FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968320
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA10613510.960736120290813No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA368153.801945637805684No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC308233.1831419365499007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC287992.974120125578321No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA249172.573219596827495No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA226112.3350751817580964No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC210722.1761401189689358No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA207262.1404081295439523No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA188781.9495621282220754No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC178671.8451544943820224No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC123361.2739590218109715RNA PCR Primer, Index 1 (100% over 51bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC107181.1068654990085922No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG93520.9657964309319234No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA85250.8803907799074686No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC85180.8796678783873101RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA79460.8205964970257766No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC73010.7539862855254461No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC72800.7518175809649702RNA PCR Primer, Index 1 (100% over 36bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG71470.7380824520819564RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA63040.6510244547257105No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA58600.6051718440185063No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG55580.5739838070059485RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG54400.5617977528089888RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA53950.5571505287508262No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA52330.5404205221414408No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA45240.46720092531394575No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC44110.4555312293456708RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG43470.44892184401850627No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA38730.3999710839391936No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA38560.39821546596166557RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG37380.3860294117647059RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT35570.36733724388631855RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA34190.35308575677461995No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG30360.31353271645736946RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA29590.3055807997356246RNA PCR Primer, Index 1 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG25630.2646852280237938RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT24910.25724966953073364RNA PCR Primer, Index 1 (100% over 35bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA24530.2533253469927297RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCCA24300.2509500991407799No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC24210.2500206543291474RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGGAATTCTCGGGTGCCA23370.2413458360872439No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG22380.2311219431592862No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG21550.22255039656311962Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGGATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG20960.2164573694646398RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA19610.20251569729015204No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA19160.19786847323198942No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG18480.19084600132187707RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG17890.18475297422339723RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC17700.18279081295439525No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC16820.17370290812954395RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGCC16810.173599636483807No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT16810.173599636483807RNA PCR Primer, Index 1 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC16790.17339309319233312No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCCA16070.16595753469927296No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTGGAATTCTCGGGTGCCA15710.1622397554527429No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTTGGAATTCTCGGGTGCC15600.1611037673496365No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA15370.15872851949768674RNA PCR Primer, Index 1 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTTGGAATTCTCGGGTGCCA15240.1573859881031064No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA14780.15263549239920687No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT14090.14550974884335757RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA13410.1384872769332452RNA PCR Primer, Index 1 (100% over 28bp)
CACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCACCATGGAATTCTCG13240.13673165895571712No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT13170.13600875743555849RNA PCR Primer, Index 1 (100% over 41bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA12930.13353023793787178No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC12210.12609467944481162RNA PCR Primer, Index 1 (100% over 38bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG12190.12588813615333774RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA12060.12454560475875744RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG11860.12248017184401852RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT11840.1222736285525446RNA PCR Primer, Index 1 (100% over 46bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCCA11450.1182460343688037No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG11140.11504461335095836Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA11050.11411516853932584RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG10890.1124628222075347RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG10840.11194646397884997No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA10110.10440763384005289RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT10000.10327164573694647RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC9730.10048331130204892RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT453.6743586E-1045.2119561
GTTTCCA850.045.2119561
CAACGGA650.045.2119561
AACCCGT253.7838676E-545.2119561
GTCTACG1550.045.2119561
GCATTAG1700.045.2119561
AGGCAAG850.045.2119561
TCGTACC502.0008883E-1145.2119561
TTGCAGT502.0008883E-1145.2119561
AGCTACA900.045.2119561
TCCCATA253.7838676E-545.2119561
TCCCACA551.8189894E-1245.2119561
TAATACT800.045.2119521
TATTGCA9150.045.2119521
GCATTGA206.8717904E-445.2119521
TAGGATG302.0962198E-645.2119521
TCCTTCA13200.045.2119521
GGGGGTA2550.045.2119521
GCCCGAC1150.045.2119521
GTAGTCG206.8717904E-445.2119521
Produced by FastQC (version 0.11.5)