FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3754346
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC1113332.9654432489706597RNA PCR Primer, Index 25 (100% over 51bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA349510.9309477602756913RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG345740.9209060645982016RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT323150.8607357979259237RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA317540.8457931154986781RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA308740.8223536136520182RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC303590.8086361779122117RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA301270.8024566728799103RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA296660.790177570207967RNA PCR Primer, Index 25 (100% over 38bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG275500.7338162225857713RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG267590.7127473067213304RNA PCR Primer, Index 5 (97% over 37bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC266530.70992391218071RNA PCR Primer, Index 25 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA257400.6856054290148005No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA216050.5754664061330522RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG214080.5702191540151068RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA212490.5659840622041762RNA PCR Primer, Index 25 (100% over 40bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT192230.5120199363617525RNA PCR Primer, Index 25 (100% over 41bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG191800.5108745970669725RNA PCR Primer, Index 5 (97% over 37bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG188600.5023511418500053RNA PCR Primer, Index 5 (97% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG187880.5004333644261877RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC176340.4696956540499997RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA158900.42324282311752837RNA PCR Primer, Index 1 (100% over 22bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT155740.4148259110907732RNA PCR Primer, Index 8 (100% over 36bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT152090.40510384498391994RNA PCR Primer, Index 8 (100% over 36bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT149520.3982584450127931RNA PCR Primer, Index 25 (100% over 39bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA147080.3917593104098557RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA142790.38033255325960896RNA PCR Primer, Index 1 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC140500.37423295561996683RNA PCR Primer, Index 1 (100% over 33bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA136280.3629926490525913RNA PCR Primer, Index 25 (100% over 40bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC134220.35750567475666867RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT132610.353217311350632RNA PCR Primer, Index 1 (100% over 30bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG131940.35143271291457956RNA PCR Primer, Index 1 (100% over 29bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA130930.34874249736172425RNA PCR Primer, Index 25 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA126510.33696947484328826No Hit
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC126430.3367563884628641RNA PCR Primer, Index 5 (100% over 35bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA124850.33254793244948655RNA PCR Primer, Index 25 (100% over 38bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG120770.3216805270478533RNA PCR Primer, Index 5 (97% over 37bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC120510.32098799631147473RNA PCR Primer, Index 5 (100% over 35bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC118650.31603373796661255RNA PCR Primer, Index 25 (100% over 44bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC116630.31065330686090203No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115980.3089219800199555RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC115770.3083626282713421RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA115160.30673784462060766No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC110030.2930736804759071RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT107450.2862016447072273RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG100290.26713041365926316RNA PCR Primer, Index 25 (100% over 45bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA100190.2668640556837329RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC99000.26369439577492326RNA PCR Primer, Index 1 (100% over 24bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC98260.2617233467559996RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG97660.26012519890281827RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC97460.2595924829517578RNA PCR Primer, Index 5 (100% over 35bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC95570.2545583172142365RNA PCR Primer, Index 1 (100% over 31bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT95080.25325316313413837RNA PCR Primer, Index 25 (100% over 41bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT92710.24694047911407208RNA PCR Primer, Index 25 (100% over 39bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC92020.24510260908291354RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG91290.24315819586154286No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC86680.23087909318959945RNA PCR Primer, Index 5 (100% over 35bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA86080.2292809453364181RNA PCR Primer, Index 25 (100% over 38bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC85940.2289080441706758RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA84820.22592483484473727RNA PCR Primer, Index 1 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG81650.21748128702042913RNA PCR Primer, Index 1 (100% over 29bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT81510.2171083858546868RNA PCR Primer, Index 25 (100% over 39bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA80860.21537705901374035RNA PCR Primer, Index 1 (100% over 32bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA79450.21162141155876418RNA PCR Primer, Index 1 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC78930.210236350086007RNA PCR Primer, Index 1 (100% over 33bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA77650.20682696799922012RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA76130.20277832677116067RNA PCR Primer, Index 25 (100% over 40bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA74110.19739789566545013RNA PCR Primer, Index 25 (100% over 40bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG72860.1940684209713223Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA70580.18799545912923316RNA PCR Primer, Index 1 (100% over 34bp)
AAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT70290.1872230210001955RNA PCR Primer, Index 25 (100% over 43bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT70240.1870898420124304RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT69440.18495897820818857RNA PCR Primer, Index 25 (100% over 46bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA69360.1847458918277644RNA PCR Primer, Index 1 (100% over 34bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA68640.18282811440394678No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT68140.18149632452629566RNA PCR Primer, Index 25 (100% over 39bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC67990.18109678756300032RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG67500.17979163348290222RNA PCR Primer, Index 1 (100% over 29bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT66890.17816684983216782RNA PCR Primer, Index 25 (100% over 39bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA66840.17803367084440272RNA PCR Primer, Index 25 (100% over 38bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG64150.17086864130263965No Hit
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA63610.16943030823477642RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG61010.1625050008709906RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT59360.15811009427474185RNA PCR Primer, Index 1 (100% over 30bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT58600.15608577366071213RNA PCR Primer, Index 8 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT57740.15379509507115222RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC55620.14814830598991144RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA55000.14649688654162402No Hit
GTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT54280.14457910911780641RNA PCR Primer, Index 25 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG52870.14082346166283022Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT52490.13981130135581535RNA PCR Primer, Index 25 (100% over 41bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC51690.13768043755157355RNA PCR Primer, Index 5 (100% over 35bp)
CTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT51390.13688136362498288RNA PCR Primer, Index 25 (100% over 39bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC50190.13368506791862017RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA50180.13365843212106715No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC50020.1332322593602188RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG49890.1328859939920295RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC49870.13283272239692348RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT49540.13195374107767371RNA PCR Primer, Index 1 (100% over 25bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA49310.13134111773395418RNA PCR Primer, Index 25 (100% over 38bp)
ATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGA47530.1265999457695162RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT47440.12636022359153898RNA PCR Primer, Index 25 (100% over 41bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC46280.12327047107538835No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA45020.11991436058370751No Hit
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA44940.11970127420328336RNA PCR Primer, Index 25 (100% over 38bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC44770.11924846564488195RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA44540.11863584230116245RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT44490.11850266331339733RNA PCR Primer, Index 1 (100% over 25bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT44440.11836948432563221RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC44200.11773022518435966No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG43990.1171708734357462No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC42120.112189979293331RNA PCR Primer, Index 5 (100% over 35bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC40880.10888714039675618RNA PCR Primer, Index 1 (100% over 24bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA40330.10742217153133994RNA PCR Primer, Index 1 (100% over 34bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG39180.10435905481274235RNA PCR Primer, Index 5 (97% over 37bp)
CTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC38130.10156229606967498RNA PCR Primer, Index 25 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACATC302.0886982E-645.240681
CGCGATC302.0886982E-645.240681
GAAAAAG206.8528624E-445.240681
TTACGTC302.0886982E-645.240681
TGAGGTA22300.045.240681
TCCCACA1050.045.240681
GGGGGTA406.508344E-945.240681
AGTAAGG2600.045.240681
GTCTACG69200.045.1753041
TCCTTCA56800.045.1610341
AACGAGC351.2133751E-744.99784522
CCCTAAA253.8942155E-544.99664317
AGCCCGA207.0379174E-444.99664326
CCTAAAC253.8942155E-544.99664318
CTAAACG253.894828E-544.99544519
CCAGGTA207.039303E-444.99484614
CGTGATA1050.044.99484612
CAAATAC207.039303E-444.99484612
GATAGGG1000.044.99484615
GACAGCG302.1689302E-644.9936511
Produced by FastQC (version 0.11.5)