over 42bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA45970.13303498614665044RNA PCR Primer, Index 47 (100% over 37bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT43630.12626313781984683RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC43420.12565540784180038No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTC43320.12536601261415917RNA PCR Primer, Index 47 (100% over 51bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG42890.12412161313530212No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC41850.12111190276783385RNA PCR Primer, Index 47 (100% over 42bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC41420.11986750328897677RNA PCR Primer, Index 1 (100% over 24bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA40970.11856522476459146RNA PCR Primer, Index 47 (100% over 38bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA40300.11662627673939555No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG40060.11593172819305672RNA PCR Primer, Index 10 (97% over 36bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT39760.11506354251013316RNA PCR Primer, Index 3 (100% over 34bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA38930.11266156212071138RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA38830.1123721668930702RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC37520.10858108941097075RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT37270.1078576013418678RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA36880.10672895995406718RNA PCR Primer, Index 1 (100% over 32bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG36860.10667108090853897RNA PCR Primer, Index 47 (100% over 45bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36710.10623698806707718RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC36510.10565819761179482RNA PCR Primer, Index 1 (100% over 27bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36500.10562925808903072RNA PCR Primer, Index 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)