FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435157
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1508416.194302872463664RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA915023.7575400682584323No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA893673.669866049704393No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA724552.9753728404369824No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC493562.0268097703762016RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA291231.1959393172596264RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATCTCGTATGCC242120.9942685420282963RNA PCR Primer, Index 45 (100% over 51bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG218710.8981351099744288No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG185480.7616757358971106RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC184080.7559266199263538RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC181050.7434838903610732No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG173260.7114941664952198RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA172420.7080446969127658No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC172150.7069359388326913RNA PCR Primer, Index 5 (97% over 35bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG166940.6855410143986609RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG137640.5652202301535384RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC120660.4954916664510748RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC120240.4937669316598478RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG118510.48666266692455556RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG112790.4631734216726067No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA100630.413238242955177RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG95610.3926235556886065RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT92390.3794005889558661RNA PCR Primer, Index 3 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA92160.37845609133209895No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT88800.36465821300228285RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC88700.36424756186151447RNA PCR Primer, Index 1 (100% over 27bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC82270.3378426935101104RNA PCR Primer, Index 1 (100% over 33bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC76570.31443557848631526No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCAT75890.31164315072909055RNA PCR Primer, Index 42 (97% over 37bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTC75720.3109450437897844RNA PCR Primer, Index 45 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG70140.2880307101349112RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG69560.2856489335184549No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA64300.26404868351404037No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC63820.26207755803835237No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG60600.2488545913056119RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA57920.23784914073302052No Hit
GTTTTCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA56710.23288026192972364No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC56570.232305350332648RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG55970.22984144348803795RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCAT55920.22963611791765376RNA PCR Primer, Index 45 (100% over 41bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA55810.2291844016628086No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG54310.22302463455128352RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCAT54030.22187481135713222RNA PCR Primer, Index 42 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG53670.22039646725036618No Hit
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT53510.2197394254251369RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT52160.21419563502476432RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC51210.2102944491874651RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA50360.20680391449093427RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCAT50340.20672178426278057RNA PCR Primer, Index 45 (100% over 41bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT49950.20512024481378408RNA PCR Primer, Index 1 (100% over 30bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC49800.20450426810263156RNA PCR Primer, Index 5 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCATGTTCGCCTTGGAATTCTCGGGTGCCA47650.1956752685761123No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA44780.18388958083606108No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC43980.1806043717099144No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA43480.1785511160060727RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC43010.17662105564446154No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC42230.1734179767464685RNA PCR Primer, Index 5 (97% over 35bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCA41750.1714468512707805RNA PCR Primer, Index 3 (97% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA40430.16602625621263845No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT39820.16352128425395157RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC39650.1628231773146454No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC39350.1615912238923404No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA38790.15929157750403772RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT38680.15883986124919255RNA PCR Primer, Index 1 (100% over 25bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT38540.15826494965211688RNA PCR Primer, Index 3 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC37840.15539039166673854RNA PCR Primer, Index 1 (100% over 24bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT37480.1539120475599725RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC37300.15317287550658953No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG37160.15259796390951383RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG36790.15107855468867099RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA35810.1470541735091413No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG35140.14430281086599345Illumina Small RNA Adapter 2 (100% over 21bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC34980.14364576904076412RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCA34820.14298872721553477RNA PCR Primer, Index 1 (100% over 28bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATC34780.14282446675922744RNA PCR Primer, Index 45 (100% over 42bp)
GTATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA34690.14245488073253593No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCA34280.14077121105538576RNA PCR Primer, Index 45 (100% over 40bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC32700.13428292303124603RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA32350.13284564403855684RNA PCR Primer, Index 1 (100% over 32bp)
GTATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA32070.1316958208444055No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC31830.1307102581065615RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG31830.1307102581065615RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA31430.12906765354348818No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG31310.12857487217456615Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA31240.1282874163760283No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA30880.1268090722692623RNA PCR Primer, Index 1 (100% over 22bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC30780.12639842112849398RNA PCR Primer, Index 1 (100% over 33bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC30680.12598776998772565RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG30670.12594670487364878Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA30360.12467368633726696RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG30180.12393451428388395RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG30020.12327747245865463Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA29990.12315427711642411No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC29560.12138847721112025RNA PCR Primer, Index 1 (100% over 24bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT29420.12081356561404459RNA PCR Primer, Index 3 (100% over 34bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT29300.12032078424512259RNA PCR Primer, Index 3 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCA29120.11958161219173959RNA PCR Primer, Index 3 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG28870.11855498433981873No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG28690.11781581228643574RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATCTCGT28590.1174051611456674RNA PCR Primer, Index 45 (100% over 46bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA28320.1162964030655929No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG28130.11551616589813304Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT28100.11539297055590256RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA27610.11338077996613771No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC27600.11333971485206087RNA PCR Primer, Index 5 (97% over 35bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATT27190.1116560451749107RNA PCR Primer, Index 45 (100% over 38bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCAT26990.11083474289337403RNA PCR Primer, Index 42 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA26970.11075261266522034RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC26300.1080012500220725RNA PCR Primer, Index 1 (100% over 26bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG26270.107878054679842RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC26010.10681036171384431RNA PCR Primer, Index 5 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA24970.10253958984985363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG551.8189894E-1245.0154081
TATGGCA2950.045.0154081
ATGTACG207.0229894E-445.0154081
AATGTGT551.8189894E-1245.0154081
AAAGATA253.8842365E-545.0154081
AGTAAGG3300.045.0154081
GCATAGG406.7993824E-945.0154081
GTCTACG54700.045.0154041
CATTAGT1050.045.008012
TATAAAG253.8880076E-545.008012
CCCATAT351.210974E-745.008012
ATCAGCC207.0300995E-445.00616526
TCGCCTA253.8889506E-545.0061627
CATATGC750.045.0061624
ATCACGC253.8889506E-545.0061621
AATGCGC502.1827873E-1145.0061622
ATGCGCC502.1827873E-1145.0061623
ATAGAGT207.0329447E-445.00246420
GATAGAG207.034368E-445.00061819
CATACTG253.8917817E-545.00061419
Produced by FastQC (version 0.11.5)