FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186980
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC138509343.46098814551707RNA PCR Primer, Index 14 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC430721.3514989111949243RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG343531.0779170248950416RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCCG201260.6315069438779033RNA PCR Primer, Index 14 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC171980.5396331323070744RNA PCR Primer, Index 1 (100% over 27bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG150010.4706963959610666RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA146530.4597769675366648RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA142140.4460021713346177RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT133760.419707685645972RNA PCR Primer, Index 8 (97% over 37bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG133470.4187977332772719RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA131580.4128673540467778RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG116960.36699320359713583RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA116820.3665539162467289RNA PCR Primer, Index 1 (100% over 28bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT95970.30113147870397683RNA PCR Primer, Index 8 (97% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC87080.2732367319531343RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG86160.2703499865076028RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA85940.26965967781410616RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC85850.2693772788031302RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC83260.2612504628206013RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC81400.2554142165937659RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA80480.2525274711482344No Hit
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC77770.24402412315107092RNA PCR Primer, Index 14 (98% over 51bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA75620.2372779245555353RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA73940.23200647635065172No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT73660.23112790164983776RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC71050.22293833033153643RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC69990.21961229753559797No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC68910.21622350940388707RNA PCR Primer, Index 1 (100% over 26bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA65870.20668469836647863RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA65790.2064336770233889RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC65640.20596301200509573RNA PCR Primer, Index 14 (98% over 51bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTC65450.20536683631525768RNA PCR Primer, Index 14 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT64950.2037979529209471RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTACAGTCACAGTTCCATCTCGTATGCC59220.1858185492221476RNA PCR Primer, Index 14 (98% over 51bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC57770.18126878737864685RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA51030.16012023922333996RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG50560.158645488832688RNA PCR Primer, Index 2 (97% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA49360.15488016868634255RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC49300.15469190267902527RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTTCAGTCACAGTTCCATCTCGTATGCC48420.15193066790503862RNA PCR Primer, Index 14 (98% over 51bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG48270.15146000288674544RNA PCR Primer, Index 2 (97% over 35bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC47980.1505500505180453RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA47640.14948320980991409RNA PCR Primer, Index 14 (100% over 40bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT47630.1494518321420279RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT47220.1481653477586932RNA PCR Primer, Index 8 (97% over 37bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG46650.14637682068917912Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA46350.14543549065259273No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG45360.14232910153185774No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG44610.13997577644039183RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC43360.13605356795461535RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG43050.13508086025014276RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT41470.13012318872412126RNA PCR Primer, Index 8 (97% over 36bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA41190.12924461402330734RNA PCR Primer, Index 1 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT39910.1252282725338722RNA PCR Primer, Index 1 (100% over 25bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG39450.12378489981110645RNA PCR Primer, Index 2 (97% over 35bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC38900.12205912807736478RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG37510.11769763224118131RNA PCR Primer, Index 2 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATTTCGTATGCC35800.11233205103263905RNA PCR Primer, Index 14 (98% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG35420.111139699652963RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC35330.11085730064198708No Hit
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA34840.10931979491556269RNA PCR Primer, Index 1 (100% over 34bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC34690.10884912989726951RNA PCR Primer, Index 14 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT34380.10787642219279693RNA PCR Primer, Index 8 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA34340.10775091152125209No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG33870.10627616113060012RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA33600.10542896409767241RNA PCR Primer, Index 1 (100% over 34bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC33050.10370319236393075RNA PCR Primer, Index 14 (100% over 44bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC32200.10103609059360272RNA PCR Primer, Index 1 (100% over 31bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT32070.10062818091108197RNA PCR Primer, Index 8 (97% over 37bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT32010.10043991490376471RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGG1100.045.2501071
TTCTACG253.7670303E-545.2501031
TTAATGC253.7670303E-545.2501031
ATCTAAA900.045.2501031
CATTATT750.045.2501031
ACGTGGG206.8456784E-445.2501031
GCATCGT253.7670303E-545.2501031
TCCTTCA52950.045.2073751
GTCTACG41500.045.1410641
GTTGACC207.038303E-444.99593718
CTATGTT207.038303E-444.99593718
TAGTTTT253.894444E-544.99593718
AATGCGC351.2137025E-744.99593722
ATGCGCC351.2137025E-744.99593723
ACTCGTG207.0388464E-444.99522820
GCGGTTA502.1827873E-1144.99452615
TTCACTC302.1685337E-644.99452615
ATTACCA351.2139935E-744.99452616
GCTGCCC650.044.99452612
AGCGGTA453.8562575E-1044.99452614
Produced by FastQC (version 0.11.5)